Antioxidant potential of Sutherlandia frutescens and its protective effects against oxidative stress in various cell cultures

Tobwala, Shakila; Fan, Weili; Hines, Connor; Folk, William R.; Erçal, Nuran

doi:10.1186/1472-6882-14-271

Cited by 30 publications

(34 citation statements)

References 35 publications

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“…In vitro and in vivo studies with extracts prepared from the leaf and whole plant have provided evidence for its anti-proliferative, anti-viral, anti-stress, anti-diabetic, anti-inflammatory, anti-mutagenic, anti-bacterial, anti-oxidant, and immunostimulatory properties (van Wyk and Albrecht, 2008, Aboyade et al, 2014, Tobwala et al, 2014, Lei et al, 2015b). Our previous studies with microglial cells showed that ethanol extracts of Sutherlandia suppressed LPS- and IFNγ-induced ROS and NO production by inhibition of the JAK/STAT and ERK1/2 signaling pathways (Jiang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Phytochemicals and botanical extracts regulate NF-κB and Nrf2/ARE reporter activities in DI TNC1 astrocytes

Ajit

Simonyi

Zhao

et al. 2016

Neurochemistry International

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The increase in oxidative stress and inflammatory responses associated with neurodegenerative diseases has drawn considerable attention towards understanding the transcriptional signaling pathways involving NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and Nrf2 (Nuclear Factor Erythroid 2-like 2). Our recent studies with immortalized murine microglial cells (BV-2) demonstrated effects of botanical polyphenols to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) and enhance Nrf2-mediated antioxidant responses (Sun et al., 2015). In this study, an immortalized rat astrocyte (DI TNC1) cell line expressing a luciferase reporter driven by the NF-κB or the Nrf2/Antioxidant Response Element (ARE) promoter was used to assess regulation of these two pathways by phytochemiscals such as quercetin, rutin, cyanidin, cyanidin-3-O-glucoside, as well as botanical extracts from Withania somnifera (Ashwagandha), Sutherlandia frutescens (Sutherlandia) and Euterpe oleracea (Açaí). Quercetin effectively inhibited LPS-induced NF-κB reporter activity and stimulated Nrf2/ARE reporter activity in DI TNC1 astrocytes. Cyanidin and the glycosides showed similar effects but only at much higher concentrations. All three botanical extracts effectively inhibited LPS-induced NF-κB reporter activity. These extracts were capable of enhancing ARE activity by themselves and further enhanced ARE activity in the presence of LPS. Quercetin and botanical extracts induced Nrf2 and HO-1 protein expression. Interestingly, Ashwagandha extract was more active in inducing Nrf2 and HO-1 expression in DI TNC1 astrocytes as compared to Sutherlandia and Açaí extracts. In summary, this study demonstrated NF-kB and Nrf2/ARE promotor activities in DI TNC1 astrocytes, and further showed differences in ability for specific botanical polyphenols and extracts to down-regulate LPS-induced NF-kB and up-regulate the NRF2/ARE activities in these cells.

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Section: Discussionmentioning

confidence: 99%

Phytochemicals and botanical extracts regulate NF-κB and Nrf2/ARE reporter activities in DI TNC1 astrocytes

Ajit

Simonyi

Zhao

et al. 2016

Neurochemistry International

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“…Fernandes and coworkers [3] suggested that the antioxidant properties of S. frutescens contribute to its anti-inflammatory actions. Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay of acetone, aqueous, ethanol, methanol, and chloroform extracts of S. frutescens [4-6], antioxidant properties were demonstrated without direct evidence of anti-inflammatory activity. In fact, the evidence of anti-inflammatory activity in S. frutescens is fairly limited and mostly indirect.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the anti-inflammatory activity of Sutherlandia frutescens using murine macrophages

Browning

Eichen

et al. 2015

International Immunopharmacology

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Sutherlandia frutescens is a botanical widely used in southern Africa for treatment of inflammatory and other conditions. Previously, an ethanolic extract of S. frutescens (SFE) has been shown to inhibit the production of reactive oxygen species (ROS) and nitric oxide (NO) by murine neurons and a microglia cell line (BV-2 cells). In this study we sought to confirm the anti-inflammatory activities of SFE on a widely used murine macrophage cell line (i.e., RAW 264.7 cells) and primary mouse macrophages. Furthermore, experiments were conducted to investigate the anti-inflammatory activity of the flavonol and cycloartanol glycosides found in high quantities in S. frutescens. While the SFE exhibited anti-inflammatory activities upon murine macrophages similar to that reported with the microglia cell line, this effect does not appear to be mediated by sutherlandiosides or sutherlandins. In contrast, chlorophyll in our extracts appeared to be partly responsible for some of the activity observed in our macrophage-dependent screening assay.

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“…Most diseases are caused by inadequate balance between antioxidant defence and oxidant production in cells, a condition termed oxidative stress (Tobwala et al, 2014). Oxidative stress together with free radical production result with reduced amount of antioxidants in the cells.…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…Although synthetic antioxidants have shown protective effect against ROS, their use has been nullified by the safety and cytotoxicity issues (Ananthi et al, 2010;Fleita et al 2015). Most studies have evaluated the antioxidant activity exhibited by plants and marine algae in order to counteract the effects of diseases caused by oxidative stress (Huang and Wang, 2004;Lim et al, 2002;Shon et al, 2003;Takamatsu et al, 2003;Tobwala et al, 2014). Polysaccharides from different seaweeds such as Ulva lactuca From the best of author"s knowledge, antioxidant activity of brown seaweed, Sargassum incisifolium, has never been investigated previously.…”

Section: Antioxidant Activitymentioning

confidence: 99%

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Assessment of the bactericidal effect of green synthesized silver nanoparticles against a panel of infectious microorganisms

et al. 2016

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Nanoscience platform. For that I really want to say thank you to this platform for making my research programme a success. iv  To the University of the Western Cape, thank you for providing facilities to enable smooth progress of my research project.  Lastly, I would very much like to thank God for being there for me. Always guiding me in His own way, giving me strength when I was weak. I really exalt you my Lord. There

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Antioxidant potential of Sutherlandia frutescens and its protective effects against oxidative stress in various cell cultures

Cited by 30 publications

References 35 publications

Phytochemicals and botanical extracts regulate NF-κB and Nrf2/ARE reporter activities in DI TNC1 astrocytes

Phytochemicals and botanical extracts regulate NF-κB and Nrf2/ARE reporter activities in DI TNC1 astrocytes

Unveiling the anti-inflammatory activity of Sutherlandia frutescens using murine macrophages

Assessment of the bactericidal effect of green synthesized silver nanoparticles against a panel of infectious microorganisms

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