Antioxidant Properties and Transepithelial Transportation of Di‐/tripeptides Derived from Simulated Gastrointestinal Digestion of Pig Blood Cells Hydrolysates

Ni, Xiaojun; Zhai, Zhe; Xin, Zhongquan; Tan, Chunqing; Chen, Yiliang; Deng, Jinping; Liao, Pinfeng; Zhang, Limeng; Xiao, Zaili; Deng, Baichuan

doi:10.2991/efood.k.200422.001

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Resistance of cell was monitored by a resistor (MERS00002, MilliporeSigma, USA) in everyday culture to make sure the monolayer of cell lines. Resistance was controlled transepithelial electrical resistance (TEER) value between 300 and 1000 Ω•cm 2 , until it exceeds the extreme growth [22]. Extremum of Caco-2 ALP was calculated by the ALP activity ratio of AL/BP of each well.…”

Section: Transwell Assaymentioning

confidence: 99%

Oral Administration of Oyster Peptide Prevents Bone Loss in Ovariectomized Mice

Chen

Shi

et al. 2020

eFood

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Osteoporosis is a disordered metabolic disease occurred congenitally or secondary. Although peptide agents have been approved to prevent osteoporosis administrated through subcutaneous injection, rare reports focused on peptide oral administration for preventive purpose. An eight peptide, P-CG-01 (YRGDVVPK) derived from oyster protein hydrolysates in gastrointestinal tract can promote the proliferation of osteoblast in vitro. In this study, ovariectomized mouse model was used to investigate the osteogenesis activity of P-CG-01. Bone mineral density value was recovered to the level of Sham by treatment of peptide [30 mg/kg body weight (BW)] in the ovariectomized mice. Peptide was detected at an average concentration of 803.16 ng/mL in serum from those mice after gavage. The peptide was labelled with fluorescein isothiocyanate and detected in the cytoplasm of osteoblast by confocal microscopes. ERK2 and BMP-2 expression was increased 1.5-and 3.8-fold, respectively, by the treatment of peptide. Our results suggest that P-CG-01 can inhibit the development of osteoporosis in ovariectomized mice.

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Section: Transwell Assaymentioning

confidence: 99%

Oral Administration of Oyster Peptide Prevents Bone Loss in Ovariectomized Mice

Chen

Shi

et al. 2020

eFood

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“…Generally, the amino acid length of food‐derived antioxidant peptides is 2–15 (Indiano‐Romacho et al., 2019) or 4–16 (Zaky et al., 2022). Notably, di‐/tripeptides can cross the intestinal epithelial barrier in their intact form to have biological effects (Ni et al., 2020). The antioxidant property of peptides is partially attributed to specific amino acids, such as leucine, isoleucine, proline (Y. Jiang et al., 2018), as well as tyrosine (C. Sun et al., 2019).…”

Section: Plant‐derived Bioactive Ingredients and Ckdmentioning

confidence: 99%

The modulation effects of plant‐derived bioactive ingredients on chronic kidney disease: Focus on the gut–kidney axis

et al. 2023

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Chronic kidney disease (CKD) results mainly from diabetes, hypertension, and glomerulonephritis, which can develop into end-stage renal disease and CKD-related complications, thereby causing high morbidity and mortality. This progression is correlated with pathogenic alterations in the gut microbiota that generate uremic toxins, mainly including indoxyl sulfate, p-cresol sulfate, and trimethylamine-N-oxide. The uremic toxins directly or indirectly induce CKD through oxidative stress, inflammatory response, and subsequently renal fibrosis. However, ongoing developments in CKD therapy still lack nutritional guidelines and new pharmacotherapies with significant effects. In this review, we discuss the modulatory effects of plant-derived bioactive ingredients, such as dietary fiber (oligosaccharides and polysaccharides), polyphenols (e.g., curcumin, anthocyanins, catechins, and resveratrol), and peptides, on gut microbiota and CKD particularly from the view of the gut-kidney axis. We believe that a comprehensive understanding of the underlying mechanisms of CKD is necessary to develop new targeted therapy strategies that will benefit the prevention and treatment of CKD.

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“…These peptides are usually characterized by low molecular weight (Mw), high activity, high safety, and easy absorption (Sarmadi & Ismail, 2010). Several studies have successfully separated antioxidant peptides from diverse food sources, including corn protein hydrolysates (Jin et al., 2016), corn silk protein (Chai et al., 2020), watermelon seeds protein (Wen et al., 2019), lotus seeds protein (Yu et al., 2021), and hazelnut protein (Shi et al., 2022), and even from by‐products, like pig blood cells (Ni et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Identification of potential antioxidant peptides from protein hydrolysates of pearl oil apricot almonds: Combination of in vitro and molecular docking studies

Chen

Guo

et al. 2023

Food Frontiers

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Pearl oil apricot almonds are rich in proteins, being a valuable raw material for preparing antioxidant peptides. In this study, almond protein hydrolysates (APHs) were obtained by enzymatic hydrolysis and divided into APHs‐1 (<3 kDa), APHs‐2 (3–10 kDa), and APHs‐3 (>10 kDa). APHs‐1‐c, purified from APHs‐1, was proved to show the strongest antioxidant effect through activity tracking experiments. APHs‐1‐c also can protect HepG2 cells against oxidative damage by reducing intracellular reactive oxygen species and MDA levels, elevating glutathione levels as well as increasing superoxide dismutase and catalase activities, and its protective effect is related to the Keap1–Nrf2 signaling pathway. Forty bioactive peptide sequences were identified from APHs‐1‐c, and Thr‐Glu‐Asp‐Asp‐Trp‐Arg‐Trp‐His (TEDDWRWH; P1), Trp‐Tyr‐Asp‐Asn‐Glu‐Trp‐Gly‐Tyr‐Arg (WYDNEWGYR; P2), and Ala‐Glu‐Asp‐His‐Glu‐Trp‐Trp‐Arg (AEDHEWWWR; P3) had the most potential for antioxidant activity by molecular docking studies. P1, P2, and P3 showed desirable 1,1‐diphenyl‐2‐picrylhydrazyl radical scavenging activity (IC50 = 0.634–3.381 mmol/L), 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt radical scavenging activity (IC50 = 0.221–0.309 mmol/L), and ferric‐reducing antioxidant power. These results suggested that almonds protein hydrolysates and its derived antioxidant peptides could serve as potential ingredients applied in functional foods.

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Antioxidant Properties and Transepithelial Transportation of Di‐/tripeptides Derived from Simulated Gastrointestinal Digestion of Pig Blood Cells Hydrolysates

Cited by 7 publications

References 35 publications

Oral Administration of Oyster Peptide Prevents Bone Loss in Ovariectomized Mice

Oral Administration of Oyster Peptide Prevents Bone Loss in Ovariectomized Mice

The modulation effects of plant‐derived bioactive ingredients on chronic kidney disease: Focus on the gut–kidney axis

Identification of potential antioxidant peptides from protein hydrolysates of pearl oil apricot almonds: Combination of in vitro and molecular docking studies

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