Antioxidant status and thigh meat quality of broiler chickens fed diet supplemented with α-tocopherolacetate, pomegranate pomace and pomegranate pomace extract

Abstract

The effects of supplementation with a-tocopherol acetate (a-Toc), pomegranate pomace extract (PPE) and pomegranate pomace (PP) into chicken feed on antioxidant status, oxidation susceptibility and quality of the thigh meat during refrigeration were investigated. During six weeks broiler chickens were fed eight dietary treatments, which included: control diet, a-tocopherol diet (200 mg kg À1), PPE diets (0.1, 0.2 and 0.3 g kg À1) and PP diets (1, 2 and 3 g kg À1). Feed efficiency was significantly improved by supplementing chickens fed diet with 0.2 g kg À1 PPE. Long chain polyunsaturated fatty acids (LC PUFA) n-3 level was higher in the thigh of broilers fed with a-Toc and PPE diets (except 0.1 g kg À1) than in chickens fed control and PP diets (p < .05). Total phenolic content, lipid peroxidation level and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity in the thigh meat were significantly improved when chickens were fed diets containing a-Toc and PPE (p < .05). Supplementation of different antioxidant preparations into diets had no influence on plasma superoxide dismutase and glutathione peroxidase activities, in contrast, serum lipid peroxidation level was reduced in chickens fed diets supplemented with PPE (except 0.1 g kg À1) and a-Toc. In conclusion, the broiler thigh meat may be successfully enriched with LC PUFA n-3 and its antioxidant potential and quality characteristics may be improved by supplementing diets with 0.2 and 0.3 g kg À1 PPE. Moreover, the antioxidant potential of PPE supplementation was equal to that of a-tocopherol acetate in refrigerated meat.

Effect of pomegranate (Punica granatum L) peel powder meal dietary supplementation on antioxidant status and quality of breast meat in broilers

“…They either directly scavenge for free radical species, or indirectly improve the cell's innate defense mechanisms, by activating the activities of antioxidant enzymes such as SOD, CAT, etc. Superoxide dismutase (SOD) and catalase (CAT) are key hepatic antioxidant enzymes that catalytically scavenge free radicals and other ROS, thereby conferring endogenous protection on biological systems against oxidative stress ( Saleh et al., 2017a ). These endogenous antioxidant enzymes help to maintain the health of poultry birds, as well as the physiology of antioxidative systems ( Ajuwon et al., 2016 ).…”

“…Over the years, the extracts of pomegranate fruit peel have had positive effects on the meat quality and antioxidant capacity of broiler meat ( Chandralekha et al., 2012 ; Saleh et al., 2017a ; b ; Sarica and Urkmez, 2018 ; Kishawy et al., 2019 ; Sharifian et al., 2019 ). Vitamin E (α-tocopherol acetate) is a fat-soluble radical scavenging supplement that can also delay lipid oxidative processes in meat and its associated products ( Karami et al., 2010 ; Li and Liu, 2012 ).…”

Green Tea and Pomegranate Extract Administered During Critical Moments of the Production Cycle Improves Blood Antiradical Activity and Alters Cecal Microbial Ecology of Broiler Chickens

“…Recently, phytobiotics gained increasing attention as a replacement for antimicrobial growth promoters to enhance growth performance and improve animal health [42,43]. The positive effects of phytobiotics have been associated with high polyphenolic content, which can counteract the effect of free radical generation [15], and their ability to modulate gut microflora composition, leading to increased performance [14,22].…”

“…In the present study, the lack of positive results as expected might be due to the administration route, the dosage applied, or the duration of supplementation. Generally, supplementation of poultry with green tea and pomegranate extracts was shown to improve broiler productivity [20][21][22]. However, nearly all studies reporting positive effects on growth performance administered the compounds in the feed and for the entire rearing period.…”

“…Among phytobiotics, green tea and pomegranate extracts have been shown to improve broiler productivity and antioxidant status [20][21][22], as well as modulate the intestinal microflora [20,23]. Green tea (Camelia sinensis) has been widely studied in humans and animals due to its numerous biofunctional properties, including antioxidant, antiviral and anticoccidial activity [24,25].…”