Antioxidants, anti-inflammatory, and antidiabetic effects of the aqueous extracts from Glycine species and its bioactive compounds

Abstract

BackgroundThe aim of this study was to examine the possible antioxidant, anti-inflammatory, and antidiabetic effects of the aqueous extracts from three Glycine species. In HPLC analysis, the chromatograms of three Glycine species were established. Flavonoid-related compounds might be important bioactive compounds in Glycine species.ResultsThe results showed that the aqueous extract of Glycine tabacina (AGTa) had the strongest antioxidant activity compared with the other Glycine species extracts. We also found that AGTa had higher contents of total polyphenol compounds and flavonoids than the other extracts. We also have investigated the anti-inflammatory effects of the three Glycine species using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) ex vivo. When RAW264.7 macrophages were treated with different concentrations of three Glycine species together with LPS, a significant concentration-dependent inhibition of NO production was detected. The aqueous extract of Glycine max (AGM) had the strongest anti-inflammatory activity in comparison with the other Glycine species extracts. Western blotting revealed that three Glycine species blocked protein expression of iNOS and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW264.7 macrophages, significantly. The antidiabetic activities of the three Glycine species were studied in vitro using α-glucosidase and aldose reductase (AR) inhibitory methods. AGTa had the highest inhibitory activities on α-glucosidase and aldose reductase, with IC50 of 188.1 and 126.42 μg/mL, respectively. The bioactive compounds, genistein and daidzein, had high inhibitory activities on antioxidant, anti-inflammatory, α-glucosidase and aldose reductase.ConclusionsThese results suggest that Glycine species might be a good resource for future development of antioxidant, anti-inflammatory and antidiabetic heath foods.

Exploring protective effect of Glycine tabacina aqueous extract against nephrotic syndrome by network pharmacology and experimental verification

“…Flavonoids which are abundant in various medicinal herbs and food sources are well-known for their protective roles in renal disorders such as diabetic nephropathy, glomerulonephritis and kidney insufficiency [49]. High total polyphenols content and total flavonoids content have been reported in aqueous extract of G. tabacina and four common dietary flavonoids (daidzein, genistein, daidzin and genistin) were identified by HPLC analysis [13]. In the present study, we revealed that six flavonoids, i.e.…”

“…Benth, a well-known folk medicine and Chinese herbal medicine, is a leguminous plant which is mainly distributed in Australia, the Ryukyu Islands, southern China, and some South Pacific Islands. Its roots have been utilized for the treatment of osteoporosis, nephritis, rheumatism and menopausal syndrome in folk medicine [13,14]. In addition, G. tabacina is also famous as one of the sources of the folk medicine 'I-Tiao-Gung' and serves as a herb tea in Taiwan [13].…”

“…Its roots have been utilized for the treatment of osteoporosis, nephritis, rheumatism and menopausal syndrome in folk medicine [13,14]. In addition, G. tabacina is also famous as one of the sources of the folk medicine 'I-Tiao-Gung' and serves as a herb tea in Taiwan [13]. However, few of phytochemical and pharmacological studies have been conducted and reported on this species [13,15].…”

“…In addition, G. tabacina is also famous as one of the sources of the folk medicine 'I-Tiao-Gung' and serves as a herb tea in Taiwan [13]. However, few of phytochemical and pharmacological studies have been conducted and reported on this species [13,15]. In vitro anti-inflammatory, anti-arthritic, antioxidant and antidiabetic activities of its extract have been reported [13,16,17].…”

“…However, few of phytochemical and pharmacological studies have been conducted and reported on this species [13,15]. In vitro anti-inflammatory, anti-arthritic, antioxidant and antidiabetic activities of its extract have been reported [13,16,17]. Nevertheless, the therapeutic effect of G. tabacina aqueous extract (GATE) on NS is completely unknown.…”