Antioxidative and Antiproliferative Activities of Different Horsetail (Equisetum arvense L.) Extracts

Abstract

In this study we investigated antioxidative and antiproliferative activity of different horsetail (Equisetum arvense L.) extracts. The antioxidative activity was measured by the electron spin resonance (ESR) spectroscopy-spin trapping method. The influence of different horsetail extracts during lipid peroxidation of (1) sunflower oil induced by the lipophilic azo-initiator 4,4'-azobis(4-cyanovaleric acid) and (2) soybean phosphatidylcholine liposomes induced by the hydrophilic azo-initiator 2,2'-azobis(2-amidinopropane) dihydrochloride was studied. Antiproliferative activity was measured using the sulforhodamine B colorimetric assay on the human cancer cell lines HeLa, HT-29, and MCF7. The results of ESR analysis confirmed that the extracts investigated suppressed the formation of lipid peroxyl radicals in both systems investigated in a dose-dependent manner. The results indicate that n-butanol, methanol, ethyl acetate, and water extracts had significant peroxyl radical scavenging activity. Extracts inhibited cell growth that was dependent on cell line, type of extract, and extract concentration. Ethyl acetate extract exhibited the most prominent antiproliferative effect, without inducing any cell growth stimulation on human tumor cell lines. The results obtained suggest that the horsetail extracts could be used as an easily accessible source of natural antioxidants and as potential phytochemicals.

The effect of stinging nettle and field horsetail extracts on the synthesis of biologically active compounds in germinated leguminous and quinoa seed

“…It is likely that a significant increase in antioxidant activity was associated with the composition of field horsetail extract. According to Cetojević-Simin et al (2010), field horsetail contains more than 10% inorganic substances (two-thirds of which are silicic acid and potassium salts). The results obtained suggest that the field horsetail extracts could be used as an easily accessible source of natural antioxidants and as potential phytochemicals (Cetojević-Simin et al, 2010, Romano et al, 2013.…”

“…According to Cetojević-Simin et al (2010), field horsetail contains more than 10% inorganic substances (two-thirds of which are silicic acid and potassium salts). The results obtained suggest that the field horsetail extracts could be used as an easily accessible source of natural antioxidants and as potential phytochemicals (Cetojević-Simin et al, 2010, Romano et al, 2013. Frias et al (2005) also have indicated that vitamin C increased sharply but gradually after germination and this processes caused a significant increase in Trolox equivalent antioxidant capacity (TEAC).…”

Estonian folk traditional experiences on natural anticancer remedies: From past to the future

“…Its extracts have shown significant anticancer activity against human prostate, breast, and colon cancer cells (Aydos et al, 2011;Durak et al, 2004;Guler, 2013;Konrad et al, 2000). Cytotoxicity toward several human cancer cell lines has demonstrated also for extracts prepared from Equisetum arvense L. (Equisetaceae) (field horsetail) and Secale cereale L. (Poaceae) (rye grass) (Cetojevic-Simin et al, 2010;Moodley & Shengwenyana;Sandhu et al, 2010); however, anticancer effects and potential oncological applications of these plants certainly need further investigation.…”

A Review of the Benefits of Satureja Species on Metabolic Syndrome and Their Possible Mechanisms of Action

“…The mechanism of scavenging free radical reactive species is suggested as being caused by the action of electron-hydrogen donation. 41 Cetojević-Simin et al 56 conducted an in vitro study in which they investigated the antioxidative effect of 6 S. montana extracts on free radicals. Antioxidative activity increased dose-dependently.…”