Antioxidant Potential in Different Parts and Callus ofGynura procumbensand Different Parts ofGynura bicolor

Abstract

Plants from Gynura family was used in this study, namely, Gynura procumbens and Gynura bicolor. Gynura procumbens is well known for its various medicinal properties such as antihyperglycaemic, antihyperlipidaemic, and antiulcerogenic; meanwhile, G. bicolor remains unexploited. Several nonenzymatic antioxidants methods were utilized to study the antioxidant capacity, which include ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, total flavonoid content, total phenolic content, and ascorbic acid content determination. DPPH assay reveals G. procumbens shoot as the lowest (66.885%) and G. procumbens root as the highest (93.499%) DPPH radical inhibitor. In FRAP assay, reducing power was not detected in G. procumbens leaf callus (0.000 TEAC mg/g FW) whereby G. procumbens root exhibits the highest (1.103 TEAC mg/g FW) ferric reducing power. Total phenolic content and total flavonoid content exhibited similar trend for both the intact plants analysed. In all antioxidant assays, G. procumbens callus culture exhibits very low antioxidant activity. However, G. procumbens root exhibited highest phenolic content, flavonoid content, and ascorbic acid content with 4.957 TEAC mg/g FW, 543.529 QE µg/g FW, and 54.723 µg/g FW, respectively. This study reveals that G. procumbens root extract is a good source of natural antioxidant.

Holistic protocol for callus culture optimization using statistical modelling

“…The results follow the same pattern found in (AA%) suggesting a correlation between both responses that has been quoted in recent researches about higher AA% related with TP (Zimmer et al 2012;Ramkissoon et al 2013). Krishnan et al (2015) also reported for Gynura procumbens higher TP concentration in root callus than those values from other plant part callus (leaf with 0.483, stem with 0.559, and root with 0.891 mg GAE g −1 Fresh Weight).…”

Gynura procumbens: An Overview of the Biological Activities

“…In addition, the administration of methanol extract prior to oxidative stress induction was able to reverse the elevation of plasma lipid peroxidation in tested animals (Akowuah et al, 2012). In order to differentiate the antioxidative capacity of different parts of G. procumbens , a recent study was conducted by Krishnan et al (2015). The study revealed that the root extract showed the highest antioxidant activity when compared to the other parts of the plant.…”

“…In Malay, G. procumbens is called Sambung Nyawa which means “prolongation of life” whereas in Chinese, it is called Bai Bing Cao which means “100 ailments” (Bodeker et al, 2009). This is because it has been utilized in traditional medicine both systemically and for topical application for treatment of different types of illnesses and diseases (Krishnan et al, 2015). For example, it is widely used to relieve kidney discomfort in Indonesia and people have been using it for the treatment of fever in Vietnam.…”

An Updated Phytochemical and Pharmacological Review on Gynura Procumbens

“…In addition, the administration of methanol extract before oxidative stress induction was able to reverse the elevation of plasma lipid peroxidation in tested animals [51]. Krishnan et al [58] revealed that the root extract has the highest antioxidant activity when compared to the other parts of the plant.…”