Antioxidant responses and degradation of two antibiotic contaminants in Microcystis aeruginosa

Liu, Ying; Guan, Yuntao; Gao, Baoyu; Yue, Qinyan

doi:10.1016/j.ecoenv.2012.09.004

Cited by 123 publications

(40 citation statements)

References 63 publications

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“…Besides, cyanobacteria could degrade several antibiotic contaminants including minocycline [29], erythromycin, and ibuprofen [30], and the degradation products of these antibiotics were found to promote the growth of cyanobacteria. Degradation of amoxicillin could also be achieved in M. aeruginosa [31]. Degradation products of amoxicillin may be another explanation for the promoted algal growth, which should be verified by further study.…”

Section: Discussionmentioning

confidence: 97%

Hormesis Effects of Amoxicillin on Growth and Cellular Biosynthesis of Microcystis aeruginosa at Different Nitrogen Levels

et al. 2014

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Coexisting antibiotic contaminants have potential to regulate cyanobacterial bloom, and the regulation is likely affected by nitrogen supply. A typical cyanobaterium Microcystis aeruginosa was cultured with 0.05-50 mg L(-1) of nitrogen and exposed to 100-600 ng L(-1) of amoxicillin for 7 days. Algal growth was not significantly (p > 0.05) affected by amoxicillin at the lowest nitrogen level of 0.05 mg L(-1), stimulated by 600 ng L(-1) of amoxicillin at a moderate nitrogen level of 0.5 mg L(-1) and enhanced by 100-600 ng L(-1) of amoxicillin at higher nitrogen levels of 5-50 mg L(-1). Amoxicillin affected chlorophyll-a, psbA gene, and rbcL gene in a similar manner as algal growth, suggesting a regulation of algal growth via the photosynthesis system. At each nitrogen level, synthesis of protein and polysaccharides as well as production and release of microcystins (MCs) increased in response to environmental stress caused by amoxicillin. Expression of ntcA and mcyB showed a positive correlation with the total content of MCs under exposure to amoxicillin at nitrogen levels of 0.05-50 mg L(-1). Nitrogen and amoxicillin significantly (p < 0.05) interact with each other on the regulation of algal growth, synthesis of chlorophyll-a, production and release of MCs, and expression of ntcA and mcyB. The nitrogen-dependent stimulation effect of coexisting amoxicillin contaminant on M. aeruginosa bloom should be fully considered during the combined pollution control of M. aeruginosa and amoxicillin.

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Section: Discussionmentioning

confidence: 97%

Hormesis Effects of Amoxicillin on Growth and Cellular Biosynthesis of Microcystis aeruginosa at Different Nitrogen Levels

et al. 2014

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“…The gradually decrease in SOD activity at higher treatment levels suggests that TCS can inhibit detoxification of O 2 − by M. aeruginosa cells. H 2 O 2 generated during the conversion of O 2 − can be further eliminated by CAT and POD (Liu et al, 2012). CAT could not be detected while no significant difference in POD activity was found between the treatments and control (p ≥ 0.379), suggesting that POD is not sensitive to TCS exposure.…”

Section: Effects Of Tcs On Antioxidant Activities and Microcystin Promentioning

confidence: 92%

“…Environmental stressors such as pollutants, allelochemicals, and radiations can affect the antioxidant activities of cyanobacteria (Choudhary et al, 2007;Hong et al, 2008;Liu et al, 2012). During the experiment, only SOD, POD and GSH were found above the limits of detection of the commercial reagent kits (Fig.…”

Section: Effects Of Tcs On Antioxidant Activities and Microcystin Promentioning

confidence: 99%

Interactions between the antimicrobial agent triclosan and the bloom-forming cyanobacteria Microcystis aeruginosa

Huang

Cheng

et al. 2016

Aquatic Toxicology

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“…All values are expressed as mean ± SE (n = 3) heavy metals on bacterial resistance to antibiotics worth detailed study. SOD is one of the important antioxidants, which overcome oxidative stress and maintain bacterial survival under stress, and are stimulated in turn by antibiotics and heavy metals in resistant bacterial strains (Liu et al 2012;Abskharon et al 2010). It is found that heavy metals (Cu, Zn, and Ag) can elevate not only TJ-Cu/Zn SOD and TJ-Mn-SOD mRNA levels but also total SOD activities .…”

Section: Effects Of Zn Concentration and Binary Exposure On Bacterialmentioning

confidence: 99%

Antioxidative responses of Pseudomonas fluorescens YZ2 to simultaneous exposure of Zn and Cefradine

Chen

et al. 2015

Ecotoxicology

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Binary pollution of both heavy metals and antibiotics has received increasing attentions for their joint effects of eco-toxicity and health hazards. To reveal the effects of mixtures of different pollutants on bacterial antioxidant response system, Pseudomonas fluorescens ZY2, a new strain isolated from swine wastewater, was chosen to determinate growth (bacterial density OD600), reactive oxygen species (ROS) concentration, protein concentration and superoxide dismutase (SOD) activity under exposure treatments of Zn, Cefradine or Zn + Cefradine. Bacterial densities of all the treatment groups increased significantly over the incubation time, but those containing pollutant addition were slightly lower than the control at different times of incubation. Both ROS concentration and SOD activity increased first and then decreased (p < 0.01) over time, which was opposite to the protein concentrations (p < 0.01), showing a much significant increase by Cefradine alone. With Zn concentration increasing from 40 to 160 mg/L, the intracellular SOD activity increased as a response to the improvement of ROS (p < 0.05), while the balance between ROS and SOD was broken down due to the disproportionate change of total SOD activity and ROS concentration, the bacterial densities therefore decreased for the weak resistance. With the combined treatment of Zn (200 mg/L) and Cefradine (1 mg/L), though the toxicity of Zn caused a much significant increase of ROS, the bacterial resistance was further improved showing a more significant increase of total SOD activity and the bacterial densities therefore increased bacterial growth. Zn concentration also affected the protein synthesis. Either single or binary stress induced the bacterial resistance by regulating SOD activity to eliminate ROS. All results of the bacterial oxidant stress, SOD response and protein synthesis in the combined treatment groups were more complicated than those in single treatment groups, which depended on the properties of the single treatment as well as the interaction between the two treatments upon bacterial activity. For P. fluorescens ZY2, the mediation of SOD activity to eliminate ROS in response to the combined exposure to Zn and Cefradine was first revealed as one of the co-resistance mechanisms, which is informative to further understanding the risk of antibiotics resistant bacteria to human and environmental health more accurately.

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Antioxidant responses and degradation of two antibiotic contaminants in Microcystis aeruginosa

Cited by 123 publications

References 63 publications

Hormesis Effects of Amoxicillin on Growth and Cellular Biosynthesis of Microcystis aeruginosa at Different Nitrogen Levels

Hormesis Effects of Amoxicillin on Growth and Cellular Biosynthesis of Microcystis aeruginosa at Different Nitrogen Levels

Interactions between the antimicrobial agent triclosan and the bloom-forming cyanobacteria Microcystis aeruginosa

Antioxidative responses of Pseudomonas fluorescens YZ2 to simultaneous exposure of Zn and Cefradine

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