Antioxidant Properties of Protein Hydrolysate Obtained from OysterSaccostrea cucullata(Born, 1778)

Abstract

The antioxidant activities of enzymatically hydrolyzed (protease from Bacillus cereus SU12) oyster (Saccostrea cucullata) protein were studied. The hydrolysate exhibited a strong antioxidant potential in 1, 1-diphenyl-2-picrylhydrazyl (DPPH, 85.7 ± 0.37%), followed by hydrogen peroxide radical scavenging activity (81.6 ± 0.3%), hydroxyl radical scavenging activity (79.32 ± 0.6%), and reducing power assay (2.63 ± 0.2 OD at 700 nm) at a concentration of 1 mg/mL. Due to the high antioxidant potential, the hydrolysate was purified in Sephadex G-25 gel filtration chromatography. The active peptide fraction was identified by DPPH and reducing power assay. The amino acid content of the purified active peptide fraction was analyzed by high performance liquid chromatography. The active fraction contained a good quantity of both essential and nonessential amino acids. The present study revealed that oyster (S. cucullata) protein hydrolysate is a potential source for natural antioxidants.

Antiradical Activity of Hydrolysates and Extracts from Mollusk A. broughtonii and Practical Application to the Stabilization of Lipids

“…Similarly to our result, the protein hydrolysate obtained from the oyster Saccostrea cucullata exhibited 85.7% [10], the hydrolysate of the common smooth hound (Mustelus mustelus) exhibited 76.7% of scavenging activity [36], and protein hydrolysate of Conus betulinus exhibited 84% of scavenging activity [37]. By comparing the percentage of radical scavenging activity, the acid and enzymatic hydrolysates have proven to be a potential source for antiradical activity.…”

“…At the same time, raw materials of animal-including marine-origin, and products (of animal/marine origin) that have been subjected to technological and biotechnological modification could also have antioxidants. The biological and pharmacological activities of organic natural compounds of marine origin have been demonstrated by numerous studies [10][11][12][13].…”

Physicochemical properties of hydrophobic and hydrophilic peptides from oyster protein

“…Umayaparvathi et al . (2014) found that oyster protein hydrolysates have potent antioxidant properties; the results are similar to Dong et al . (2010).…”

“…Possible bioactivities of oyster peptides have been reported. Umayaparvathi et al (2014) found that oyster protein hydrolysates have potent antioxidant properties; the results are similar to Dong et al (2010). Cheng et al (2018) discovered a new anticoagulant peptide that could potently prolong the activated partial thromboplastin time and the thrombin time, and this peptide was purified and identified from the pepsin hydrolysate of oyster.…”

Isolation and characterization of antioxidant peptides from oyster (Crassostrea rivularis) protein enzymatic hydrolysates

“…Moreover, F5 possessed high DPPH, hydroxyl, and superoxide radical-scavenging activities of 90.16%, 81.15 U/ml, and 108.20 U/g at 1 mg/ml, respectively, with similar activities not achieved by the other peptides. In contrast, the DPPH radical-scavenging activity of the peptide from S. cucullata at 1 mg/ml was only 85.7% (Umayaparvathi et al, 2015).…”