Antioxidation and immune-stimulatory actions of cold alkali extracted polysaccharide fraction from Macrocybe lobayensis, a wild edible mushroom

Khatua, Somanjana; Acharya, Krishnendu

doi:10.1007/s13205-022-03317-w

Cited by 6 publications

(2 citation statements)

References 42 publications

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“…Medicinal fungi are currently studied by many ethnomycologists and medical researchers. In particular, and most importantly for modern medicine, macrofungi are a huge source of triterpene derivatives [ 7 , 8 ], polysaccharides, and polysaccharide-protein complexes with anti-cancer and immune-stimulating properties [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. Scientists are currently focusing on macrofungi, specifically a group of poroids that have antitumor effects and help boost the immune system.…”

Section: Introductionmentioning

confidence: 99%

Macrofungi as Medicinal Resources in Uzbekistan: Biodiversity, Ethnomycology, and Ethnomedicinal Practices

Gafforov,

Rašeta,

Rapior

et al. 2023

JoF

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Interest in edible and medicinal macrofungi is millennial in terms of their uses in health and food products in Central Asia, while interest in inedible and medicinal macrofungi has grown in popularity in recent years. Edible and inedible medicinal basidiomycetes were collected during field surveys from different regions of Uzbekistan. The morphological characters and similarity assessment of rDNA-Internal Transcribed Spacer sequence data were used to measure diversity and habitat associations. A number of 17 species of medicinal macrofungi of ethnomycological and medicinal interest was found associated with 23 species of trees and shrubs belonging to 11 families and 14 genera. Polyporaceae and Hymenochaetaceae were represented by the highest number of species followed by Ganodermataceae, Fomitopsidaceae, Auriculariaceae, Cerrenaceae, Grifolaceae, Phanerochaetaceae, Laetiporaceae, Schizophyllaceae, and Stereaceae. The highest number of medicinal basidiomycete species was reported in the following host genera: Acer, Betula, Celtis, Crataegus, Juglans, Juniperus, Lonicera, Malus, Morus, Platanus, Populus, Prunus, Quercus, and Salix. An updated list of edible and inedible medicinal mushrooms identified in Uzbekistan, their morphological characteristics, and phylogenetic placement are given for the first time. Information is provided on their uses in traditional and modern medicine. Their bioactive compounds and extracts can be applied as medicines, as well as food and cosmetic ingredients.

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Section: Introductionmentioning

confidence: 99%

Macrofungi as Medicinal Resources in Uzbekistan: Biodiversity, Ethnomycology, and Ethnomedicinal Practices

Gafforov,

Rašeta,

Rapior

et al. 2023

JoF

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“…Polysaccharides from plants and macrofungi have been extensively studied (Maity et al, 2020, Singha et al, 2021, Khatua and Acharya, 2022. In the last two decades, there has been a surge in the exploration of bioactive exopolysaccharides from uncanny natural sources and endophytic fungi score at the top of that novelty list (Saxena and Strobel, 2021).…”

Section: Introductionmentioning

confidence: 99%

Broad spectrum bioactivity of a novel β-glucan rich heteropolysaccharide, Pestalopine isolated from endophytic fungi Pestalotiopsis chamaeropsis CEL6

