2002
DOI: 10.1016/s0887-2333(02)00030-9
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Antioxidant potential and gap junction-mediated intercellular communication as early biological markers of mercuric chloride toxicity in the MDCK cell line

Abstract: In this study, the early nephrotoxic potential of mercuric chloride (HgCl 2 ) has been evaluated in vitro, by exposing a renalderived cell system, the tubular epithelial Madin-Darby canine kidney (MDCK) cell line, to the presence of increasing HgCl 2 concentrations (0.1-100 mm) for different periods of time (from 4 to 72 h). As possible biological markers of the tubular-specific toxicity of HgCl 2 in exposed-MDCK cultures we analysed: (i) critical biochemical parameters related to oxidative stress conditions a… Show more

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Cited by 26 publications
(16 citation statements)
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“…One important mechanism could be that the lycopene in the oleoresin is having an important effect on oxidative stress in/among the cells. Several studies have indicated that oxidative stress is induced in many cell types by mercury (i.e., see Aleo et al, 2002;Han et al, 2007;Park and Park, 2007) although, to date, reports of mercury-induced oxidative stress in keratinocytes are lacking. Ongoing studies in our laboratory have indicated that there is, in fact, a mercury-induced oxidative stress in keratinocytes and that this outcome paralleled reductions in GJIC and in formation of pro-inflammatory cytokines in these cells (unpublished data).…”
Section: Discussionmentioning
confidence: 99%
“…One important mechanism could be that the lycopene in the oleoresin is having an important effect on oxidative stress in/among the cells. Several studies have indicated that oxidative stress is induced in many cell types by mercury (i.e., see Aleo et al, 2002;Han et al, 2007;Park and Park, 2007) although, to date, reports of mercury-induced oxidative stress in keratinocytes are lacking. Ongoing studies in our laboratory have indicated that there is, in fact, a mercury-induced oxidative stress in keratinocytes and that this outcome paralleled reductions in GJIC and in formation of pro-inflammatory cytokines in these cells (unpublished data).…”
Section: Discussionmentioning
confidence: 99%
“…Gap junctions, on the one hand, are subjected to the regulation of oxidative stress (1,(3)(4)(5). On the other hand, they impact cell responses to oxidative stress-initiated cell injury (2,19,32).…”
Section: Introductionmentioning
confidence: 99%
“…In this process, a shift to the former is termed as oxidative stress. Mercuric chloride (HgCl 2 ) is nephrotoxic in rats and affects the pars recta (S3 segment) of the proximal tubule (Stacchiotti et al 2003), inducing a necrosis that seems to be mediated by oxidative damage (Aleo et al 2002;Shimojo et al 2002). The oxidative stress induced by HgCl 2 is mediated by a strong affinity + cells in tissues from all HgCl 2 -treated rats relative to in Group VI control rat tissues (untreated, saline gavaged) were found.…”
Section: Discussionmentioning
confidence: 96%
“…Mercury is excreted almost exclusively via the kidney; thus, renal exposure to mercury is unavoidable and subsequent toxicity commonly evolves in the kidney (Madden & Fowler 2000). Renal mercury chloride (HgCl 2 )-induced damage has been studied using experimental models of nephropathy, showing that the toxic effects of HgCl 2 in the kidney are related to inflammatory events and/or oxidative stress (Ghielli et al 2000;Aleo et al 2002;Shimojo et al 2002;de Greef et al 2003;Stacchiotti et al 2003).…”
Section: Introductionmentioning
confidence: 99%