Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

Abstract

Elevated levels of reactive oxygen species (ROS) damage the internal cell components. Padina boryana, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of P. boryana were assessed against hydrogen peroxide (H2O2) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H2O2. PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs’ ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from P. boryana might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.

Potential Antioxidant Properties of Enzymatic Hydrolysates from Stichopus japonicus against Hydrogen Peroxide-Induced Oxidative Stress

“…Furthermore, it could regulate apoptotic cell cycle arrest under oxidative stress conditions. These results were consistent with previous results from Thilina et al (2020) [ 47 ].…”

The Potential of Sulfated Polysaccharides Isolated from the Brown Seaweed Ecklonia maxima in Cosmetics: Antioxidant, Anti-melanogenesis, and Photoprotective Activities

“…Jayawardena et al (2020) isolated sulfated polysaccharides from Padina boryana (PBP) and evaluated the antioxidant activity of PBP in both in vitro and in vivo models. The results indicated that PBP significantly protected Vero cells and zebrafish against oxidative stress induced by hydrogen peroxide [ 26 ]. Wang et al (2019) isolated sulfated polysaccharides from the edible seaweed Sargassum fulvellum (SFPS) and investigated the effect of SFPS on AAPH-induced oxidative stress.…”

“…The FT-IR spectra of EMC and the commercial fucoidan were analyzed using an FT-IR spectrometer (Bruker, Karlsruhe, Germany) according to a previously described protocol [26].…”

“…Thus, the inhibition of oxidative stress is thought to be a strategy to prevent these diseases as well as against aging. Various studies have reported that the sulfated polysaccharides isolated from seaweeds have antioxidant properties [13,14,26,42]. Wang et al (2020) isolated the sulfate polysaccharides (CFPS) from the enzymatic digest of Codium fragile and evaluated the antioxidant activity of CFPS [14].…”

Antioxidant Effects of Turmeric Leaf Extract against Hydrogen Peroxide-Induced Oxidative Stress In Vitro in Vero Cells and In Vivo in Zebrafish

“…This study focused on the cellular H 2 O 2 stimulation under both in vivo and in vitro condition as a distinct ROS species. H 2 O 2 is an important metabolite in redox signaling, redox regulation, and oxidative stress-mediated cell death, where it acts as a messenger molecule [ 38 , 39 ]. H 2 O 2 , a stable molecule, diffuses across the cellular membrane with a specific carrier and is converted into a highly reactive hydroxyl radical [ 40 ].…”