Antioxidant metabolism of coffee cell suspension cultures in response to cadmium

Gomes-Junior, Rui A.; Moldes, Carlos Alberto; Delite, Fabrício de Souza; Pompeu, Geórgia Bertoni; Gratão, Priscila Lupino; Mazzafera, Paulo; Lea, Peter J.; Azevedo, Ricardo Antunes

doi:10.1016/j.chemosphere.2006.04.056

Cited by 182 publications

(120 citation statements)

References 40 publications

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“…It is supported by the results obtained by Saffar et al (2009) peroxidase and polyphenyl oxidase activities increased in Arabidopsis thaliana seedlings with increasing cadmium concentrations when compared to the untreated plants. Moreover, the increase in CAT activity as found herein was also observed by Gomes-Júnior et al (2006) in Coffea arabica under Cd-stress conditions. This increase suggests a compensatory mechanism of defense against oxidative stress caused by toxic metal concentrations and can be explained by increase in its substrate to maintain the level of H 2 O 2 as an adaptive mechanism of the plants.…”

Section: Resultssupporting

confidence: 85%

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

El-Beltagi

Mohamed

2013

Not Bot Hort Agrobot Cluj

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The present investigation was carried out to study the role of calcium chloride in enhancing tolerance and reducing cadmium toxicity in pea seedlings. Some treatment with 1 and 5 mM CaCl 2 mitigated cadmium stress by increasing antioxidant enzyme activities: catalase (CAT), peroxidase (POD) and polyphenol oxidase (PPO), as well as by elevating contents of ascorbic acid (ASA), tocopherol and carotenoids. On the other hand, total carbohydrate and total soluble proteins decreased with increasing cadmium concentrations in comparison with control plants. However, total phenol, total free amino acids, proline and lipid peroxidation increased with increasing concentrations of cadmium acetate. Electrophoretic studies of protein revealed that cadmium treatments alone or in combination with calcium chloride were associated with the disappearance of some bands or appearance of new bands in pea seedlings. Electrophoretic studies of α-esterase, β-esterase and acid phosphatase isozymes showed wide variations in their intensities and densities.

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Section: Resultssupporting

confidence: 85%

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

El-Beltagi

Mohamed

2013

Not Bot Hort Agrobot Cluj

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“…A similar situation was already observed in coffee cell suspensions exposed to Ni (Gomes-Junior et al 2006a) and Cd (Gomes-Junior et al 2006b). While at high metal concentrations (305 μM Cd and 225 μM Ni) there was a signifi cant increase in TBARS, at low metal concentration (30.5 μM Cd and 22.5 μM Ni) TBARS levels remained low, although changes were observed in antioxidant enzyme activities.…”

Section: Discussionsupporting

confidence: 81%

“…While at high metal concentrations (305 μM Cd and 225 μM Ni) there was a signifi cant increase in TBARS, at low metal concentration (30.5 μM Cd and 22.5 μM Ni) TBARS levels remained low, although changes were observed in antioxidant enzyme activities. According to Gomes-Junior et al (2006a, 2006b, the oxidative stress tolerance at low Cd and Ni concentrations indicated by unaltered TBARS values may be due to a rapid and signifi cant increase in the activities of some of the major antioxidant enzymes, such as APX, CAT, SOD and GR, suggesting therefore an effective protection.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8

Bottcher

Nóbile

Martins

et al. 2012

Hoehnea

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-(Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8). The effects of aluminum (Al) on the activities of antioxidant enzymes and ferritin expression were studied in cell suspension cultures of two varieties of Coffea arabica, Mundo Novo and Icatu, in medium with pH at 5.8. The cells were incubated with 300 μM Al 3+ , and the Al speciation as Al 3+ was 1.45% of the mole fraction. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) were increased in Mundo Novo, whereas glutathione reductase (GR) and guaiacol peroxidase (GPOX) activities remained unchanged. SOD, GR, and GST activities were increased in Icatu, while CAT activity was not changed, and GPOX activity decreased. The expression of two ferritin genes (CaFer1 and CaFer2) were analyzed by Real-Time PCR. Al caused a downregulation of CaFER1 expression and no changes of CaFER2 expression in both varieties. The Western blot showed no alteration in ferritin protein levels in Mundo Novo and a decrease in Icatu. The differential enzymes responses indicate that the response to Al is variety-dependent.

