Antioxidants Prevent the Cellular Deficit Produced in Response to Burn Injury

Lalonde, Cheryl; Nayak, Uma; Hennigan, John; Demling, Robert H.

doi:10.1097/00004630-199609000-00002

Cited by 33 publications

(9 citation statements)

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“…We do not know what mechanisms were responsible for these observations. Cigarette smoke contains superoxide, catachols, and hydroquinones, as well as nitric oxide (18,31). The effects of superoxide on IRP binding activity are inconclusive because the methods used to impose oxidative stress influence the experimental results.…”

Section: Discussionmentioning

confidence: 99%

“…An estimated 1.2 g of iron can be delivered from a pack of unfiltered cigarettes, a portion of which remains in the respiratory cells (49). Iron, delivered from smoke, can induce oxidative stress in lung tissues in vivo with adverse consequences (31). Lung cells also are unique in that they are routinely exposed to changes in the levels of oxygen and oxygen tension.…”

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confidence: 99%

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Effects of sham air and cigarette smoke on A549 lung cells: implications for iron-mediated oxidative damage

Mayo

Kohlhepp

Zhang

et al. 2004

American Journal of Physiology-Lung Cellular and Molecular Phys

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Inhalation of airborne pollution particles that contain iron can result in a variety of detrimental changes to lung cells and tissues. The lung iron burden can be substantially increased by exposure to cigarette smoke, and cigarette smoke contains iron particulates, as well as several environmental toxins, that could influence intracellular iron status. We are interested in the effects of environmental contaminants on intracellular iron metabolism. We initiated our studies using lung A549 type II epithelial cells as a model, and we evaluated the effects of iron dose and smoke treatment on several parameters of intracellular iron metabolism. We show that iron at a physiological dose stimulates ferritin synthesis without altering the transferrin receptor (TfR) mRNA levels of these cells. This is mediated primarily by a reduction of iron regulatory protein 2. Higher doses of iron reduce iron regulatory protein-1 binding activity and are accompanied by a reduction in TfR mRNA. Thus, for A549 cells, different mechanisms influencing IRP-IRE interaction allow ferritin translation in the presence of TfR mRNA to provide for iron needs and yet prevent excessive iron uptake. More importantly, we report that smoke treatment diminishes ferritin levels and increases TfR mRNA of A549 cells. Ferritin serves as a cytoprotective agent against oxidative stress. These data suggest that exposure of lung cells to low levels of smoke as are present in environmental pollutants could result in reduced cytoprotection by ferritin at a time when iron uptake is sustained, thus enhancing the possibility of lung damage by iron-mediated oxidative stress.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effects of sham air and cigarette smoke on A549 lung cells: implications for iron-mediated oxidative damage

Mayo

Kohlhepp

Zhang

et al. 2004

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Antioxidants are decreased in multiple tissues post-burn injury (59). Supplements of both ascorbic acid and glutathione attenuate the decrease in hepatic ATP production following burn trauma (58,60). Others have found that high doses of ascorbic acid decreased lipid peroxidation products (70).…”

Section: Future Potential Treatments For Posttrauma Insulin Resistancementioning

confidence: 99%

Postburn trauma insulin resistance and fat metabolism

Cree

Wolfe²

2008

American Journal of Physiology-Endocrinology and Metabolism

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“…REACTIVE SPECIES OF OXYGEN (ROS) have been implicated as being important mediators in a variety of pathological conditions, including burns (20), various forms of ischemia-reperfusion (I/R) injury (8,15,16,26), and hemorrhagic shock (9,10,17,32 Pyruvate, a key intermediate in cellular metabolism, is an effective scavenger of ROS. In a reaction characteristic of ␣-keto carboxylic acids in general, pyruvic acid (the simplest member of this class of compounds) rapidly undergoes nonenzymatic decarboxylation in the presence of H 2 O 2 to form acetate, carbon dioxide, and water (5,33).…”

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confidence: 99%

Ethyl pyruvate modulates inflammatory gene expression in mice subjected to hemorrhagic shock

Yang¹,

Gallo²,

Baust³

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

155

143

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Administration of pyruvate, an effective scavenger of reactive oxygen species, has been shown to be salutary in numerous models of redox-mediated tissue or organ injury. Pyruvate, however, is unstable in solution and, hence, is not attractive for development as a therapeutic agent. Herein, ethyl pyruvate, which is thought to be more stable than the parent compound, was formulated in a calcium-containing balanced salt solution [Ringer ethyl pyruvate solution (REPS)] and evaluated in a murine model of hemorrhagic shock and resuscitation (HS/R). Resuscitation with REPS instead of Ringer lactate solution (RLS) significantly improved survival at 24 h and abrogated bacterial translocation to mesenteric lymph nodes and the development of increased ileal mucosal permeability to FITC-labeled dextran (4,000 Da) at 4 h. Mice treated with REPS instead of RLS also had lower circulating levels of alanine aminotransferase at 4 h. Treatment with REPS instead of RLS decreased activation of nuclear factor-κB in liver and colonic mucosa after HS/R and also decreased the expression of inducible nitric oxide synthase, tumor necrosis factor, cyclooxygenase-2, and interleukin-6 mRNA in liver, ileal mucosa, and/or colonic mucosa. These data support the view that resuscitation with REPS modulates the inflammatory response and decreases hepatocellular and gut mucosal injury in mice subjected to HS/R.

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Antioxidants Prevent the Cellular Deficit Produced in Response to Burn Injury

Cited by 33 publications

References 0 publications

Effects of sham air and cigarette smoke on A549 lung cells: implications for iron-mediated oxidative damage

Effects of sham air and cigarette smoke on A549 lung cells: implications for iron-mediated oxidative damage

Postburn trauma insulin resistance and fat metabolism

Ethyl pyruvate modulates inflammatory gene expression in mice subjected to hemorrhagic shock

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