Antioxidant/Oxidant Balance: Application as a biomarker of the antioxidant status in plasma of ewes fed seaweed Ascophyllum nodosum and flaxseed under high ambient temperature

Ciliberti, Maria Giovanna; Soccio, Mario; Pastore, Donato; Albenzio, Marzia; Sevi, A.; Caroprese, Mariangela

doi:10.1016/j.smallrumres.2018.11.005

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…For example, Yerba Mate supplementation, a plant with high polyphenol concentration, seems to improve the redox balance of early-lactation cows, as reflected by a reduction of OSI value [54]. The inclusion of flaxseed plus Ascophyllum nodosum, rich of antioxidants, in the diet of late-lactation ewes showed a positive antioxidant/oxidant balance, suggesting this supplementation as a possible feeding strategy to mitigate the redox imbalance caused by environmental and physiological factors [55]. In our study, Sulla forage, which constitutes 100% and 65% of FSF100 and FSF65 diet, respectively, could have played an important role in the increase of plasma antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Status of Goats with Different CSN1S1 Genotypes Fed ad Libitum with Fresh and Dry Forages

et al. 2020

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Forty late-lactation Girgentana goats were used to study the effect of diets fed ad libitum and αS1-casein (CSN1S1) genotype on redox balance. The goats genotyped at CSN1S1 locus (A/A, A/F) were subjected to four feeding treatments different for percentage inclusion of dry and fresh forage: DAF100 (98% of Dry Alfalfa Forage), DAF65 (65% of Dry Alfalfa Forage), FSF100 (100% of Fresh Sulla Forage) and FSF65 (65% of Fresh Sulla Forage). Blood samples were analyzed for superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity, reactive oxygen metabolites (ROMs), biological antioxidant potential (BAP) and non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), albumin, glucose and cholesterol contents. The oxidative stress index (OSI) was calculated as percentage ratio of ROMs to BAP. Redox balance was improved by Sulla inclusion, as reflected in the lower OSI values found in FSF100 and FSF65 groups. DAF100 group displayed the highest GPX activity, while other groups exhibited the highest SOD activity. Fresh forage diets increased albumin concentration while no effect of tested factors was noted on glucose, NEFA, BHBA and cholesterol contents. The interaction diet × genotype was significant only for GPX activity. GPX and albumin were negatively correlated and were correlated positively and negatively with ROMs, respectively. Diet rather than genotype affects redox balance in dairy goats and a possible role of forage polyphenol compounds on oxidative status needs to be tested in future studies.

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Section: Discussionmentioning

confidence: 99%

Oxidative Status of Goats with Different CSN1S1 Genotypes Fed ad Libitum with Fresh and Dry Forages

et al. 2020

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“…It should be also outlined that the AOB approach has been recently applied as a biomarker of blood antioxidant status in ewes fed with Ascophyllum nodosum and flaxseed under high ambient temperature [81] and in sheep suffering foot rot and treated with ozone therapy [82], thus demonstrating its high versatility and applicability both in human and animal studies.…”

Section: Evaluation Of Human Blood Antioxidant Status After Food Imentioning

confidence: 99%

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

et al. 2018

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In the last decades, increasing demand of antioxidant-rich foods and growing interest in their putative role in prevention of degenerative diseases have promoted development of methods for measuring Antioxidant Capacity (AC). Nevertheless, most of these assays use radicals and experimental conditions far from the physiological ones, and are able to estimate only one or a few antioxidant mechanisms. On the other hand, the novel LOX/RNO and LOX–FL methods, based on secondary reactions between the soybean lipoxygenase (LOX)-1 isoenzyme and either 4-nitroso-N,N-dimethylaniline (RNO) or fluorescein (FL), may provide a more comprehensive AC evaluation. In fact, they are able to detect simultaneously many antioxidant functions (scavenging of some physiological radical species, iron ion reducing and chelating activities, inhibition of the pro-oxidant apoenzyme) and to highlight synergism among phytochemicals. They are applied to dissect antioxidant properties of several natural plant products: food-grade antioxidants, cereal and pseudocereal grains, grain-derived products, fruits. Recently, LOX–FL has been used for ex vivo AC measurements of human blood samples after short- and long-term intakes of some of these foods, and the effectiveness in improving serum antioxidant status was evaluated using the novel Antioxidant/Oxidant Balance (AOB) parameter, calculated as an AC/Peroxide Level ratio. An overview of data is presented.