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“…A peroxidação lipídica foi medida em um meio contendo 0,1% de ácido tricloroacético, 0,5% de ácido tiobarbitúrico e 0,1-0,4 mg de proteína do extrato enzimatico (Gomes-Junior et al, 2006). No momento da leitura, as amostras foram centrifugadas a 20.000 rpm durante 5 minutos e a absorbância do sobrenadante foi lida a 534 nm.…”

Section: Materials E Métodounclassified

Efeito fitotóxico do óleo volátil de Cleome guianensis Aubl. sobre o crescimento inicial de Senna occidentalis L.

Ventura

Silva

Simionatto

et al. 2016

Rev. bras. plantas med.

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RESUMO:Aleloquímicos são substâncias liberadas por certas espécies vegetais no ambiente que influenciam a germinação e o desenvolvimento de outras plantas. O objetivo desse estudo foi avaliar o potencial fitotóxico de Cleome guianensis Aubl., bem como determinar o grau de estresse oxidativo causado em Senna occidentalis L. Para isso, o óleo das folhas de C. guianensis, nas concentrações 0,5 µg/mL, 1,0 µg/mL, 2,5 µg/mL e 5,0 µg/mL, em solução de 5 ml foi testado em bioensaios de germinação e de crescimento da raiz primária e do hipocótilo de S. occidentalis. Os indivíduos de S. occidentalis foram macerados, homogeneizados e submetidos a ensaios de quantificação da catalase, peroxidase, SOD e de peroxidação lipídica. Os resultados indicam que todas as concentrações do óleo volátil causaram um efeito negativo sobre a germinação e crescimento de S. occidentalis. Na concentração 5,0 µg/mL, houve 56% de inibição da germinação e 83% de inibição de crescimento do hipocótilo. Ainda, o óleo aumentou a atividade das enzimas induzidas pelo estresse oxidativo catalase, peroxidase e superóxido dismutase (SOD). Quanto à peroxidação lipídica, o óleo nas concentrações 1,0 µg/ mL, 2,5 µg/mL e 5,0 µg/mL aumentou a produção de malondialdeído. Sendo assim, conclui-se que C. guianensis apresenta aleloquímicos que influenciam na germinação e no crescimento de S. occidentalis, além de aumentar a atividade das enzimas catalase, peroxidase, SOD e a produção de malondialdeído em S. occidentalis. Dessa forma, sugere-se a realização de estudos sobre o perfil químico do óleo a fim de descobrir as substâncias responsáveis por tais resultados e consolidar o potencial fitotóxico de C. guianensis.Palavras-chave: Alelopatia, bioherbicida, estresse oxidativo.ABSTRACT: Phytotoxic effect of Cleome guianensis Aubl. on the initial growth of Senna occidentalis L. Allelochemicals inhibit the germination and growth of other plants. The purpose of this study was to assess the phytotoxic potential of Cleome guianensis Aubl., as well as determine the degree of oxidative stress caused in Senna occidentalis L. For this purpose, oil from the leaves of C. guianensis in concentrations of 0.5 µg/mL, 1.0 µg/mL, 2.5 µg/mL, and 5.0 µg/mL were tested in bioassays on the germination and growth of S. occidentalis. Specimens of S. occidentalis were macerated, homogenized, and submitted to catalase, peroxidase, superoxide dismutase (SOD), and lipid peroxidation quantification tests. Results showed that all concentrations of the volatile oil had a negative effect on S. occidentalis germination and growth. In the 5.0 µg/mL concentration, the oil inhibited 56% of germination and 83% of hypocotyl growth. In addition, the oil increased the activity of the enzymes induced by oxidative stress: catalase, peroxidase, and superoxide dismutase SOD. For lipid peroxidation, the oil in the 1.0 µg/mL, 2.5 µg/mL and 5.0 µg/mL concentrations increased the production of malondialdehyde. In addition, C. guianensis presents allelochemicals that influence the germination and growth of S. occ...

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Antioxidant metabolism of coffee cell suspension cultures in response to cadmium

Cited by 182 publications

References 40 publications

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

Antioxidative responses of cell suspension cultures of two Coffea arabica varieties to low aluminum levels at pH 5.8

Efeito fitotóxico do óleo volátil de Cleome guianensis Aubl. sobre o crescimento inicial de Senna occidentalis L.

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