Antioxidant Systems as a Response to Midgut Cellular of Bombyx mori Lineu, 1758 (Lepidoptera: Bombycidae) Infection for Baculoviruses

Vessaro-Silva, S A; Neto, Marcílio Hubner Miranda; Brancalhão, Rose Meire Costa; Ribeiro, Lucinéia de Fátima Chasko; Guimarães, Ana Tereza Bittencout; Oliveira, Camila Maciel de

doi:10.1093/jee/toz009

Cited by 7 publications

(4 citation statements)

References 52 publications

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“…The results were consistent with those in GO and KEGG. Some antioxidant-related proteins were found in the tryptophan metabolic pathway, such as catalase, which acts as a barrier against viral infections [47]. In Aedes aegypti, viral resistance is closely related to catalase, which is upregulated after hemolymph intake to protect the body from viruses in the hemolymph [48].…”

Section: Discussionmentioning

confidence: 99%

“…In Aedes aegypti, viral resistance is closely related to catalase, which is upregulated after hemolymph intake to protect the body from viruses in the hemolymph [48]. Catalase was also studied in BmNPV, but it cannot stop the infection from occurring since a coevolutionary bond is maintained between the virus and the host in B. mori [47]. Therefore, catalase should be a related gene that affects the resistance of silkworms.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains

et al. 2021

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Bombyx mori nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although Autographa californica nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of BmTex261 in viral infection was analysed. The BmTex261 showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene lef3 after overexpression of BmTex261 in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains

et al. 2021

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“…Interference on apoptosis, cytoskeleton, protein folding, metabolism, and ribosome structure was detected since 6 hpi. Regarding apoptosis, a down-regulation of the PRX gene was detected, which is believed to participate with proteins related to redox homeostasis, which may have an antioxidant effect and oxidoreductase activity (Vessaro-Silva et al, 2019). It is also believed to participate in phosphatidylinositol 3 kinase (PI3K) signaling.…”

Section: The Apoptotic System Implicationsmentioning

confidence: 99%

Transcriptomics and interactomics during the primary infection of an SfNPV baculovirus on Spodoptera frugiperda larvae

Rangel-Núñez,

Ibarra,

Del Rincón-Castro

2023

Front. Cell. Infect. Microbiol.

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The fall armyworm (FAW), Spodoptera frugiperda, has been the most devastating pest of corn as well as of other crops in America, and more recently in Africa and Asia. The development of resistance to chemical insecticides led the search for environmentally friendly biological alternatives such as baculoviruses. This study focuses on the primary infection of the baculovirus SfNPV-Ar in the FAW’s midgut epithelium, by analyzing the differential expression of transcripts in excised midguts at 6, 12, and 24 h post-infection (hpi), and predicted their interactions. Interaction of viral factors with the infected midgut tissue could alters various cellular processes, such as the apoptotic system due to the up-regulation observed of FABP at 6 hpi and of HSP90 at 24 hpi, along with the down-regulated PRX at 6 hpi and FABP transcripts between 12 and 24 hpi. Changes in transcript regulation could affect the cellular architecture of infected cells due to up-regulation of ARP 2/3 at 6 and 12 hpi, followed by down-regulation at 24 hpi. In relation to protein folding proteins, HSP90 was up-regulated at 24 hpi and PDI was down-regulated between 6 and 12 hpi. With respect to metabolism and cellular transport, AcilBP and ATPS0 were up regulated at 6 hpi and 12 hpi, respectively. In reference to transcription and translation up-regulation of RPL11 at 6 hpi and of FPN32 and RPL19 at 24 hpi was detected, as well as the down-regulation of RPL19 at 6 hpi, of PDI and RPL7 at 12 hpi, and of FABP at 24 hpi. In conclusion, gene regulation induced by viral infection could be related to the cytoskeleton and cellular metabolism as well as to oxidative stress, apoptosis, protein folding, translation, and ribosomal structure. The results presented in this work are an approach to understanding how the virus takes control of the general metabolism of the insect host during the primary infection period.

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“…In the larval stage, B. mori is particularly susceptible to BmNPV, and infection occurs mainly orally, by ingestion of food contaminated with viral polyhedra (Ribeiro et al, 2009a;Vessaro-Silva et al, 2019). BmNPV is polyorganotrophic, and several tissues have already been recognized as targets (Torquato et al, 2006a;Brancalhão et al, 2009;Ribeiro et al, 2009a;Baggio, et al, 2014;Vessaro-Silva et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Research Article Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) larva integument infected by Bombyx mori nucleopolyhedrovirus

Oro¹,

Brancalhão²,

Britta³

et al. 2021

Genet. Mol. Res.

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Bombyx mori nucleopolyhedrovirus (BmNPV) is an entomopathogenic virus of the family Baculoviridae that causes disease in the silkworm, Bombyx mori, leading to a series of metabolic disorders that severely affect silk production. The main external sign of BmNPV infection can be observed in the integument of infected caterpillars, which is an important diagnostic indicator that can help direct control measures to prevent viral dispersion in rearing facilities. We analyzed the integument of B. mori caterpillars infected with BmNPV in a cytopathological study of epidermal cells and cuticle, to help identify signs of infection. Fifth instar larvae were inoculated with viral suspension and integument segments were processed for light microscopy and transmission electron microscopy. Signs of infection were monitored daily until cocooning. Cytopathology of epidermal cells showed signs of baculovirus infection, leading to cytolysis and release of viral polyhedra into the hemocoel, similar to what occurs in other BmNPV target cells. Considerable modifications were observed in the apical region of the epidermal cells, with disarrangement and involution of the microvilli, and loss of contact of the plasma membrane plaques ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 20 (2): gmr18758 A.L. Oro et al. 2with the endocuticle. Plasma membrane plaques are involved in the transport of precursors for chitin synthesis and assembly of chitin myofibrils. The disorganization of the integument of silkworms infected with BmNPV shows the fragility of this organ, which easily ruptures due to infection, leading to the release of BmNPV polyhedra to the external environment, perpetuating the infection.

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Antioxidant Systems as a Response to Midgut Cellular of Bombyx mori Lineu, 1758 (Lepidoptera: Bombycidae) Infection for Baculoviruses

Cited by 7 publications

References 52 publications

Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains

Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains

Transcriptomics and interactomics during the primary infection of an SfNPV baculovirus on Spodoptera frugiperda larvae

Research Article Cytopathology of <i>Bombyx</i> <i>mori</i> (Lepidoptera: Bombycidae) larva integument infected by <i>Bombyx</i> <i>mori</i> nucleopolyhedrovirus

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