Antioxidative activity and protective effect of probiotics against high-fat diet-induced sperm damage in rats

Abstract

In this study, antioxidant capability and protective effect of probiotics on reproductive damage induced by diet oxidative stress were investigated. Thirty male Sprague-Dawley rats were randomly divided into three groups with 10 rats in each group. The control group consumed a normal standard diet (5% fat, w/w). The other two treatment groups were fed with a high-fat diet (20% fat, w/w), and a high-fat diet supplemented with 2% probiotics (w/w), respectively. At the end of the experimental period, that is, after 6 weeks, rats were killed. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), contents of nitric oxide (NO) free radical and malondialdehyde (MDA) in serum and sperm suspension were examined. Sperm parameters including sperm concentration, viability, motility and DNA integrity were analyzed. The results showed that high-fat diet could induce oxidative stress, shown as significant increases in lipid peroxidation, NO free radical, significant decrease in activities of SOD, GSH-Px, significant reduction in sperm concentration, viability and motility, and damage in sperm DNA ( P , 0.05), compared with the control group. These alterations were significantly reversed in the probiotics-supplemented group and had no significant difference in antioxidant capability, lipid peroxidation and sperm parameters compared with the control group. The percentage of sperm with DNA damage was significantly lower than the high-fat diet group and still higher than the control group, which means that probiotics could attenuate sperm damage to some extent. The present results indicated that dietary probiotics had antioxidant activity and the protective effect against sperm damage induced by high-fat diet to some extent.

Effects of Bacillus subtilis C‐3102 on production, hatching performance, egg quality, serum antioxidant capacity and immune response of laying breeders

“…With the development of modern intensive farming, chickens are prone to oxidative stress (Salami, Majoka, Saha, Garber, & Gabarrou, ). Numerous studies found that dietary probiotics in diets were beneficial in oxidation resistance, scavenging ROS and promoting antioxidant capability (Chen, Gong, & Xu, ; Singh, Pandey, & Naresh, ). Antioxidant enzymes (SOD, CAT, GSH and GSH‐Px) serve as the first line to suppress the oxidative damage.…”

“…Numerous studies found that dietary probiotics in diets were beneficial in oxidation resistance, scavenging ROS and promoting antioxidant capability (Chen, Gong, & Xu, 2013;Singh, Pandey, & Naresh, 2014). Antioxidant enzymes (SOD, CAT, GSH and GSH-Px) serve as the first line to suppress the oxidative damage.…”

Paternal Obesity, Interventions, and Mechanistic Pathways to Impaired Health in Offspring

“…anastrozole) in obese men can restore testosterone, LH, FSH, and estrogen levels as well as sperm function (count, motility, and morphology) [14]. Animal models of male obesity have shown improvements of both metabolic health (glucose, insulin, and cholesterol levels) and fertility measures (increased sperm count, increased sperm motility, and decreased sperm ROS and DNA damage) with the intake of selenium-enriched probiotics, olive oil, and metformin (diabetes medication) [15,16,17]. …”

The Consumption of Synbiotic Bread ContainingLactobacillus sporogenesand Inulin Affects Nitric Oxide and Malondialdehyde in Patients with Type 2 Diabetes Mellitus: Randomized, Double-Blind, Placebo-Controlled Trial

“…Furthermore, the administration of Lactobacillus fermentum (5 £ 10 8 CFU suspended in 0.5 ml of skim milk) in the trinitrobenzenesulfonic acid model of rat colitis for 3 weeks resulted in induced nitric oxide synthase (iNOS) expression [13]. However, any significant effects in NO, antioxidant capability, and lipid peroxidation was not seen following the consumption of probiotic supplements in rats after 6 weeks [14].…”