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“…This statement is corroborated by the AOB (TAC/ROS-RNS) value as a measure of redox homeostasis, which does not result in any differences between the CON and HS groups, thus demonstrating the importance of monitoring both the antioxidant and oxidant status to evaluate oxidative stress exposure in growing lambs. The AOB index has been proposed as a reliable index of redox homeostasis in the plasma of heat-stressed sheep ( 18 ) and in human studies to evaluate the effects of diet on the serum antioxidant status ( 32 – 34 ), which underlines the need for a gold standard measure of the antioxidant status of sheep plasma ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

“…Redox balance parameters were assembled for the total antioxidant defenses and oxidants ratio calculation in the antioxidant oxidant balance (AOB) index, as previously applied in Ciliberti et al ( 18 ). The AOB index represents the ratio between the plasma antioxidant capacity, calculated using the TAC method, and the level of plasma oxidation expressed by the ROS/RNS concentration was measured as follows: AOB = TAC/ROS-RNS.…”

Section: Methodsmentioning

confidence: 99%

Role of hazelnut skin supplementation on plasma antioxidant status and cytokine profile in growing lambs

Ciliberti,

Santillo,

Caroprese

et al. 2024

Front. Vet. Sci.

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In this study, the effect of hazelnut skin dietary supplementation on antioxidant status and cytokine profile was evaluated in growing lambs. A total of 22 male lambs at the age of 2 months, balanced for their initial live weight (15.33 ± SD 1.79 kg), were selected and allocated into two experimental groups: the control group (CON) receiving a maize-barley-based concentrated diet, and the hazelnut group (HS) receiving supplementation with hazelnut skin (150 g/kg on the dry matter) as a maize substitute for the concentrate diet. The experiment lasted for 56 days. Peripheral blood was collected at 7, 35, and 56 days of the experiment. The free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, the total antioxidant capacity assay (TAC), the reactive oxygen species (ROS), and reactive nitrogen species (RNS) were determined in plasma. The secretion of IL-1β, IL-6, and IL-10 cytokines was also determined by ELISA. The DPPH was affected by the interaction between feeding strategy and time of sampling (p = 0.039) with a higher level of DPPH at 7 days in the HS group than the CON group. The time of sampling affected the levels of plasma TAC (p = 0.016), while the ROS/RNS levels showed a higher value in the HS group (p < 0.001), on average. The antioxidant/oxidant index, which combines the TAC and the ROS/RNS levels, was not affected by the inclusion of hazelnut skin in the diet (p = 0.394). The cytokine profile showed a lower IL-6 secretion at both 35 and 56 days than at 7 days, on average. Furthermore, the feeding treatment affected the IL-1β level, showing a lower level in the HS group than in the CON group on average. Lambs from the HS group had higher IL-10 plasma levels than the CON group at 7 days of the experiment. The present data highlight an antioxidant effect and a modulatory role in the cytokine profile of HS supplementation in growing lambs.

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Antioxidant/Oxidant Balance: Application as a biomarker of the antioxidant status in plasma of ewes fed seaweed Ascophyllum nodosum and flaxseed under high ambient temperature

Cited by 6 publications

References 33 publications

Oxidative Status of Goats with Different CSN1S1 Genotypes Fed ad Libitum with Fresh and Dry Forages

Oxidative Status of Goats with Different CSN1S1 Genotypes Fed ad Libitum with Fresh and Dry Forages

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

Role of hazelnut skin supplementation on plasma antioxidant status and cytokine profile in growing lambs

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