Antioxidant responses and plasma biochemical characteristics in the freshwater rainbow trout, Oncorhynchus mykiss, after acute exposure to the fungicide propiconazole

Abstract

AbstrAct:In this study, the toxic effects of PCZ, a triazole fungicide present in aquatic environment, were studied in rainbow trout, Oncorhynchus mykiss, by an acute toxicity test. Compared to the control group, fish exposed to PCZ (96-h-LC50 , 5.04 mg/l) showed significantly higher (P < 0.05) plasma NH 3 and GLU concentration and the activities of plasma enzymes including CK, ALT, AST, LDH, but the TP content was not significantly different (P > 0.05). The oxidative stress indices (levels of LPO and CP) of brain and muscle in the experimental group were higher compared to the control group, especially for a significant change (P < 0.05) in the brain. SOD, CAT, GPx and GR activity in the brain of experimental groups was significantly lower (P < 0.05), however, an opposite tendency was found out in muscle. In addition, there are significant correlations between TBARS and CAT, TBARS and GPx, CP, and CAT, GR, and GPx in the fish brain. Thus, PCZ exposure changed the oxidative stress indices and plasma characteristics, and these changes may be used as potential bioindicators of the exposure and effect of PCZ in the controlled experiment. The use in monitoring of PCZ exposure under natural field conditions is possible, but it needs further investigations.Keywords: triazole fungicide; fish; oxidative damage; blood parameters List of abbreviations PCZ = propiconazole; rOs = reactive oxygen species; LPO = lipid peroxidation; cP = carbonyl protein; sOD = superoxide dismutase; cAt = catalase; GPx = glutathione peroxidase; Gr = glutathione reductase; tbArs = thiobarbituric acid-reactive substances; DMsO = dimethyl sulphoxide; GssG = oxidized glutathione; tcA = trichloroacetic acid; bHt = butylated hydroxytoluene; DNPH = 2,4-di-nitrophenylhydrazine; EDtA = ethylenediamine-tetraacetic acid; NADPH = nicotinamide adenine dinucleotide phosphate; HUFA = highly unsaturated fatty acids; NH 3 = ammonia; GLU = glucose; tP = total proteins; cK = creatine kinase; LDH = lactate dehydrogenase; ALt = alanine aminotransferase; Ast = aspartate aminotransferase

Effects of sublethal zinc ions and acute crude oil exposure on serum enzymes activity of Carassius gibelio

“…The current research is in agreement with the finding obtained by Naveed et al (2011) in Channa punctatus who noticed that frequent exposure to heavy elements led to augmented activities of both aminotransferases advised greater than before transamination operation. As well as, complemented with the previous research of Li et al (2011) who studied the freshwater rainbow trout (Oncorhynchus mykiss) which have been exposed to toxic fungal pesticides, who found that concentration level of AST and ALT in experimental samples were considerably higher than those found in the control samples. Another study by Zikic et al (2001) on Carassius auratus gibelio exposed to Cd element ions they proved that high activity in plasma AST and ALT and this rise in activity of enzymes could result from impairment in physiological and anatomical characteristics for tissues.…”

Short-term exposure to L-type calcium channel blocker, verapamil, alters the expression pattern of calcium-binding proteins in the brain of goldfish, Carassius auratus

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Lipidemic Properties of Sorghum vulgare Leaf Sheath on Oxidative Markers and Heart Function Enzymes of Dyslipidemic Wistar-Albino Rats

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