Antioxidant properties of 3-deoxyanthocyanidins and polyphenolic extracts from Côte d’Ivoire’s red and white sorghums assessed by ORAC and in vitro LDL oxidisability tests

Abstract

Red sorghum is a source of phenolic compounds (PCs), including 3-deoxyanthocyanidins that may protect against oxidative stress related disease such as atherosclerosis. HPLC was used to characterise and quantify PCs extracted from red or white sorghum whole grain flour. Antioxidant activity was measured by an oxygen radical absorbance capacity assay and against LDL-oxidisability, and further compared to that of synthesised 3-deoxyanthocyanidins (i.e., luteolinidin and apigeninidin). Phenolic content of red and white sorghums was evaluated as 3.90 ± 0.01 and 0.07 ± 0.01 mmol gallic acid equivalents L(-1), respectively. Luteolinidin and apigeninidin were mainly found in red sorghum. Red sorghum had almost 3 and 10 times greater specific antioxidant activity compared to luteolinidin and apigeninidin, respectively. Red sorghum PCs and the two 3-deoxyanthocyanidins were also effective at preventing LDL vitamin E depletion and conjugated diene production. Red sorghum flour exhibits antioxidant capacity suggesting that it may be a valuable health-promoting food.

Phenolic Compounds and Antioxidant Capacities of 10 Common Edible Flowers from China

“…The ORAC procedure was performed using a Fluoroskan Ascent FL plate reader (Thermo scientific, Franklin, Mass., U.S.A.) with 96‐well plates (Carbonneau and others ). Analyses were conducted in phosphate buffer (pH 7.4) at room temperature.…”

Sorghum: An Underutilized Cereal Whole Grain with the Potential to Assist in the Prevention of Chronic Disease

“…(101) A recent in vitro analysis of red sorghum flour extracts showed strong free radical scavenging activity as measured by an oxygen radical absorbance capacity (ORAC) assay and protection against LDL-oxidation, contributing to the evidence base for the potential of red sorghum as a valuable health-promoting food grain. (102) However, understanding the bioavailability of sorghum anthocyanins for the putative health-promoting effects in humans is a much-needed focus of future research.…”

Osmanthus fragrans Flower Extract and Acteoside Protect Against d-Galactose-Induced Aging in an ICR Mouse Model

“…One of the main PeGs is acteoside. Acteoside exhibits extensive biological activities, including antioxidant, 8 anti-inflammatory, 9 and hepatoprotective activities. 10 Acteoside from Chinese tea Ligustrum purpurascens kudingcha is an effective lipase inhibitor.…”