Antioxidant potential of low molecular weight fucoidans from Sargassum autumnale against H2O2-induced oxidative stress in vitro and in zebrafish models based on molecular weight changes

Lee, Hyo Geun; Jayawardena, Thilina U.; Liyanage, N.M.; Song, Kyung‐Mo; Choi, Yun‐Sang; Kang, Min‐Cheol

doi:10.1016/j.foodchem.2022.132591

Cited by 16 publications

(14 citation statements)

References 31 publications

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“…The results demonstrate that The previous results suggest that hydrogen peroxide could stimulate cell death by inducing lipid peroxidation via stimulating intracellular ROS production in zebrafish. These adverse effects could suppressed by algal polysaccharides [31]. Compared to the previous and current results, we can speculate that SF-F effectively protected zebrafish against EtOH-induced oxidative damage by suppressing death via reducing lipid peroxidation by scavenging intracellular ROS.…”

Section: Discussioncontrasting

confidence: 55%

“…Jayawardena et al have evaluated the antioxidant effect of the Padina boryana fucoidan (PBP) and the results indicate that PBP remarkably suppressed Vero cells and zebrafish damage caused by hydrogen peroxide [30]. Lee et al investigated the antioxidant activities of low-molecular-weight fucoidans isolated from the brown seaweed Sargassum autumnale [31]. The results indicate that the fucoidan isolated from S. autumnale (SAPF3) effectively scavenged free radicals and protected Vero cells and zebrafish against oxidative damage stimulated by hydrogen peroxide [31].…”

Section: Introductionmentioning

confidence: 99%

“…Lee et al investigated the antioxidant activities of low-molecular-weight fucoidans isolated from the brown seaweed Sargassum autumnale [31]. The results indicate that the fucoidan isolated from S. autumnale (SAPF3) effectively scavenged free radicals and protected Vero cells and zebrafish against oxidative damage stimulated by hydrogen peroxide [31].…”

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Sargassum fusiforme Fucoidan against Ethanol-Induced Oxidative Damage in In Vitro and In Vivo Models

Wang

Yang

et al. 2023

Polymers

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Our previous studies have evaluated the bioactivities of a fucoidan isolated from Sargassum fusiforme (SF-F). To further investigate the health benefit of SF-F, in the present study, the protective effect of SF-F against ethanol (EtOH)-induced oxidative damage has been evaluated in in vitro and in vivo models. SF-F effectively improved the viability of EtOH-treated Chang liver cells by suppressing apoptosis. In addition, the in vivo test results indicate that SF-F significantly and dose-dependently increased the survival rate of zebrafish treated with EtOH. Further research results show that this action works through decreasing cell death via reduced lipid peroxidation by scavenging intracellular reactive oxygen species in EtOH-stimulated zebrafish. These results indicate that SF-F effectively protected Chang liver cells and zebrafish against EtOH-induced oxidative damage and suggest the potential of SF-F to be used as an ingredient in the functional food industry.

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Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Sargassum fusiforme Fucoidan against Ethanol-Induced Oxidative Damage in In Vitro and In Vivo Models

Wang

Yang

et al. 2023

Polymers

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“…While the contents of polysaccharide (73.93%), L-fucose (23.02%) and sulfate (29.88%) of DF2 were higher than those of DF1, and the molecular weights of DF2 (Mw 21,292 Da) and DF1 (Mw 14,893 Da) were similar. Based on the previous study, the sulfate and L-fucose contents were also an important determinant of the antitumor activity [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Physicochemical Characterization and Antitumor Activity of Fucoidan and Its Degraded Products from Sargassum hemiphyllum (Turner) C. Agardh

