Antioxidant properties of albumin: effect on oxidative metabolism of human neutrophil granulocytes

Kouoh, Ferdinand; Gressier, Bernard; Luyckx, M.; Brunet, C.; Dine, T.; Cazin, M.; Cazin, J. C.

doi:10.1016/s0014-827x(99)00082-8

Cited by 65 publications

(50 citation statements)

References 20 publications

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“…The serum ALT concentrations have been reported to increase in monkeys fed a high protein diet 36 , and the increased ALT level with a very small magnitude in the present study is considered to be a similar phenomenon. Human serum ALB has been reported to generally inhibit the neutrophil respiratory burst 37 and its adhesion to vascular cells 31 . In another report 38 , however, some commercially available human ALB solutions increased expression of the adhesion molecules in cultured human umbilical venous endothelial cells, but others did not.…”

Section: Discussionmentioning

confidence: 99%

Aggravation of Galactosamine Hepatotoxicity by Albumin in Rats

et al. 2008

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Abstract:The effect of rat albumin (RALB) on D-galactosamine (GalN) hepatotoxicity was investigated in male Crl:CD(SD) rats aged 6 weeks. The animals were divided into control, RALB, GalN, and RALB + GalN groups. GalN (800 mg/kg) was intraperitoneally administered immediately after an intravenous injection of RALB (100 mg/kg). The animals were euthanized 6 or 24 h later and subjected to laboratory investigations and histological examinations of the liver. Furthermore, in order to determine the contributing factors of the effect on the hepatotoxicity, intra-and intergroup comparisons were made between liver-injured and normal animals for parameters showing marked fluctuations at 6 h post-dosing. As a result, RALB induced fluctuations in many parameters, but no histological changes were observed in the liver at both 6 and 24 h. GalN induced mild or moderate hepatocyte necrosis (necrosis and/or apoptosis of hepatocytes) at 6 and 24 h, respectively, accompanied by increases in the serum ALT and AST levels. Concurrent administration of RALB with GalN markedly aggravated the GalN-induced hepatotoxicity, as shown by severe hepatocyte necrosis accompanied by further increases in the serum ALT, AST, and T-BIL levels in the surviving animals at 6 and 24 h, and by very severe hepatocyte necrosis with congestion in the dead animals. Moreover, serum tumor necrosis factor-α (TNF-α) level was also increased at 6 h in the RALB + GalN group, but not in the RALB or GalN alone groups. In conclusion, RALB was shown to aggravate GalN hepatotoxicity in rats, and the increased serum TNF-α level is considered to be a contributing factor of the aggravation. (J Toxicol Pathol 2008; 21: 175-183)

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Section: Discussionmentioning

confidence: 99%

Aggravation of Galactosamine Hepatotoxicity by Albumin in Rats

et al. 2008

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“…Many food proteins, including milk proteins, such as lactoferrin, b-lactoglobulin and casein, soy proteins, mushroom proteins, egg albumen proteins and egg yolk phosvitin, maize zein, potato patatin and yam dioscorin, were reported to have antioxidant activity (Chiue, Kusano, & Iwami, 1997;Hou et al, 2001;Kouoh et al, 1999;Liu, Han, Lee, Hsu, & Hou, 2003;Maheswari, Ramadoss, & Krishnaswamy, 1997;Rajalakshmi & Narasimhan, 1996;Satue-Gracia, Frankel, Rangavajhyala, & German, 2000;Zhao et al, 2004). The proteins owe their antioxidant activity to their constituent amino acids.…”

Section: Introductionmentioning

confidence: 99%

“…For example, the antioxidant activities of aromatic amino acids such as In plants and animals, the major water-soluble protein fraction is formed by albumins (Damodaran, 1996). In the literature, the antioxidant activities of albumins from legumes, such as broad beans, white and brown beans and light peas (Okada & Okada, 1998;Wolosiak & Klepacka, 2002) and bovine and human serum (Aime et al, 2003;Fukuzawa et al, 2005;Kouoh et al, 1999;Wolosiak & Klepacka, 2002), have been well documented. The emulsifying and foaming activity of animal and plant origin albumins has also been reported by different workers (AlMalah et al, 2000;Burnett et al, 2002;Damodaran, 1996;Ma & Harwalkar, 1984;Pearce & Kinsella, 1978;Tong, Sasaki, McClements, & Decker, 2000).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of protein extracts from heat-treated or thermally processed chickpeas and white beans

