Antioxidant properties of hispidulin

Kut, Kacper; Bartosz, Grzegorz; Soszyński, Mirosław; Sadowska-Bartosz, Izabela

doi:10.1080/14786419.2022.2032050

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…Among the specialized metabolites identified in our study, particularly 1,5-dicaffeoylquinic acid has been related to a strong antioxidant activity [ 28 ]. Moreover, out of the flavonols detected, luteolin has been previously reported for its antioxidant capacity [ 29 ], while hispidulin showed contrasting results, being not active in cell-free systems but effective in the protection against erythrocyte membrane lipid peroxidation [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

A Nanotechnological Approach to Exploit and Enhance the Bioactivity of an Extract from Onopordum illyricum L. Leaves

Caddeo

Tuberoso

Floris

et al. 2023

Plants

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Plant-derived products have been used for preventive and curative purposes from the ancient era to the present day. Several studies have demonstrated the efficacy of either multicomponent-based extracts, enriched fractions, or isolated bioactives. However, they often display low solubility and bioavailability, chemical instability, poor absorption, and even toxicity, which restrict application in therapy. The use of drug delivery systems, especially nanocarriers, can overcome these physicochemical and pharmacokinetic limitations. In this study, an extract from Onopordum illyricum leaves was produced by maceration in 80% ethanol, characterized by liquid chromatography coupled to mass spectrometry, and formulated in phospholipid vesicles with the aim of exploiting and possibly enhancing its bioactivity for skin delivery. The results showed that phenolic compounds were abundantly present in the extract, especially hydroxycinnamic acid and flavonol derivatives. The extract-loaded vesicles showed small size (<100 nm), high entrapment efficiency (even >90% for most phenolic compounds), and good long-term stability. Moreover, the extract-loaded vesicles exhibited remarkable antioxidant activity, as demonstrated by colorimetric assays and by enhanced reduction of intracellular reactive oxygen species (ROS) levels in cultured skin cells. Hence, our findings support the key role of nanotechnological approaches to promote the potential of plant extracts and strengthen their application in therapy.

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Section: Discussionmentioning

confidence: 99%

A Nanotechnological Approach to Exploit and Enhance the Bioactivity of an Extract from Onopordum illyricum L. Leaves

Caddeo

Tuberoso

Floris

et al. 2023

Plants

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“…Numerous studies have shown that LPS-injected animals display marked oxidative stress and tubular cell apoptosis in the kidneys [ 46 ]. Hispidulin has been known to possess an antioxidant property [ 48 ]. In this study, LPS injection induced an increase in lipid and DNA oxidation products.…”

Section: Discussionmentioning

confidence: 99%

Hispidulin Ameliorates Endotoxin-Induced Acute Kidney Injury in Mice

Kim

Leem

2022

Molecules

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Lipopolysaccharide (LPS) is an endotoxin that plays a crucial role in septic acute kidney injury (AKI). Hispidulin is a natural flavonoid that possesses various biological activities. Recent studies have shown that hispidulin administration alleviates various inflammatory diseases in animal models. This study aimed to investigate the renoprotective effect of hispidulin on LPS-induced AKI. Male C57BL/6 mice were administered LPS (10 mg/kg) with or without hispidulin (50 mg/kg). Hispidulin administration attenuated renal dysfunction, histological alterations, and the upregulation of neutrophil gelatinase-associated lipocalin. This flavonoid also reduced cytokine production and Toll-like receptor 4 expression, inhibited nuclear factor-κB and mitogen-activated protein kinase cascades, and alleviated immune cell infiltration. The oxidation of lipids and DNA was also inhibited by hispidulin administration. This antioxidant effect of hispidulin was associated with the downregulation of NADPH oxidase 4, the activation of catalase and superoxide dismutase activities, and the restoration of glutathione levels. Moreover, hispidulin administration attenuated tubular cell apoptosis by inhibiting caspase-3 pathway. These data suggest that hispidulin ameliorates endotoxin-induced kidney injury by suppressing inflammation, oxidative stress, and tubular cell death.

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“…ABTS • reacts with many substances, its reactivity being higher than that of DPPH and Fe 3+ in the FRAP assay. For example, we found that the reactivity of hispidulin with ABTS • was 2.82 mol TE/mol hispidulin, while its reactivity with DPPH and Fe 3+ (FRAP assay) was negligible (0.019 and 0.09 mol TE/mol hispidulin, respectively) [43]. Similarly, capsaicin showed a reactivity with ABTS • of 0.74 (Table 1), a reactivity in the FRAP assay of 1.103 mol TE/mol, and only 0.079 mol TE/mol with DPPH (submitted).…”

Section: Alternative Parameters To Measure Antioxidant Activitymentioning

confidence: 97%

A Modification of the ABTS• Decolorization Method and an Insight into Its Mechanism

et al. 2022

Self Cite

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A modification of the ABTS• decolorization assay for plate readers is presented. In our modification, 200 µL of ABTS solution of absorbance 1.0 at 734 nm was added with an antioxidant and decreased absorbance resulted. For comparison of antioxidant activities in the kinetic assay of absorbance decrease, concentration dependence of absorbance decrease and of area under curve are recommended. “Fast” and “slow” antioxidants were distinguished: while the reactions of “fast” antioxidants ABTS• were completed within seconds, the reactions of “slow” antioxidants were not finished after 6 min. We recommend reaction time of 60 min for assays of such antioxidants, blood plasma and plant extracts. Sub-additive interactions between some antioxidants (ascorbate and Trolox, hispidulin and Trolox, and glutathione and ascorbate) were found in the ABTS• decolorization; possible reasons for such interactions are discussed.

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Antioxidant properties of hispidulin

Cited by 8 publications

References 15 publications

A Nanotechnological Approach to Exploit and Enhance the Bioactivity of an Extract from Onopordum illyricum L. Leaves

A Nanotechnological Approach to Exploit and Enhance the Bioactivity of an Extract from Onopordum illyricum L. Leaves

Hispidulin Ameliorates Endotoxin-Induced Acute Kidney Injury in Mice

A Modification of the ABTS• Decolorization Method and an Insight into Its Mechanism

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