Santra

Banerjee

2023

Arch Microbiol

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A biologically potent exopolysaccharide (EPS); Pestalopine was produced by Pestalotiopsis chamaeropsis CEL6, an endophytic fungal isolate of Chloranthus elatior Sw. Pestalopine is composed of glucose, arabinose, fucose, rhamnose, and galactose in a molar ratio of nearly 10:1:2:2:4 having an Mw ∼ 3.29 x 10 5 Da. Pestalopine exhibited a radical scavenging effect and signi cantly increased antioxidant parameters (malondialdehyde, superoxide dismutase, glutathione peroxidase, reduced glutathione oxidized glutathione) in peritoneal macrophage cells in a concentration-dependent manner, with a maximum effect at 500 mg mL − 1 . Pestalopine is hepatoprotective in nature and improves the liver function pro les; -total bilirubin, direct bilirubin, hepatic enzymes-alkaline phosphatase, gammaglutamyl transferase, aspartate aminotransferase, alanine transaminase of liver brosis induced (through Thioacetamide) male Wistar rats in comparison to control. Pestalopine-fed rats are reported to have higher counts of bene cial Lactobacillus sp. Present ndings suggest that Pestalopine may have promise as a non-toxic exogenous antioxidant with hepatoprotective and probiotic e cacies. Contribution to the eldSecondary metabolites synthesized by endophytic fungi hold numerous pharmaceutical potentialities.Especially exopolysaccharides produced in culture broth are majorly antioxidative in action. In the current situation, there is a huge scarcity of potent antioxidants that can replace contemporary synthetic ones which hold numerous detrimental side effects. Natural products are always the rst choice to replace the existing ones and other than plant sources, novel metabolites from potent endophytic fungi are acting as prominent sources nowadays. Here an effort has been made to explore secondary metabolites from fungal endophytes of medicinal plants for the production of potent antioxidants. In the present investigation, β-glucan rich heteropolysaccharide (named Pestalopine) produced by an endophytic fungi Pestalotiopsis chamaeropsis CEL6 (isolated from Chloranthus elatior Sw.) exhibited in-vitro free radical scavenging activity, antioxidative activity, and in-vivo hepatoprotective activity. Also, Pestalopine modulated gut microbial ora of treated Male Wistar rats. Investigational outcomes shades light on the immense health bene ts of endophytic fungal secondary metabolites, mainly macromolecules like exopolysaccharides. The present outcome holds future potential for the commercialization of Pestalopine as a major in-vitro and in-vivo antioxidant.

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Chemical Characterization and Biological Functions of Hot Alkali-Soluble Crude Polysaccharide from the Water-Insoluble Residue of Macrocybe lobayensis (Agaricomycetes) Fruit Bodies

Khatua

ACHARYA

2023

Int J Med Mushrooms

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<i>Macrocybe lobayensis</i> owe popularity in several traditional cultures not only for delectable taste but also for its nutraceutical profile conveying great health benefits. Previous investigations have enumerated several bioactivities of the valuable mushroom such as antioxidant, anti-ageing, immune-modulation, and anti-tumor properties where polysaccharides played the key role. Macrofungi polymers are generally isolated by the conventional hot water process discarding the residue which still contains plenty of therapeutic components. The present study thus aimed to re-use such leftover of the edible macrofungus by immersing it in NaOH solution at high temperature supporting circular economy. Consequently a polysaccharide fraction, namely ML-HAP, was isolated that was found to be consisted of a homogenous heteropolysaccharide with molecular weight of ~ 128 kDa and β-glucan as the chief ingredient as evident by spectroscopy, gel-permeation chromatography, high performance thin-layer chromatography, and Fourier transform infrared. Antioxidant activity assays revealed that the macromolecules possess good radical scavenging, metal ion binding and reducing power. Nevertheless, strong immune-potentiation was also recorded as the extract triggered murine macrophage cell viability, pinocytosis, nitric oxide production and morpho-dynamics within 24 h where the best effect was executed at the level of 100 µg/mL. Altogether, the polysaccharides extracted from <i>M. lobayensis</i> exhibited a potent application prospect in functional food, pharmaceutical, nutraceutical and health care industries that could raise economic value of the underexplored mushroom.

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Antioxidation and immune-stimulatory actions of cold alkali extracted polysaccharide fraction from Macrocybe lobayensis, a wild edible mushroom

Cited by 6 publications

References 42 publications

Macrofungi as Medicinal Resources in Uzbekistan: Biodiversity, Ethnomycology, and Ethnomedicinal Practices

Macrofungi as Medicinal Resources in Uzbekistan: Biodiversity, Ethnomycology, and Ethnomedicinal Practices

Broad spectrum bioactivity of a novel β-glucan rich heteropolysaccharide, Pestalopine isolated from endophytic fungi Pestalotiopsis chamaeropsis CEL6

Chemical Characterization and Biological Functions of Hot Alkali-Soluble Crude Polysaccharide from the Water-Insoluble Residue of Macrocybe lobayensis (Agaricomycetes) Fruit Bodies

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