Antioxidative ability and membrane integrity in salt-induced responses of Casuarina glauca Sieber ex Spreng. in symbiosis with N2-fixing Frankia Thr or supplemented with mineral nitrogen

Campos, Paula Scotti; Duro, Nuno; Costa, Mário da; Pais, Isabel P.; Rodrigues, A. L.; Batista-Santos, Paula; Semedo, José N.; Leitão, António E.; Pawlowski, Katharina; Ramalho, José C.; Ribeiro‐Barros, Ana I.

doi:10.1016/j.jplph.2016.03.012

Cited by 22 publications

(32 citation statements)

References 46 publications

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“…In short, the level of salt tolerance in vitro did not predict the level of salt tolerance in planta. Nevertheless, it is also noteworthy that the tolerance limits of the C. glauca ecotype used by Ngom et al [9] were lower than the limits of the C. glauca ecotype used in our studies [4,12,21], i.e., 200 mM vs. 600 mM NaCl. Systems biology approaches are the best way to thoroughly dissect the complex interactions within biological systems, and the pathways involved in stress adaptation, as well as to deepen our understanding of the expression patterns and functions of the response-associated genes and proteins [20,27].…”

Section: Introductioncontrasting

confidence: 73%

“…Recently, the mechanisms underlying salt stress tolerance in C. glauca and the contribution of symbiotic nitrogen fixation have been thoroughly analysed by our team. For that, we have examined the impact of increasing NaCl concentrations (200, 400 and 600 mM) on plant growth, mineral contents, water relations, photosynthesis-related parameters and non-structural sugars [4,12,13], membrane integrity and the control of oxidative stress [21], as well as the metabolome of nodules, roots and branchlets from nodulated and non-nodulated C. glauca plants [7,8,22]. According to Batista-Santos et al [4], the C. glauca ecotype used in these studies exhibits outstanding salt stress tolerance showing the first stress symptoms, i.e., phenotypic changes (chlorosis and necrosis of branchlets, reduction of stem diameter and biomass decrease in both KNO 3 + and NOD + groups; and swollen nodules with salt crystals at the surface in NOD + plants), and the impairment of photochemical (e.g., photosynthetic electron flow) and biochemical (e.g., activity of photosynthetic enzymes) parameters only at 600 mM NaCl.…”

Section: Introductionmentioning

confidence: 99%

“…This tolerance is likely related to the control of the production of reactive oxygen species (ROS) in plant cells [22][23][24][25]. Indeed, photosynthetic branchlets of C. glauca showed a marked increase of the activity of several anti-oxidative enzymes (e.g., superoxide dismutase, ascorbate peroxidase, glutathione reductase and catalase), helping to preserve membrane stability up to 400 mM NaCl [21]. Notably, this tolerance seems to be largely independent of the presence of an active N 2 -fixing symbiosis, as revealed by the fact that at 200 mM NaCl the Frankia strain used (Thr) was no longer able to fix nitrogen in C. glauca nodules [12].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

Graça

Mendes

Marques

et al. 2019

IJMS

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Casuarina glauca displays high levels of salt tolerance, but very little is known about how this tree adapts to saline conditions. To understand the molecular basis of C. glauca response to salt stress, we have analyzed the proteome from branchlets of plants nodulated by nitrogen-fixing Frankia Thr bacteria (NOD+) and non-nodulated plants supplied with KNO3 (KNO3+), exposed to 0, 200, 400, and 600 mM NaCl. Proteins were identified by Short Gel, Long Gradient Liquid Chromatography coupled to Tandem Mass Spectrometry and quantified by Sequential Window Acquisition of All Theoretical Mass Spectra -Mass Spectrometry. 600 proteins were identified and 357 quantified. Differentially Expressed Proteins (DEPs) were multifunctional and mainly involved in Carbohydrate Metabolism, Cellular Processes, and Environmental Information Processing. The number of DEPs increased gradually with stress severity: (i) from 7 (200 mM NaCl) to 40 (600 mM NaCl) in KNO3+; and (ii) from 6 (200 mM NaCl) to 23 (600 mM NaCl) in NOD+. Protein–protein interaction analysis identified different interacting proteins involved in general metabolic pathways as well as in the biosynthesis of secondary metabolites with different response networks related to salt stress. Salt tolerance in C. glauca is related to a moderate impact on the photosynthetic machinery (one of the first and most important stress targets) as well as to an enhancement of the antioxidant status that maintains cellular homeostasis.

