Antioxidant properties of some different molecular weight chitosans

Tomida, Hisao; Fujii, Takeshi; Furutani, Nobuko; Michihara, Akihiro; Yasufuku, Taira; Akasaki, Kenji; Maruyama, Toru; Otagiri, Masaki; Gebicki, Janusz M.; Anraku, Makoto

doi:10.1016/j.carres.2009.05.006

Cited by 138 publications

(58 citation statements)

References 42 publications

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“…Over the years, polysaccharides, such as CHI, were widely used as antioxidants to prevent oxidative damage of animals (Tomida et al 2009). …”

Section: Discussionmentioning

confidence: 99%

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Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattle

Li¹,

Na²,

Yu³

et al. 2015

CZECH J ANIM SCI

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The present experiment was conducted to study the effects of chitosan (CHI) on immune and antioxidative function in beef cattle. A total of 24 fattening Simmental cattle with similar body weight and age were divided randomly into three dietary groups, and the three diets contained 0, 500, and 1000 mg/kg CHI, respectively. The feeding trial lasted for 84 days. It was found that: (1) the addition of CHI in diets improved (P < 0.05) the levels of IgA and interleukin-1, and decreased (P < 0.1) the levels of soluble cluster of differentiation 4 receptor in serum at middle stage except that IgA remained unchanged in 1000 mg/kg CHI group. The levels of IgM and IgA tended to be increased (P < 0.1) by dietary CHI at later stage of the experiment; (2) the addition of 500 mg/kg CHI in diets increased (P < 0.1) total superoxide dismutase activity and decreased (P < 0.05) malondialdehyde content in serum at early and later stages, respectively. In conclusion, these results indicated that addition of 500 mg/kg CHI affected humoral and cellular immune responses, and improved the antioxidative function of beef cattle.

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“…Over the years, polysaccharides, such as CHI, were widely used as antioxidants to prevent oxidative damage of animals (Tomida et al 2009). …”

Section: Discussionmentioning

confidence: 99%

“…It showed the effects of scavenging free radicals and protecting the body against damage from peroxides (Tomida et al 2009). CHI connecting with some groups (Guo et al 2008;Sun et al 2008) or being absorbed into nanoparticles (Esumi et al 2003) exhibited a better antioxidative activity than a single component.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattle

Li¹,

Na²,

Yu³

et al. 2015

CZECH J ANIM SCI

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“…Although used in various diseases, it has been suspected that most curative effects of polysaccharides were based on their antioxidant activity. Certain type of antioxidative reaction in certain types of cells leads of treatment to specific disease (Li et al 2009;Tomida et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Crude polysaccharide from an anti-UVB cell clone of Bupleurum scorzonerifolium protect HaCaT cells against UVB-induced oxidative stress

et al. 2011

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Bupleurum scorzonerifolium Willd has been found to have a wide range of immunopharmacologic functions. We isolated an anti-UVB B. scorzonerifolium cell clone and found elevated level of polysaccharides. In this study, we investigated the ability of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone to inhibit UVBinduced photodamage using a human skin keratinocyte cell line, HaCaT. Cells were UVB irradiated and then incubated in presence of different concentrations of CP. MTT assay showed that the CP did not induce cytotoxic effect under 10 mg/mL and after UVB irradiation, CP can inhibit UVB-induced HaCaT cell death. Decreased reactive oxygen species and lipid peroxidation and increased superoxide dismutase activity showed that CP can act as a free radical scavenger. Furthermore, CP had a strong protective ability against UVB-induced DNA damage. These effects were compared to the crude polysaccharide (CP 0 ) from normal B. scorzonerifolium callus at concentration of 20 mg/mL. The portion of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone was more than 2.5-fold higher than crude polysaccharide (CP 0 ) from normal B. scorzonerifolium callus. Taken together, the protective mechanisms of crude polysaccharide from the anti-UVB B. scorzonerifolium cell clone against UVB-induced photodamage occur by the inhibition of UVB-induced reactive oxygen species production, lipid peroxidation and DNA damage.Keywords Anti-UVB Bupleurum scorzonerifolium cell clone Á Crude polysaccharide Á Oxidative stress Á Photodamage Á UVB

