Antioxidant Protective Effect of Glibenclamide and Metformin in Combination with Honey in Pancreas of Streptozotocin-Induced Diabetic Rats

Abstract

Hyperglycemia exerts toxic effects on the pancreatic β-cells. This study investigated the hypothesis that the common antidiabetic drugs glibenclamide and metformin, in combination with tualang honey, offer additional protection for the pancreas of streptozotocin (STZ)-induced diabetic rats against oxidative stress and damage. Diabetes was induced in male Sprague Dawley rats by a single dose of STZ (60 mg/kg; ip). Diabetic rats had significantly elevated levels of lipid peroxidation (TBARS), up-regulated activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) while catalase (CAT) activity was significantly reduced. Glibenclamide and metformin produced no significant effects on TBARS and antioxidant enzymes except GPx in diabetic rats. In contrast, the combination of glibenclamide, metformin and honey significantly up-regulated CAT activity and down-regulated GPx activity while TBARS levels were significantly reduced. These findings suggest that tualang honey potentiates the effect of glibenclamide and metformin to protect diabetic rat pancreas against oxidative stress and damage.

Neuroprotective effects of Tualang honey against oxidative stress and memory decline in young and aged rats exposed to noise stress

“…Other studies have also demonstrated that honey is able to increase antioxidant enzymes levels and ameliorate oxidative stress in plasma and other tissues such as renal and pancreas [61][62][63][64][65][66][67][68]. It is suggested that the aforementioned flavonoids contents and enzymatic and non-enzymatic substances in honey are responsible for its antioxidative effects.…”

Effect of Nenas honey supplementation on the oxidative status of undergraduate students

“…This might be the result of lower amount of free radicals available to activate the antioxidant enzymes as shown by reduction in oxidative DNA damage among the supplemented subjects. This also indicates that honey attenuates the changes in antioxidant enzymes in response to oxidant generation (EREJUWA et al, 2010).…”

“…Honey is one of the natural food products that is well-known for its high nutritional and prophylactic medicinal value (PYRZYNSKA & BIESAGA, 2009). The antioxidative properties in honey have been proven effective in aiding the healing process of wounds and burns, diabetic ulcers, cancer, cardiovascular and neural diseases (TAN et al, 2009;EREJUWA et al, 2010;KHALIL et al, 2010). In addition, honey also helps to improve spatial memory and reduces the anxiety in aging mice, and has antimicrobial properties that act effectively against human pathogenic microorganisms (PAUL et al, 2007;CHEPULIS et al, 2009).…”

“…Therefore, it is vital to cater the availability of antioxidants by manipulating diet and life style of individuals in aid of the existing network. In an effort to combat the free radical activities, various studies have been done to determine the effect of diet and dietary supplementation in increasing the individual's antioxidant level (GHELDOF & ENGESETH, 2002;RATNAM et al, 2006;EREJUWA et al, 2010). Antioxidant properties of honey have often been highlighted because they act as protective elements against aging and other age related diseases by scavenging ROS (KNOWLDEN, 2012).…”

Survival Time and Histological Outcome of Major Organs Following Honey Administration to Acute Paraquat-Intoxicated Rats: A Preliminary Study

“…Tualang honey (TH) is a wild honey harvested from Tualang trees found in many Asian rainforests. Various studies have been conducted in an effort to evaluate the possible medicinal uses of TH [18][19][20][21][22][23][24]. One of the proposed therapeutic values is attributed partly due to its antioxidant properties.…”