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Section: Introductioncontrasting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

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Mendes

Marques

et al. 2019

IJMS

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“…1 g fresh wild mustard and white mustard leaf samples of the control and treatment groups were weighed 3 times and extracted in 5 mL pH 7.8, 0.05 M sodium phosphate buffer in ice bath. This extract was centrifuged at 13000 rpm for 20 minutes in a cooled centrifuge as globally descibed in [21] with minor modifications.…”

Section: Determination Of the Total Protein Amountmentioning

confidence: 99%

“…The method of extraction which was used to determine total protein content was also performed to use the extract in the measurement of superoxide dismutase (SOD) activity [21].…”

Section: Determination Of Superoxide Dismutase Enzyme Activity 271 mentioning

confidence: 99%

The Allelopathic Effects of Sunflower and Wheat Root Exudates on Sinapis arvensis and Sinapis alba

Ünal¹,

Bayram²

2019

Phyton

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In this study, we aimed to investigate the allelopathic effects of sunflower and wheat root exudates on the common weeds such as wild mustard and white mustard in our region. The root exudates which were obtained by soaking 8 weeks old sunflower and wheat seedlings (20 or 40 seedlings) in 100 mL of distilled water for 3 days were applied to the leaves of wild mustard and white mustard. In order to compare the allelopathic effect, the recommended dose (1 g.da -1 ) and twice the recommended dose (2 g.da -1 ) of Gromstor (Tribenuron-methyl), a herbicide preferred by farmers for the chemical control of these weeds was also applied. The allelopathy was performed for wild mustard and white mustard seedlings by the measurement of different physiological and biochemical parameters, such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, proline, total protein amounts and superoxide dismutase enzyme activity. The amounts of total chl and carotenoid in wild mustard leaves decreased in all treatment groups compared to control. The highest decrease in total chl (50.93%) and carotenoid (46.69%) was oberved in the treatment of 40 wheat seedlings. 100 mL -1 distilled water. In the white mustard leaves, the amount of total chl in all treatment groups except the treatment group of Gromstor 2 g.da -1 and carotenoid in all treatment groups increased compared to the control. The highest increases again were observed in 40 wheat seedlings. 100 mL -1 distilled water treatment. The proline amounts in wild mustard and white mustard increased in all treatment groups. The highest increase was observed for the treatment of 20 wheat seedlings. 100 mL -1 distilled water in wild mustard (459.69%) and 40 sunflower seedlings. 100 mL -1 distilled water in white mustard plant (104.70%). In superoxide dismutase enzyme activities, treatments decreased activity except treatment of 40 sunflower seedling root exudate in wild mustard, while increased activity outside commercial herbicide treatment in white mustard. The results showed that sunflower and wheat root exudates have allelopathic effects on wild mustard and white mustard weeds. It is thought that the study will be a reference for new studies that will enable the use of plant root exudates as bioherbicides or foliar fertilizers and will contribute to the fight against weeds in organic agriculture. Materials and Methods MaterialsThe wild mustard (Sinapis arvensis L.) seeds collected from the fields in Imamoglu district of Adana province were assessed according to the Flora of Turkey and the East Aegean Islands [16]. The white mustard (Sinapis alba L.) seeds were purchased from Mersin Kemal Cuce Tarim Transport Food Construction Trade Industry Limited Company. The seeds of Sems sunflower (Helianthus annuus L.) and Sarıbasak wheat (Triticum aestivum L.) were obtained from the Eastern Mediterranean Agricultural Research Institute. All chemicals used in the study were purchased from Sigma-Aldrich and Merck companies. Obtaining the Root ExudatesThe sunflower and wheat root exudates were...

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Salt Stress Tolerance in Casuarina glauca and Its Relation with Nitrogen-Fixing Frankia Bacteria

Duro¹,

Costa²,

Batista-Santos³

et al. 2016

Biological Nitrogen Fixation and Beneficial Plant-Microbe Interaction

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Antioxidative ability and membrane integrity in salt-induced responses of Casuarina glauca Sieber ex Spreng. in symbiosis with N2-fixing Frankia Thr or supplemented with mineral nitrogen

Cited by 22 publications

References 46 publications

Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

The Allelopathic Effects of Sunflower and Wheat Root Exudates on Sinapis arvensis and Sinapis alba

Salt Stress Tolerance in Casuarina glauca and Its Relation with Nitrogen-Fixing Frankia Bacteria

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