Antioxidative and Anti‐Inflammatory Effects of Lactobacillus plantarum ZS62 on Alcohol‐Induced Subacute Hepatic Damage

Abstract

Lactobacillus plantarum ZS62 is a newly isolated strain from naturally fermented yogurt that might offer some beneficial effects in the setting of alcohol-induced subacute liver injury. The liver-protective effect of L. plantarum ZS62 was investigated by gavage feeding of mice with this Lactobacillus strain ( 1 × 10 9 CFU/kg BW) before alcohol administration daily for 7 days. We then compared hepatic morphology, liver function indexes, liver lipid levels, inflammation, oxidative stress levels, and mRNA expression of oxidative metabolism- and inflammation-related genes in mice that had been pretreated with Lactobacillus plantarum versus control mice that had not been pretreated. Our results showed that L. plantarum ZS62 attenuated alcohol-induced weight loss; prevented morphological changes in hepatocytes; reduced markers of liver damage including aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), hyaluronidase (HAase), precollagen III (PC III), and inflammatory cytokines; and enhanced the antioxidative status. L. plantarum ZS62 also significantly downregulated inflammation-related genes and upregulated lipid- and oxidative-metabolism genes. Thus, Lactobacillus plantarum pretreatment appears to confer hepatic protection by reducing inflammation and enhancing antioxidative capacity. The protective effect of L. plantarum ZS62 was even better than that of a commonly used commercial lactic acid bacteria (Lactobacillus delbrueckii subsp. Bulgaricus). The L. plantarum ZS62 might be a potentially beneficial prophylactic treatment for people who frequently drink alcoholic beverages.

Lactobacillus plantarum ST-III culture supernatant ameliorates alcohol-induced cognitive dysfunction by reducing endoplasmic reticulum stress and oxidative stress

“…In the present study, we found that alcohol reduced the expression level of Nrf2 and SOD2 in mice, causing an imbalance in the redox system, while LP-cs reversed these effects ( Figures 5F–H ). Similar to our findings, a study found that L. plantarum ZS62 pretreatment appeared to confer hepatic protection on alcohol-induced subacute hepatic damage by enhancing antioxidative capacity ( Gan et al, 2021 ). In addition, oxidative stress is related to macromolecule damage and activation of several cell survival and death pathways.…”

“…Alcohol causes oxidative stress in the hippocampus (Zhao et al, 2021). When the antioxidant defense system is turned on, Nrf2 activates the transcription of antioxidant genes such as SOD2 (Hoang et al, 2019;Durymanov et al, 2020).…”

Modulating phenylalanine metabolism by L. acidophilus alleviates alcohol-related liver disease through enhancing intestinal barrier function

“…Long time Lactobacillus rhamnosus GG supplementation prevented alcohol-induced colonic dysbiosis [ 47 ]. Lactobacillus plantarum pretreatment relieved alcohol-induced subacute liver injury by reducing inflammation and enhancing antioxidative response of liver [ 48 , 49 ]. We found that Lactobacillus abundance was negatively correlated with the level of phenylalanine.…”

Alleviating Effect of Lacticaseibacillus rhamnosus 1.0320 Combined with Dihydromyricetin on Acute Alcohol Exposure-Induced Hepatic Impairment: Based on Short-Chain Fatty Acids and Adenosine 5′-Monophosphate-Activated Protein Kinase-Mediated Lipid Metabolism Signaling Pathway

“…Alcoholic beverages, which include distilled spirits, fermented spirits, and premixed spirits with an alcohol content of more than 0.5%, have long been consumed for social entertainment and leisure. 20 However, excessive alcohol consumption can cause a variety of health risks, including ALD, which is one of the most common liver diseases in the world. Therefore, the discoveries of bioactive components with potential to protect against acute alcoholic hepatic impairment and the development of rational prevention or treatment strategies have received increasing attention.…”