Antioxidant Properties of Mushroom Mycelia Obtained by Batch Cultivation and Tocopherol Content Affected by Extraction Procedures

Abstract

The determination of the antioxidant potential of lyophilized mushroom mycelia from 5 strains of the species Pleurotus ostreatus and Coprinus comatus (obtained by submerged cultivation in batch system) was analyzed as ethanolic extracts by evaluating ABTS and the hydroxyl scavenging activity, FRAP method, the chelating capacity, the inhibition of human erythrocyte hemolysis, and the inhibition of xanthine oxidase activity. The main compounds present in all extracts were determined by HPLC chromatography. Overall, results demonstrated that the biologically active substances content is modulated by the extraction method used. The most beneficial extract, characterized by determining the EC50 value, was that of C. comatus M8102, followed by P. ostreatus PQMZ91109. Significant amount of α-tocopherol (179.51 ± 1.51 mg/100 g extract) was determined as well as flavones such as rutin and apigenin. In the P. ostreatus PQMZ91109 extract, 4.8 ± 0.05 mg/100 g extract of tocopherol acetate known to play a significant role as an antioxidant in skin protection against oxidative stress generated by UV rays was determined. The various correlations (r 2 = 0.7665–0.9426 for tocopherol content) assessed and the composition of extracts in fluidized bed from the mycelia of the tested species depicted a significant pharmacological potential as well as the possibility of usage in the development of new functional products.

The effects of different solvents on bioactive metabolites and “in vitro” antioxidant and anti-acetylcholinesterase activity of Ganoderma lucidum fruiting body and primordia extracts

“…De Bruin et al investigated the antioxidant properties of extracts obtained from Grifola gargal mushrooms, which are of the same order as our results [39].…”

Fibre degradation of wheat straw by Pleurotus erygnii under low moisture conditions during solid-state fermentation

“…Wood-rot fungi that have a high tolerance to low moisture conditions with the ability to grow at much higher solid to liquid ratios could have useful applications in solid-state fermentation of agricultural waste products (Wan and Li 2012). Some of the benefits would be lower energy requirements to regulate the temperature of the fermentation, which could significantly increase with larger quantities of waste and the opportunity to extract desirable products using organic solvents (Vamanu 2014). Most studies using agricultural waste substrates reveal that higher moisture contents support optimal growth of wood-rot fungi (Asgher et al 2006;Shi et al 2008), leading to significantly higher levels of lignin degradation (Shi et al 2008).…”

Antioxidative Potential, Antibiofilm Activity and Partial Chemical Characterization of Cultured Broth after Pleurotus ferulae Mycelium Cultivation

“…The influence of polysaccharide fraction has been directly dependent on the structure of the polymer, which may withhold even low quantities of phenolic compounds [3]. Although the purpose of the present study has not been represented by the analysis of the biological effect stated by these very compounds, one has noticed a certain direct relationship between the quantity being present in the relevant medium and one of the relevant phenolic compounds.…”

“…The controlled growth of the mycelium of the duly concerned species can serve as an efficient alternative to getting bioactive compounds of pharmacological interest [3]. Some of the main bioactive compounds are the polysaccharides.…”