Antioxidant properties and phenolic content of different floral origin honeys

Piljac-Žegarac, Jasenka

doi:10.3896/ibra.4.01.2.04

Cited by 81 publications

(64 citation statements)

References 24 publications

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“…However, a study by Khalil and et al (2012) found a low correlation between phenols and reducing power using the FRAP assay. Others have reported strong correlations between the TP and FRAP assay results: r = 0.885 (Beretta et al, 2005), r = 0.88 (Krpan et al, 2009), and r = 0.873 (Piljac-Žegarac et al, 2009), in agreement with our findings (r TP/AAE-RedPw = 0.894). Nevertheless, these authors found a strong correlation between TP and the DPPH assay and a high correlation between the two assays with r>0.8 compared with r AAE-RedPw/AAE-DPPH = 0.597 in this study.…”

supporting

confidence: 82%

“…There was no correlation between colour and total flavonoids in this study (r colour/TF = -0.147, p>0.1), in contrast to the study by Khalil and et al (2012) which found a strong interdependence between these two variables (r = 0.968). These results show that colour can reflect, at least in part, the antioxidant activity of a honey sample, in agreement with the conclusions of Beretta and et al (2005) and Piljac-Žegarac and et al (2009). In the current study, it seemed that the flavonoids were mainly flavonolbased, as these do not absorb in the 500 -700 nm range (Tungjai et al, 2008).…”

supporting

confidence: 79%

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The Antioxidant Activity of Maltese Honey

Meinen

Camilleri

Attard

2014

Journal of Apicultural Science

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A b s t r a c t Maltese honey has been valued for its medicinal properties since ancient times, so much so that the word Malta is derived from the ancient Greek word, Melite, which means honey. in this study, Maltese honey samples (n = 30) from three harvest seasons and different floral sources were analysed to determine their antioxidant/antiradical properties. colorimetric assays were used to determine the polyphenol content, flavonoid content, and colour intensity, and these values were analysed to determine whether they correlated with radical scavenging activity as determined by bioassays. The honey samples varied in their dPPh activity (range, 3.326 -11.865 mg AeAc/100 g honey), reducing power (ec 50 range: 5.632 -14.453 mg/ml), total phenolic content (range, 8.114 -109.765 mg TAe/100 g honey), total flavonoids (range, 0.655 -212.865 mg Re/100 g honey), and colour intensity (range, 0.000 -99.581 mm Pfund). The data were subjected to factor analysis using principal components. The antioxidant properties correlated significantly with total polyphenol content and colour (p<0.0001). Principal components analysis revealed three clusters of honey corresponding to the three harvest seasons.

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supporting

confidence: 82%

supporting

confidence: 79%

The Antioxidant Activity of Maltese Honey

Meinen

Camilleri

Attard

2014

Journal of Apicultural Science

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“…The relationship between color parameters and antioxidant activity has been reported in various honeys (Beretta et al, 2005;Bertoncelj et al, 2007;Piljac-Žegarac et al, 2009). Our present results also showed that a positive linear correlation was observed between color (Pfund) and DPPH radical scavenging activity (r = 0.757).…”

Section: Resultsmentioning

confidence: 99%

“…The color of the honey samples was evaluated by vidifference between spectrophotometric absorbance at 450 and 720 nm. The results are expressed in the Pfund scale (mm/Pfund scale) (Piljac-Žegarac et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activity in Honeys of Various Floral Origins: Isolation and Identification of Antioxidants in Peppermint Honey

Kumazawa

Okuyama

Murase

et al. 2012

FSTR

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Polyphenol fractions from twenty-three honey samples of various floral origins were extracted with an Amberlite XAD-2 column, and total polyphenol content and antioxidant properties were determined. The Folin-Ciocalteu method was used to determine total polyphenol content, and antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total polyphenol content per 100 g of honey ranged from 17.5 mg (Japanese prickly-ash) to 132.8 mg (peppermint). Peppermint honey had the highest flavonoid content (48.3 mg/100 g) and exhibited the highest antioxidant activity of all samples. A positive linear correlation was observed between honey color and DPPH radical scavenging activity. p-Coumaric acid and kaempferol were isolated and identified as antioxidants from peppermint honey. Additionally, quantification of these compounds in other honey samples was carried out. Peppermint honey contained a large amount of p-coumaric acid (705 μg/100 g) and kaempferol (471 μg/100 g). The potent antioxidant activity of peppermint honey is attributable to the quantities of these compounds, especially kaempferol.

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“…On average the % RSA of all honey samples tested in this experiment is 39.1% and is equivalent to 130 mg ascorbic acid per 100 g honey sample analyzed. Piljac-Zegarac et al [37] reported that the mean scavenging potential of heterofloral Croatian honey was found to be 16.7 mg AAE/100 g. The DPPH radical scavenging capacities of Ethiopian honey samples were found to be very high compared to those Croatian honeys. Two Tualang honey types with the total polyphenols content (877 mg GAE/kg and 652 mg GAE/kg) showed the best radical scavenging properties with respect to DPPH· (81.6% and 77.3%) of the analyzed Malaysian honey samples [4].…”

Section: Determination Of Radical Scavenging Activities Of Honey and mentioning

confidence: 99%

Total phenols and antioxidant activities of natural honeys and propolis collected from different geographical regions of Ethiopia

Sime¹,

Atlabachew²,

Abshiro³

et al. 2015

Bull. Chem. Soc. Eth.

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ABSTRACT. In this study, ten honey and five propolis samples from different geographical origins were tested. Both honey and propolis samples showed high content of total phenolic compounds (330-610 mg gallic acid equivalent (GAE)/100 g honey; 365-1022 mg GAE/g ethanol extract of propolis (EEP). The total flavonoids ranged from (15.1-42.6 mg catechin equivalent (CE)/100 g for honey; to 123-74 mg CE/g for EEP. These honeybee products of Ethiopia had high total radical scavenging properties with respect to 2,2-diphenyl-1-picrylhydrazyl (DPPH); 18.1-59.8% and 48.6-87.8% for honey and EEP respectively. Furthermore, the hydroxymethylfurfural (HMF) of the honey samples was found to be low with a mean value of 4.8 mg/kg suggesting that the samples were of good quality. The antioxidant properties of the products showed a good correlation (r 2 = 0.50-0.82) with their polyphenolic contents.

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Antioxidant properties and phenolic content of different floral origin honeys

Cited by 81 publications

References 24 publications

The Antioxidant Activity of Maltese Honey

The Antioxidant Activity of Maltese Honey

Antioxidant Activity in Honeys of Various Floral Origins: Isolation and Identification of Antioxidants in Peppermint Honey

Total phenols and antioxidant activities of natural honeys and propolis collected from different geographical regions of Ethiopia

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