Antioxidant Sensors Based on DNA-Modified Electrodes

Abstract

TiO 2 /ITO modified electrodes were developed to quantitatively photo-oxidise adsorbed ds-DNA and to study the effect of antioxidants as ds-DNA protecting agents. TiO 2 films are used for efficient ds-DNA immobilization, for ds-DNA oxidation through photogenerated hydroxyl radicals and as electrode for amperometric sensing. The films, prepared by a sol-gel process, are deposited on ITO glass electrodes. Damages occurring after ds-DNA oxidation by ROS, are detected by adding MB as an intercalant probe and by monitoring the electrochemical reduction current of the intercalated redox probe. The MB electrochemical signal is found to be sensitive enough to monitor ds-DNA structure changes and the electrochemical sensor has been applied to the evaluation of the antioxidant properties of glutathione and gallic acid.

Electrochemical Sensors

“…Girault's group described a DNA modified electrode sensor for the detection of antioxidants using a TiO 2 /ITO modified electrode. 199 Double stranded DNA was efficiently immobilized on the TiO 2 film. The dsDNA was oxidized through photogenerated hydroxyl radicals.…”

Electrochemical Pseudo‐Titration of Water‐Soluble Antioxidants

“…Electrochemical-based sensors have been used for measuring the AO activity due to their simple, fast and reliable analytical performance on biological and food samples [29][30][31][32][33][34][35][36]. Moreover, electrochemical strategies can also take into account the complete range of chemical and biochemical interactions between all present species, including recycling or auto-oxidation processes.…”

Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage

“…For instance, DNA damage was investigated with electrochemical and electrochemiluminescent sensors and sensor arrays by a number of research groups [10][11][12][13][14][15]. We also developed a photoelectrochemical DNA sensor for the rapid detection of chemical-induced DNA damage [16][17][18][19].…”