Arcan

Yemenicioğlu

2007

Food Chemistry

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In this study, antioxidant activities of water-soluble protein extracts from chickpeas and white beans were investigated. The area under the curve (AUC) values of lyophilized crude protein extracts (dialyzed or undialyzed) from thermally processed (121°C for 20 min) or heat-treated (90°C for 20 min) chickpeas (73-91 lmol trolox/g) and white beans (39-67 lmol trolox/g) indicated a higher free radical-scavenging capacity and thermostability for chickpea proteins than for white bean proteins. The thermal processing also increased the Fe +2 -chelating capacity of lyophilized chickpea crude protein extracts 1.8-fold whereas it caused a 2.3-fold reduction in the Fe +2 -chelating capacity of lyophilized white bean crude protein extracts. Dialysis increased the protein content of lyophilized chickpea extracts 1.5-2-fold but it did not affect the protein content of lyophilized white bean extracts significantly. Ammonium sulfate precipitation was not effective for selective precipitation of antioxidant proteins. However, it improved the free radical-scavenging capacity of lyophilized protein extracts from thermally processed chickpeas and white beans by almost 25% and 100%, respectively. DEAE-cellulose chromatography, indicated the presence of five (A 1 -A 5 ) and three (B 1 -B 3 ) antioxidant protein fractions in heat-treated and thermally processed chickpea protein extracts, respectively, and can be used for the partial purification of antioxidant proteins. The results of this study showed the good potential of chickpea proteins as thermostable natural food antioxidants.

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“…Concentrations of albumin exceeding in-vivo physiological concentrations may cause proteinaceous interference with observed toxicity 149 as well as reduce particle uptake 150 . Albumin also exhibits antioxidant properties which have the potential to diminish redox endpoints 151 , although the oxidative interactions of transition metals has been reported as similar under either BALF or synthetic bronchioalveolar fluid 143 . DMSO finds occasional use as a non-biological dispersant but has inferior performance and interference with oxidative processes is again an issue 147 .…”

Section: Bronchioalveolar Fluid Interactions With Pmmentioning

confidence: 99%

“…While albumin concentrations as high as 15 mg/mL are shown to be required to stabilise some nanoparticle suspensions 145 , the resulting increased specific surface area of the suspension may be counteracted by reduced catalytic activity in the been shown using BSA that albumin carries antioxidant effects, which may interfere with the oxidative stress mechanism of cellular damage 151 , and further reduce intracellular nanoparticle uptake 150 .…”

Section: Lung Surfactant and Size-dependent Effectsmentioning

confidence: 99%

In-vitro toxicology of respirable iron-rich particles relevant to mining industry: a high throughput framework for interpreting environmental particulate matter toxicity

Prakash¹

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This project investigated the cytotoxicity profiles and toxicological properties of inhalable iron-rich particles generated by mining activity. National Environment Protection Measure (NEPM) ambient air quality regulations specifically regulate sub-10 µm (PM10) and sub-2.5 µm (PM2.5) diameter particle fractions corresponding to thoracic and respirable lung penetration, respectively. However, atmospheric concentrations of fugitive iron-rich particulates in iron ore mining and processing associated settlements raised concerns that NEPM limits may inadequately address the specific toxicological profile of iron-rich PM10 and potentially adsorbed organics in these regions. To address this, comparative cytotoxicity experiments were carried out in-vitro using a variety of particulate standards, featuring other frequently studied metal-oxides, to help bridge in-vivo and epidemiological knowledge in the literature with in-vitro toxicological results. This also required the development of a novel framework to interpret physical and physiological characteristics of coarse, poorly soluble metal-oxide particles by conducting a critical systematic literature review.Analysis by the ChemCentre, WA was taken into account with relation to potentially absorbed organics and the influence of Polycyclic Aromatic Hydrocarbons (PAHs) investigated.The protocols developed by this project are applicable to high-throughput assessment of particulate matter generated by mining activity, having defined methodology to rapidly acquire sufficient quantities of PM10 for assays which can be multiplexed and the effects of particulate interference on assays minimised. This includes the integration of non-destructive sterilisation techniques, physical characterising by particle-sizing, particle solubility analysis and observation of particle morphology by electron microscopy and elemental analysis. Epithelial (A549), fibroblast (LL24) and monocyte differentiated macrophage (U937) cell lines were tested to build a picture of particulate effects on the major lung cell types in the lung parenchyma; the alveolar epithelium, connective tissue and immune system.The effect of lung fluid surfactants on cytotoxicity was determined, and advanced tissue culture methods were trialled to improve the differentiation between particles provided by the endpoints.ii The ore samples from the Mining Area C (MAC), Yandi and Newman Hub mines in the Pilbara region were found to have an effect comparable to the cytotoxicity of micron-size iron oxide standards and selected standards of titanium dioxide, silicon dioxide and carbon black, whereas copper oxide was found to have some ten-fold greater cytotoxicity across the cell lines. However cytotoxicity as a principle differentiator of toxicity was found to be insufficient in differentiating the physiological response to iron-rich PM10 and insoluble metal-oxide standards. At high concentrations the dose-response relationship plateaued at a maximum value for iron-oxide standards and iron ore samples in the A549 and LL2...

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Antioxidant properties of albumin: effect on oxidative metabolism of human neutrophil granulocytes

Cited by 65 publications

References 20 publications

Aggravation of Galactosamine Hepatotoxicity by Albumin in Rats

Aggravation of Galactosamine Hepatotoxicity by Albumin in Rats

Antioxidant activity of protein extracts from heat-treated or thermally processed chickpeas and white beans

In-vitro toxicology of respirable iron-rich particles relevant to mining industry: a high throughput framework for interpreting environmental particulate matter toxicity

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