Antioxidant Potential of Resveratrol as the Result of Radiation Exposition

Abstract

The purpose of this study was to determine the effect of electron beam irradiation (EBI) at a dose of 25 kGy on the stability and antioxidant properties of resveratrol (RSV), a nutraceutical with clinically proven activity. The electron paramagnetic resonance (EPR) method was used to evaluate the concentration of free radicals after irradiation. Minor changes in chemical structure due to free radicals induced by EBI were confirmed by FTIR spectroscopy. HPLC and HPLC-MS analysis ruled out the appearance of degradation products after irradiation. In addition, HPLC analysis confirmed the absence of trans- to cis-resveratrol conversion. Changes in the antioxidant potential of RSV after irradiation were studied using DPPH, ABTS, CUPRAC, and FRAP techniques. It was confirmed that EBI favorably affected the antioxidant properties of tests based on the HAT mechanism (increase in DPPH and CUPRAC tests).

Do Rutin and Quercetin Retain Their Structure and Radical Scavenging Activity after Exposure to Radiation?

“…The total concentration of free radicals C tot (t) at any time t after irradiation was described by Equation (1) [ 28 ]: where C s —the concentration of stable radicals, C u —the concentration of unstable free radicals, and T —the mean lifetime of unstable radicals. Approximation of Equation (2) to the experimental points gives the following parameters for quercetin/rutin, respectively: C tot ( t = 0) = 0.42 ± 0.13/2.42 ± 0.46 ppm, C s = 0.29 ± 0.06/0.90 ± 0.09 ppm, C u = 0.13 ± 0.07/1.52 ± 0.3 ppm.…”

“…The changes in antioxidant activity of irradiated quercetin may be attributed to the free radicals generated by electron beam radiation. For example, the influence of free radicals on the antioxidant properties of polyphenols was observed by Rosiak et al in the case of resveratrol [ 28 ]. In the case of the 25 kGy rutin, we observe changes that oscillate around the measurement error.…”

“…The concentration of free radicals was obtained by comparing the spectra of rutin and quercetin with the spectrum of a standard (Al 2 O 3 :Cr 3+ ) with a known number of unpaired electrons. A more detailed description of the procedure for determining the concentration of radicals is elsewhere [ 28 , 51 ].…”

“…A range of medications, including vitamins, antiprotozoals, antibiotics, steroids, and anticancers, have been researched in relation to pharmacological sterilization [ 22 , 23 , 24 , 25 , 26 , 27 ]. The existing literature also provides information on the effects of ionizing radiation on plant-derived compounds and extracts [ 28 , 29 , 30 , 31 , 32 ]. Different molecular properties of drugs and natural compounds affect their radiosensitivity.…”

CO-ADMINISTRATION OF RESVERATROL RESCUED LEAD-INDUCED TOXICITY INDROSOPHILA MELANOGASTER

“…Studies indicate that certain polyphenol-producing plants exhibit significant antitoxic effects on lead toxicity (Gülçin, et al, 2009;Gerszon, et aal., 2014;Xia, et al, 2017;Colica, et al, 2018;Salehi, et al, 2018;Gu, et al, 2021;Hu, et al, 2022;Rosiak, et al, 2022). Resveratrol, a natural polyphenol found in grapes, wine, peanuts, and soy, has garnered attention for its antioxidant, anti-carcinogenic, anti-inflammatory, anti-aging, and anti-genotoxic properties (Burns et al, 2002;Baur et al, 2006;Yeh et al, 2013;Zhang et al, 2020).…”