et al. 2023

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Fucoidan has many biological functions, including anti-tumor activity. Additionally, it has been suggested that low-molecular-weight fucoidans have greater bioactivities. This study aimed to examine the degradation, purification, physicochemical characterization and in vitro antitumor activity of fucoidan from Sargassum hemiphyllum (Turner) C. Agardh. Fucoidan was isolated using DEAE-cellulose-52 (F1, F2), Vc-H2O2 degration, and Sepharose CL-6B gel (DF1, DF2) from crude Sargassum fucoidans. Physicochemical characteristics of four isolated fucoidans were examined using chemical and monosaccharide composition, average molecular weight (Mw), and FTIR. Furthermore, the anti-proliferative effects of purified fucoidans on human hepatocellular carcinoma cells (HepG2), human Burkitt Lymphoma cells (MCF-7), human uterine carcinoma cells (Hela) and human lung cancer cells (A549) were analyzed by MTT method. The apoptosis of HepG2 cells was detected by flow cytometry. Our data suggest that the contents of polysaccharide, L-fucose and sulfate of DF2 were the highest, which were 73.93%, 23.02% and 29.88%, respectively. DF1 has the smallest molecular weight (14,893 Da) followed by DF2 (21,292 Da). The four fractions are mainly composed of fucose, mannose and rhamnose, and the infrared spectra are similar, all of which contain polysaccharide and sulfate characteristic absorption peaks. The results of MTT assay showed that the four fractions had inhibitory effects on HepG2 and A549 in the range of 0.5–8 mg/mL, and the four fractions had strong cytotoxic effects on HepG2 cells. DF2 had the best inhibitory effect on HepG2 (IC50 = 2.2 mg/mL). In general, the antitumor activity of Sargassum fucoidans is related to the content of L-fucose, sulfate and molecular weight, and Sargassum fucoidan has the best inhibitory effect on HepG2 hepatocellular carcinoma cells. Furthermore, when compared to MCF-7, Hela, and A549 cells, Sargassum fucoidans had the best capacity to reduce the viability of human hepatocellular carcinoma cells (HepG2) and to induce cell apoptosis, proving itself to have a good potential in anti-liver cancer therapy.

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“…Some compounds, including 2,2'-Azobis(2amidinopropane) dihydrochloride (AAPH) (Ko et al, 2014), metronidazole (MTZ) (Chen et al, 2016), and hydrogen peroxide (H 2 O 2 ) (Geun Lee et al, 2022), can be used to induce oxidative stress models in zebrafish embryos or larvae. Commonly, these zebrafish models are often used to test the antioxidant strength of potential chemicals or extracts, such as EOs.…”

Section: Zebrafish Models For the Evaluation Of Anti-oxidant And Anti...mentioning

confidence: 99%

Zebrafish models for the evaluation of essential oils (EOs): A comprehensive review

Wang,

Yu,

Ding

et al. 2023

Qual. Assur. Saf. Crops Foods

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Essential oils (EOs) derived from crops, especially aromatic plants, have been well-acknowledged to provide several health benefits for humans. Zebrafish as an unprecedented tool has been widely used as an excellent vertebrate model in labs owing to its many useful characteristics. Its applications for rapidly and economically screening and identifying toxicity, safety, and bioactivity of EOs may serve to meet the rising demand for nutraceuticals, food supplements, and natural cosmetics. In this study, we summarized the research progress of zebrafish models in evaluating EOs. In addition, toxicity, safety, and various bioactivities of EOs were summarized using a wide variety of readily applicable zebrafish models covering antioxidant, anti-inflammatory, angiogenesis inhibition, neuromodulation, anesthesia, anti-melanogenesis, analgesic activities, etc. In conclusion, zebrafish is a valuable animal model for evaluating the bioactivity and safety of EOs, and using such a model may contribute to speeding up the identification of novel EOs with potential health functions and quality assessment, which in turn boosts the recognition of aromatic plants as important industrial crops and encourages a healthier way of life.

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Antioxidant potential of low molecular weight fucoidans from Sargassum autumnale against H2O2-induced oxidative stress in vitro and in zebrafish models based on molecular weight changes

Cited by 16 publications

References 31 publications

Protective Effect of Sargassum fusiforme Fucoidan against Ethanol-Induced Oxidative Damage in In Vitro and In Vivo Models

Protective Effect of Sargassum fusiforme Fucoidan against Ethanol-Induced Oxidative Damage in In Vitro and In Vivo Models

Physicochemical Characterization and Antitumor Activity of Fucoidan and Its Degraded Products from Sargassum hemiphyllum (Turner) C. Agardh

Zebrafish models for the evaluation of essential oils (EOs): A comprehensive review

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