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“…Adicionalmente, a quitosana apresenta propriedades favoráveis de biocompatibilidade e bioatividade, além de ser atóxica, bactericida, fungicida e biodegradável e agir como composto cicatrizante, apresentando vantagem em relação aos polímeros sintéticos que, na sua grande maioria, são tóxicos [30][31][32] .…”

Section: Ensaio Biológicounclassified

Caracterização química e efeito cicatrizante de quitosana, com baixos valores de massa molar e grau de acetilação, em lesões cutâneas

et al. 2015

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ResumoInúmeras pesquisas buscam fármacos com propriedades cicatrizantes, capazes de reparar lesões cutâneas. O objetivo deste estudo foi caracterizar quimicamente uma quitosana e avaliar seus efeitos cicatrizantes em lesões cutâneas de ratos. A quitosana foi caracterizada quanto à viscosidade, massa molar, grau de acetilação, análise elementar, termogravimetria e espectroscopia na região do infravermelho. As propriedades cicatrizantes da quitosana foram avaliadas, para isso ratos adultos foram submetidos à excisão tecidual e tratados por 21 dias, sendo avaliados parâmetros macroscópicos, semanalmente, e microscópicos, ao final do experimento. A quitosana apresentou viscosidade de 5 mPa s, baixo grau de acetilação (GA=5,9%) e massa molar (132,0 kDa). Por análise elementar observou-se que a composição da quitosana se aproxima da fórmula C 6 H 11 O 4 N, suas principais bandas características foram observadas no espectro de infravermelho e a curva termogravimétrica mostrou que esta apresenta somente duas etapas de decomposição. Não houve diferença significativa na histologia, no 21º dia da lesão, entre os grupos controle e tratado, porém, macroscopicamente, a quitosana promoveu redução de 81,4% da largura das lesões, porcentagem significativamente superior à observada no grupo controle (71,2%), auxiliando a cura de lesões cutâneas, o que reforça seu potencial de aplicação médica. Palavras-chaves: grau de acetilação, análise termogravimétrica, propriedades cicatrizantes, quitosana. AbstractSeveral researches look for drugs with healing properties, capable to cure skin lesions. The objective of this study was to chemically characterize one chitosan and evaluate its healing effects in mice skin lesions. The chitosan was characterized in relation to the viscosity, molar mass, acetylation degree, elementary analysis, thermogravimetry and infrared spectroscopy. To evaluate the healing properties of chitosan mices were submitted to tissue excision and treated during 21 days. The macroscopic parameters were weekly evaluated and the microscopic parameters at the end of the experiment. Chitosan presented viscosity of 5 mPa s, low acetylation degree (GA= 5.9%) and molar mass of 132.0 kDa. By elemental analysis, we observed that the composition of chitosan is similar to the formula C 6 H 11 O 4 N, its main characteristic bands were observed in the infrared spectrum and the thermogravimetric curve showed two decomposition steps. There was no significant difference on the histology between the control and the treated group after the 21 st day of lesion. Macroscopically, chitosan caused the reduction of 81.4% of the lesions width, which is significantly superior to the observed reduction in the control group (71.2%), thus helping the cure of skin lesions. These results reinforces its potential use for medical application.Keywords: degree of acetylation, thermogravimetric analysis, healing properties, chitosan. IntroduçãoA pele reveste quase a totalidade da superfície corpórea e quando um dano tecidual compromete a continuidade des...

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Antioxidant properties of some different molecular weight chitosans

Cited by 138 publications

References 42 publications

Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattle

Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattle

Crude polysaccharide from an anti-UVB cell clone of Bupleurum scorzonerifolium protect HaCaT cells against UVB-induced oxidative stress

Caracterização química e efeito cicatrizante de quitosana, com baixos valores de massa molar e grau de acetilação, em lesões cutâneas

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