Antioxidant properties of Krebs cycle intermediates against malonate pro-oxidant activity in vitro: A comparative study using the colorimetric method and HPLC analysis to determine malondialdehyde in rat brain homogenates

Puntel, Robson Luiz; Roos, Daniel Henrique; Grotto, Denise; Garcia, Solange Cristina; Nogueira, Cristina W.; Rocha, João Batista Teixeira da

doi:10.1016/j.lfs.2007.04.023

Cited by 84 publications

(46 citation statements)

References 58 publications

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“…To the reaction mixture, 8.5 % sodium dodecyl sulfate (SDS), acetic acid/HCl (pH 3.4) and 0.6 % thiobarbituric acid (TBA) were subsequently added and the mixture was incubated at 100°C for 1 h. Lipid peroxidation (LPO) was measured by TBARS formation as described by Puntel et al (6). Color was read at 532 nm using an ELISA plate reader.…”

Section: In Vitro Fe 2+ -Induced Lipid Peroxidation In the Brainmentioning

confidence: 99%

In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

Kamdem¹,

Stefanello²,

Boligon³

et al. 2012

Acta Pharmaceutica

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Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC 50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL -1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g -1 of dry extract. Fe 2+ -induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca 2+ -induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Dy m only at high concentrations (40-100 mg mL -1 ), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.

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Section: In Vitro Fe 2+ -Induced Lipid Peroxidation In the Brainmentioning

confidence: 99%

In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

Kamdem¹,

Stefanello²,

Boligon³

et al. 2012

Acta Pharmaceutica

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“…Tissues were immediately homogenized in cold 10 mM Tris-HCl, pH 7.5 (1/10, w/v). The homogenate was centrifuged for 10 min at 4,000 9 g to yield a pellet that was discarded and a low-speed supernatant (S1) that was used for the TBARS assay [39].…”

Section: Tissue Preparationmentioning

confidence: 99%

Antioxidant Effects of Different Extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus

et al. 2008

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Considering the important role of oxidative stress in the pathogenesis of several neurological diseases, and the growing evidence of the presence of compounds with antioxidant properties in the plant extracts, the aim of the present study was to investigate the antioxidant capacity of three plants used in Brazil to treat neurological disorders: Melissa officinalis, Matricaria recutita and Cymbopogon citratus. The antioxidant effect of phenolic compounds commonly found in plant extracts, namely, quercetin, gallic acid, quercitrin and rutin was also examined for comparative purposes. Cerebral lipid peroxidation (assessed by TBARS) was induced by iron sulfate (10 microM), sodium nitroprusside (5 microM) or 3-nitropropionic acid (2 mM). Free radical scavenger properties and the chemical composition of plant extracts were assessed by 1'-1' Diphenyl-2' picrylhydrazyl (DPPH) method and by Thin Layer Chromatography (TLC), respectively. M. officinalis aqueous extract caused the highest decrease in TBARS production induced by all tested pro-oxidants. In the DPPH assay, M. officinalis presented also the best antioxidant effect, but, in this case, the antioxidant potencies were similar for the aqueous, methanolic and ethanolic extracts. Among the purified compounds, quercetin had the highest antioxidant activity followed by gallic acid, quercitrin and rutin. In this work, we have demonstrated that the plant extracts could protect against oxidative damage induced by various pro-oxidant agents that induce lipid peroxidation by different process. Thus, plant extracts could inhibit the generation of early chemical reactive species that subsequently initiate lipid peroxidation or, alternatively, they could block a common final pathway in the process of polyunsaturated fatty acids peroxidation. Our study indicates that M. officinalis could be considered an effective agent in the prevention of various neurological diseases associated with oxidative stress.

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“…Several studies have focused in the use of natural therapeutic antioxidant compounds that can afford protection in a variety of in vitro and in vivo models of human pathologies, including neurotoxicity models. 17 Boligon et al 14 and Zadra et al 20 working with medicinal plants, such as, S. buxifolia, Nasturtium officinale and Solanum guaraniticum found effects on lipid peroxidation in vitro, these species showed the great antioxidant activity that is directly related to the presence of phenolics compounds and flavonoids.…”

Section: Resultsmentioning

confidence: 99%

“…The concentration ranges of each tested extract were 0.1, 5, 10, 25, 50, 100 and 200 mg/mL. TBARS production was determined as described by Puntel et al 17 …”

Section: In Vitro Fe(ii)-induced Lipid Peroxidation In Rat Brainsmentioning

confidence: 99%

Phytochemical constituents and in vitro antioxidant capacity of Tabernaemontana catharinensis A. DC

Boligon

Freitas

Brum

et al. 2013

Free Radicals and Antioxidants

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a b s t r a c tIntroduction: Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. In this present study we determined the in vitro antioxidant capacity and quantified of total phenolics, flavonoids, tannins and alkaloids of Tabernaemontana catharinensis crude extract and fractions leaves. Methods: The antioxidant potential was evaluated by 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging and thiobarbituric acid reactive species (TBARS) methods, total phenolics content was determined using the FolineCiocalteu assay, flavonoids, tannins and alkaloids were determined by spectrophotometer. Results: Crude extract and fractions showed inhibition against TBARS, ethyl acetate was the most effective fraction (IC 50 ¼ 6.71 AE 0.19 mg/mL), subsequently by butanolic (26.15 AE 0.08 mg/mL), dichloromethane (43.25 AE 0.12 mg/mL) and crude extract (61.09 AE 0.05 mg/mL), respectively. Moreover, the DPPH assay, presented IC 50 value ranged of 4.64 AE 1.25 to 27.78 AE 0.93 mg/mL. Contents of total phenols, flavonoids, tannins and alkaloids of T. catharinensis followed the order: ethyl acetate > butanolic > dichloromethane fractions > crude extract. Conclusion: The present study, we found that the crude extract and fractions of T. catharinensis showed good antioxidant activity. Among the samples tested, the ethyl acetate fraction showed better activity than others.

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Antioxidant properties of Krebs cycle intermediates against malonate pro-oxidant activity in vitro: A comparative study using the colorimetric method and HPLC analysis to determine malondialdehyde in rat brain homogenates

Cited by 84 publications

References 58 publications

In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

Antioxidant Effects of Different Extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus

Phytochemical constituents and in vitro antioxidant capacity of Tabernaemontana catharinensis A. DC

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