Antioxidants profile of Momordica charantia fruit extract analyzed using LC-MS-QTOF-based metabolomics

Perumal, Vikneswari; Khatib, Alfi; Ahmed, Qamar Uddin; Uzir, Bisha Fathamah; Abas, Faridah; Murugesu, Suganya; Saiman, Mohd Zuwairi; Primaharinastiti, Riesta; El‐Seedi, Hesham R.

doi:10.1016/j.fochms.2021.100012

Cited by 18 publications

(11 citation statements)

References 41 publications

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“…DPPH and FRAP assays work well when the organic medium is used as a solvent to prepare solutions for assays compared to water. However, as far as ABTS is concerned, this assay performs well in aqueous medium . The boiling treatment on the extracts also increases inhibiting activity due to the extraction of cell-bound antioxidant compounds compared to a room-temperature water extract .…”

Section: Resultsmentioning

confidence: 99%

Formulation of Whole Wheat Bitter Gourd-Supplemented Cookies for Improving Blood Glycemic Levels: Insights of Inhibition Assays and Antioxidant Activities

Khan,

Ahmed,

Maghaydah

et al. 2024

ACS Food Sci. Technol.

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Mechanical lifestyles have increased the incidence of diabetes over the past decade, making it a significant, rapidly growing, and degenerative disease all around the globe. The study aimed to evaluate the efficacy of the hypoglycemic effect of bitter gourd (BG)-supplemented whole wheat cookies in managing blood glucose levels. 1−3% (w/w) BG powder was supplemented into cookies. The cookies showed a significant positive relation (p < 0.05) between supplementation of BG, radical scavenging potential, ferric-reducing ability, presence of phenols, and inhibition of α-amylase and α-glucosidase enzymes. The one-week cookies' storage study showed insignificant differences in the alteration of antioxidant and antidiabetic activity of BG cookies, showing its stability at room temperature. Incorporating BG into cookies at different inclusion levels showed highly significant results (p < 0.05) for all treatments of α-amylase and α-glucosidase inhibition. 1% (w/w) BG cookies showed promising sensory evaluation results and better overall acceptability.

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Section: Resultsmentioning

confidence: 99%

Formulation of Whole Wheat Bitter Gourd-Supplemented Cookies for Improving Blood Glycemic Levels: Insights of Inhibition Assays and Antioxidant Activities

Khan,

Ahmed,

Maghaydah

et al. 2024

ACS Food Sci. Technol.

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“…The DPPH method was conducted by following the guidelines described in the literature 27 with slight adjustments. A 1.0 mL of DPPH 0.2 mM was added to 1.0 mL of extracts at different percentages as a reaction solution.…”

Section: Dpph Activitymentioning

confidence: 99%

Phytochemical Screening, Total Phenolic, Reducing Sugar Contents, and Antioxidant Activities of Gelidium spinosum (S.G. Gmelin) P.C. Silva

2023

TJNPR

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Gelidium spinosum is edible red seaweed with high economic values and potential pharmacological activities. This research aimed to evaluate phytochemicals, total phenolic content, reducing sugar content, and antioxidant properties of Gelidium spinosum methanolaqueous extracts. Maceration with different solvent ratios of methanol-water was employed to afford various crude extracts. The standard procedures of preliminary phytoconstituents determination were employed to screen the presence of various phytochemicals. Phenolic and reducing sugar contents were determined using Folin-Cioucalteu and 3,5-dinitro salicylic acid methods. The antioxidant activities of seaweed extracts were determined through DPPH and reducing power assays. The 100% methanol extract of G. spinosum was found to be rich in alkaloids, flavonoids, glycosides, polyphenols, proteins, reducing sugar, saponins, steroids and tannins. The aqueous extract of G. spinosum contained flavonoids, glycosides, polyphenols, reducing sugars, saponins and tannins at a moderate level. The total phenolic content range was 6.43 to 49.78 mg EGA/g extract. The highest reducing sugar content was shown by 100% methanol extract (1278.20 ± 21.25 mg GE/g extract). The highest antioxidant activities were found in 100% methanol extract for reducing power assay, and 75% methanol extract of G. spinosum for DPPH method. There was a positive correlation between reducing sugar, total phenolic contents and antioxidant activities. Results further confirmed the potential use of red seaweed in various ailments, however, should further be confirmed through more appropriate similar studies.

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“…The diverse class of metabolite also showed the presence of yuccaol C a phenolic compound that the showed the highest concentration in MBI and lowest in BCPI. The previous studies by Pecio et al (2019) showed the neuroprotective effect of yuccaol C. The study by Perumal et al (2021) reported the antioxidant activity of goyaglycoside G. The study also revealed that antioxidants are essential in the prevention of diseases like diabetes mellitus (DM), coronary heart disease, cancer, Alzheimer's disease and hepatotoxicity that have serious health consequences by inhibition reactive oxygen species (ROS). Lipids and their derivatives (26) were also found in the protein isolates.…”

Section: Metabolite Profiling Using Uhplc-qtof-imsmentioning

confidence: 99%

Insights into the non‐targeted metabolomic profile, rheological, functional and structural characteristics of rice bran and pulse protein isolates

Kumar

Joshi

Awasthi

et al. 2023

Int J of Food Sci Tech

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SummaryRice bran protein isolates and seven legume protein isolates (moong bean, green pea, white pea, black chickpea and white chickpea, soya bean and lentil) were evaluated for functional, structural and metabolomic properties. Rice bran protein isolates (RBI) had higher solubility, foaming, oil absorption capacity and water absorption capacity but lower denaturing temperature compared to legume proteins. The functionality of proteins isolates impacted significantly with structural characteristics. The β‐sheet, α‐helix, β‐turn and anti‐parallel β‐sheets proportions were evaluated by FTIR. RBI revealed lower molecular weight, less rigid conformational structure and lower proportion of β‐sheet. The evaluation of untargeted metabolomics compounds was determined by ultraperformance liquid chromatography quadruple time‐of‐flight ion mobility mass spectrometry (UHPLC‐QTOF‐IMS), evaluated using PCA and presented as a heatmap. Analysis revealed 46 metabolites including sugars, amino acids, lipids, terpenoid, phenolics, saponins and their derivatives. Significant differences in selected compounds from 126 identified metabolites among protein isolates were observed. This work provides new insights into metabolite distribution among protein isolates and also presents future implementations for applications in food and nutraceuticals.

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Antioxidants profile of Momordica charantia fruit extract analyzed using LC-MS-QTOF-based metabolomics

Cited by 18 publications

References 41 publications

Formulation of Whole Wheat Bitter Gourd-Supplemented Cookies for Improving Blood Glycemic Levels: Insights of Inhibition Assays and Antioxidant Activities

Formulation of Whole Wheat Bitter Gourd-Supplemented Cookies for Improving Blood Glycemic Levels: Insights of Inhibition Assays and Antioxidant Activities

Phytochemical Screening, Total Phenolic, Reducing Sugar Contents, and Antioxidant Activities of Gelidium spinosum (S.G. Gmelin) P.C. Silva

Insights into the non‐targeted metabolomic profile, rheological, functional and structural characteristics of rice bran and pulse protein isolates

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