Antioxidant profile, propagation and cultivation of Nepeta camphorata, the endemic species of Mt Taygetos (Greece)

Darras, Anastasios I.; Spiliopoulos, Ioakim K.; Kartsonas, E.D.; Assimomitis, Panagiotis; Karras, S.

doi:10.1016/j.sajb.2020.03.009

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…The number of shoots per explant was highest (4.6 to 6.6 at average) when 8 g L -1 ZEA or BA were used (Figure 1A, B, F, G), but a significant number of these shoots (1.8 to 3.5 at average) were hyperhydric (Figure 1F, G). Longest shoots in general were produced on the Hf-medium or the media with low cytokinin concentration, while the shortest shoots (shorter than 1 cm at average) were produced when 8 mg L -1 ZEA, BA or 2iP were used (Table 2 and 3). C. cretica responded to MS medium and cytokinins in a very similar way with C. nepeta (Vlachou et al, 2019), while the endemic of Mt Taygetos (South Peloponnese, Greece) Nepeta camphorata was found to proliferate quite successfully on Hf-MS medium (Darras et al, 2020). A number of Mediterranean Lamiaceae and other xerophytes proliferated better at lower ΒΑ concentrations, 0.25 to 0.5 mg L -1 , compared to higher ones, 1.0 to 4.0 mg L -1 (Papafotiou et al, 2017), while other Lamiaceae such as Mentha piperita and Ocimum gratissimum (Saha et al, 2010(Saha et al, , 2012 were found to have best response in terms of shoot formation at higher concentration of BA (1.0 to 2.0 mg L -1 ).…”

Section: Shoot Proliferation As Affected By Cytokinin Type and Concentrationmentioning

confidence: 82%

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Vlachou

Papafotiou

Bertsouklis

2020

Not Bot Horti Agrobo

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The optimum range of temperature for germination (96-100%) of Calamintha cretica, an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L-1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L-1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L-1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L-1 naphthaleneacetic acid into the 8.0 mg L-1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L-1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L-1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).

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Section: Shoot Proliferation As Affected By Cytokinin Type and Concentrationmentioning

confidence: 82%

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Vlachou

Papafotiou

Bertsouklis

2020

Not Bot Horti Agrobo

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“…Moreover, this strategy is recognised to reduce pressure on critically endangered wild populations 20,63 . Darras et al 65 found significantly higher TPC of in vitro‐propagated plants of endemic Nepeta camphorata than wild plants, regardless of the extraction solvent used. On the contrary, Petrova et al 64 found that the content of phenolic components was significantly higher in wild Nepeta nuda plants from Bulgaria than in the in vitro‐propagated plants.…”

Section: Resultsmentioning

confidence: 99%

Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

Aradski

Pavlović

Janošević

et al. 2023

Phytochemical Analysis

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Introduction:The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.Materials and Methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzymeinhibiting effects were evaluated against four enzymes. Results:The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition. Conclusion:This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.

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“…The flower buds of the species are covered in dense hairs with a silvery sheen, being very attractive even before opening [4]. All the above morphological characteristics make evident the expediency of its inclusion in the floricultural industry for the production of potted plants, landscape plants and cut flowers, as similar shrubs of small stature [5][6][7][8][9][10][11][12][13]. E. cretica is the second species of the genus native to Greece, endemic but quite common on the island of Crete, which also has strong potential for use as a new floricultural crop [14] and has been studied as a member of plant communities for use in urban, Mediterranean, green roofs [15].…”

Section: Introductionmentioning

confidence: 99%

“…Contemporary, largescale commercial floriculture is in dire need of the introduction and utilization of novel crops, with the market seeking new species suitable for the new climatic conditions and challenges of the Anthropocene. Hence, there is great interest in native, drought-tolerant species of Mediterranean flora, and numerous studies have been published in recent years on the introduction of drought-tolerant Greek native species into professional horticulture [5,[7][8][9][10]12,13,[18][19][20][21]. Thus, E. sibthorpii could be introduced as a new alternative species with the added value of its adaptation to low levels of soil moisture.…”

Section: Introductionmentioning

confidence: 99%

Studies on Seed Germination and Micropropagation of Ebenus sibthorpii, an Endemic Shrub of Greece with Potential Ornamental Use

Bertsouklis,

Vazaka-Vodena,

Bazanis

et al. 2023

Horticulturae

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The introduction of new ornamental species is a challenge for the floriculture industry. Ebenus sibthorpii is an endemic species of Greece, with a strong ornamental potential. To the best of our knowledge, there are no studies on the in vitro propagation of this species. Therefore, the current study aimed to determine the possibility of micropropagation of Ebenus sibthorpii as a first step for its introduction into the floriculture industry. A preliminary study on the germination ability of the species was also conducted on 1/2-strength Murashige and Skoog medium (MS/2), in the range of 5–35 °C. Seeds germinated at 46–64% indifferently of temperature between the range of 10–30 °C. In vitro cultures were successfully established (77–80% explant response) from single-node explants excised from seedlings, on MS medium either hormone-free (Hf) or supplemented with 6-benzyladenine (BA) at 0.5 and 1 mg L−1. The subsequent multiplication stage involved subcultures in MS medium either Hf or supplemented with various cytokinin types and concentrations, while the combination of 0.01 mg L−1 naphthaleneacetic acid (NAA) with BA was also tested. Τhe highest multiplication indices (3.6–4.6) were observed in media containing BA at 0.1–0.5 mg L−1, regardless of NAA presence, and in those with 0.5 mg L−1 ZEA or 1 mg L−1 TDZ. The highest number of shoots were formed in TDZ media, but almost half of them did not elongate. To address this, a two-fold culture was developed, where micro-shoots produced on TDZ media were transferred to Hf, MS or MS/2 medium to elongate. Thus, the highest multiplication index (16.4) was achieved when micro-shoots from 1 mg L−1 TDZ medium were transferred to MS medium. The present study could be the basis of further exploitation and conservation of E. sibthorpii.

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Antioxidant profile, propagation and cultivation of Nepeta camphorata, the endemic species of Mt Taygetos (Greece)

Cited by 6 publications

References 25 publications

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

Studies on Seed Germination and Micropropagation of Ebenus sibthorpii, an Endemic Shrub of Greece with Potential Ornamental Use

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