Antioxidative and Cardioprotective Effects of Schisandra chinensis Bee Pollen Extract on Isoprenaline-Induced Myocardial Infarction in Rats

Abstract

Oxidative stress plays an important role in the pathogenesis of myocardial infarction (MI). Schisandra chinensis bee pollen extract (SCBPE) possesses powerful antioxidant capacity. This study aimed to further explore the antioxidative and cardioprotective effects of SCBPE on acute MI induced by isoprenaline (ISO) in rats. The rats were intragastrically administrated with SCBPE (600, 1200, or 1800 mg/kg/day) and Compound Danshen dropping pills (270 mg/kg/day) for 30 days, then subcutaneously injected with ISO (65 mg/kg/day) on the 29th and 30th day. Compared with the model group, pretreatment with middle and high doses of SCBPE significantly reduced serum aspartate transaminase, lactate dehydrogenase, and creatine kinase activities and increased myocardial superoxide dismutase, glutathione peroxidase, and catalase activities. The histopathologic aspects showed that pathological heart change was found in the model group and reduced to varying degrees in the SCBPE groups. Moreover, the protein expression of nuclear factor-erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1), and Bcl2 in the heart increased in the SCBPE groups, while that of Bax decreased compared to the model group. Besides this, uridine was isolated from S. chinensis bee pollen for the first time. This study could provide a scientific basis for using Schisandra chinensis bee pollen as a functional food for the prevention of MI.

Schisandra chinensis bee pollen’s chemical profiles and protective effect against H2O2-induced apoptosis in H9c2 cardiomyocytes

“…In this study, GSH content and SOD activity significantly increased and MDA content decreased in H9c2 cardiomyocytes in the SCBPE groups compared with those in the H 2 O 2 group, suggesting that the protective effects of SCBPE against H 2 O 2 -induced myocardial cell injury could be related to antioxidant activity. These results were consistent with those of our previous research in which we reported that SCBPE could exert antioxidative and cardioprotective effects on isoprenaline-induced myocardial infarction in rats [18].…”

“…This property can also provide scientific evidence for using SCBPE as a functional food for the prevention of myocardial ischemia. With the exception of uridine, which has been isolated from S. chinensis bee pollen [18], the other chemical components of S. chinensis bee pollen remain unknown. Identification of these components is beneficial to the further development and utilization of functional foods from S. chinensis bee pollen.…”

“…Baill, which is often used as a functional food in China, has been found to possess a strong antioxidant activity [ 15 – 17 ]. Our recent study revealed the antioxidative and cardioprotective effects of S. chinensis bee pollen extract (SCBPE) on isoprenaline-induced myocardial infarction in rats [ 18 ]. However, its protective effects against H 2 O 2 -induced H9c2 cardiomyocyte injury have not been reported yet.…”

Phikud Navakot Modulates the Level of Pro-Inflammatory Mediators and the Protein Expression of SOD1 and 2 and the Nrf2/HO-1 Signaling Pathway in Rats with Acute Myocardial Infarction

“…There was also an increase in cardiac tissues and serum levels of the pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor- α (TNF- α ) [1, 4]. Moreover, the ISO-induced myocardial changes were the result of an increase in oxidative stress through reductions of the components of the myocardial antioxidant system, including glutathione, glutathione reductase, glutathione-S-transferase, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase, in rat hearts [3, 5]. Furthermore, it was shown that a reduction in heme oxygenase (HO)-1 levels and an induction of endogenous antioxidants occurred via the activity of nuclear factor erythroid 2-related factor 2 (Nrf2) [5].…”

Evaluation of the Effects of Schisandra chinensis on the Myocardium of Rats with Hyperthyroid Heart Disease by Using Velocity Vector Imaging Combined with the Estimation of p53 Expression and Calmodulin Activity

“…Its chemical components are mainly volatile oil, lignan, organic acids, polysaccharides, fatty oils, amino acids, pigments, and tannins. Previous studies found that Schisandra chinensis (SC) could improve myocardial ischemia and exert cardioprotective effect [5,6]. Here, we aimed to determine the changes in myocardial function in HHD rats treated with SC using VVI technique, combined with the estimation of the expression of N-terminal pro-brain natriuretic peptide (NT-proBNP) and p53 protein and calmodulin (CaM) activity, so that VVI could be developed as a valuable tool for evaluating myocardial function in HHD animals.…”