Antioxidant Mechanism of Betaine without Free Radical Scavenging Ability

Zhang, Mengmeng; Hong, Zhigang; Li, Huixian; Lai, Furao; Li, Xiaofeng; Tang, Yuqian; Tian, Mingyi; Wu, Hui

doi:10.1021/acs.jafc.6b03592

Cited by 104 publications

(70 citation statements)

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“…During erythrocyte hemolysis at 37 C, alkyl radicals are generated through decomposition by AAPH; through the contribution of oxygen, peroxyl radicals form and cause lipid peroxidation and the loss of membrane integrity. 17 Pretreatment with a gradient concentration of M w < 1 kDa DA-P fraction showed greater inhibition of hemolysis of AAPH-induced erythrocytes in a dose-response manner. The protective effect of M w < 1 kDa DA-P fraction on AAPH-induced morphological improvement in human erythrocytes was conrmed through SEM imaging, with membrane damage signicantly reduced in cells pretreated with 1.5 mg mL À1 of the <1 kDa DA-P fraction before the addition of AAPH (Fig.…”

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confidence: 88%

Detoxifying effects of ultrafiltration fractions of Dendrobium aphyllum peptides on chemical and AAPH-induced oxidative stress

Liu

2017

RSC Adv.

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Antioxidative peptides were extracted from Dendrobium aphyllum. The Lactobacillus strain was identified as Lactobacillus amylolyticus L6. Analysis of amino acid composition and HPLC-MS/MS analysis indicated a high antioxidative potential of the low molecular weight DA-P fractions which contained 21 different types of polypeptides. DA-P exhibited antioxidative abilities and the DA-P fraction with a size less than 1 kDa displayed optimal activity, as determined through DPPH and ABTS free radical scavenging assays and the reducing power assay. Human erythrocytes subjected to an 2,2 0 -azobis[2-methylpropionamidine] dihydrochloride (AAPH)-induced hemolysis assay using the DA-P fraction with the size below 1 kDa quenched the intracellular antioxidant system by inhibiting the generation of reactive oxygen species and malondialdehyde and regulating the activity of cellular antioxidative enzymes, including superoxide dismutase, catalase, and glutathione peroxidase. These results provide valuable information for the use of Dendrobium aphyllum as a functional food for Lactobacillus sp.

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confidence: 88%

Detoxifying effects of ultrafiltration fractions of Dendrobium aphyllum peptides on chemical and AAPH-induced oxidative stress

Liu

2017

RSC Adv.

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“…Thus, the more information is needed to know how GAA affects oxidant-antioxidant system to help its use as a nutritional compound under stress condition. Zhang et al (2016) reported that betaine exerts its antioxidant activity via two mechanisms. One mechanism involves scavenging ROS in cells via up-regulation of endogenous nonenzymatic antioxidant defences.…”

Section: Antioxidant Capacity and Enzyme Activitymentioning

confidence: 99%

“…Therefore, free radicals are electronegative. The electronegative outside surface of the protective membrane may repel free radicals, preventing them from damaging the cytomembrane (Zhang et al 2016).…”

Section: Antioxidant Capacity and Enzyme Activitymentioning

confidence: 99%

“…Betaine has two primary metabolic roles, including a methyl group donor and an osmolyte that assists in cellular water homeostasis. The antioxidant mechanism of betaine has been demonstrated to enhance non-enzymatic antioxidant defences via the methionine-homocysteine cycle and form a protective membrane by hydrophobicity and hydrophilicity properties of the three methyl groups and the carboxyl of betaine, respectively (Zhang et al 2016). …”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary guanidinoacetic acid and betaine supplementation on performance, blood biochemical parameters and antioxidant status of broilers subjected to cold stress

Nasiroleslami

Torki

Saki

et al. 2018

Journal of Applied Animal Research

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This study was conducted to determine the effects of dietary guanidinoacetic acid (GAA) and betaine supplementation on performance, antioxidant status and biochemical parameters of broilers subjected to cold stress. Based on a 2 × 2 factorial arrangement, 384-day-old male broiler chicks (Cobb) were randomly distributed between four experimental diets (with 8 replicates and 12 birds per replicate) included basal diet (as control) and the basal diet supplemented with 1200 mg/kg GAA; 600 mg/kg betaine and 1200 mg/kg GAA + 600 mg/kg betaine. No significant dietary effects were seen on performance, haematological and blood biochemical parameters including plasma glucose, uric acid, total antioxidant status, lactate, lactate dehydrogenase, red blood cell, haemoglobin, haematocrit and heterophil-to-lymphocyte ratios. However, malondialdehyde (MDA), and the activity of creatine kinase (CK), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were affected by the experimental diets. Compared with the other groups, betaine supplementation decreased liver MDA level and SOD activity while increased activity of liver GPx and serum CK and decreased serum level of MDA were observed in birds fed the GAA-included diet. Overall, based on the results, it seems that dietary GAA and betaine could have beneficial effects on antioxidant status of broilers subjected to cold stress. ARTICLE HISTORY

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“…The haemolysis of erythrocytes has been used extensively as an ex vivo model for studying the protective effects of antioxidants (Zhang et al, 2016). AAPH is a free radical initiator that generates free radicals by thermal decomposition.…”

Section: Erythrocyte Haemolysis Protective Effects Of Mp Hmp Gmp Anmentioning

confidence: 99%

Effects of simulated gastrointestinal digestion on the physicochemical properties, erythrocyte haemolysis inhibitory ability and chemical antioxidant activity of mulberry leaf protein and its hydrolysates

Sun

Yin

et al. 2017

Int J of Food Sci Tech

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Summary Proteins and peptides must be degraded and modified in the gastrointestinal (GI) tract prior to absorption; this process changes their physicochemical and biological properties. Mulberry leaf protein (MP) and its hydrolysates (HMP) have favourable antioxidant activities. To investigate, in vitro and separately, the effects of GI digestion and intestinal digestion on the stability of MP and HMP, we monitored the changes in secondary structures, amino acids, molecular weights and antioxidant activities. We found that MP was more hydrolysed by pepsin than by pancreatin, unlike HMP. The final digests of MP and HMP were mainly composed of polypeptides (0.5–6.5 kDa) and oligopeptides (<0.5 kDa), respectively. The GI digestion influenced MP and HMP differently; GI digestion increased the antioxidant efficiency of MP and decreased that of HMP. For the intestinal digests, the antioxidant activities of MP and HMP also differed. The 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulphonic acid) diammonium salt (ABTS) radical quenching abilities of MP and HMP at 1 mg mL−1 were comparable to or exceeded that of L‐glutathione (GSH) and 6‐hydroxy‐2,5,7,8‐tetramethychroman‐2‐carboxylic acid (trolox). Meanwhile, the erythrocyte haemolysis rates of MP, HMP and their GI products at 0.05–1.0 mg mL−1 were significantly lower than that of the 2,2′‐azobis (isobutyramidine) dihydrochloride (AAPH) control. Both MP and HMP can be used as natural antioxidants and may promote digestive health.

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Antioxidant Mechanism of Betaine without Free Radical Scavenging Ability

Cited by 104 publications

References 51 publications

Detoxifying effects of ultrafiltration fractions of Dendrobium aphyllum peptides on chemical and AAPH-induced oxidative stress

Detoxifying effects of ultrafiltration fractions of Dendrobium aphyllum peptides on chemical and AAPH-induced oxidative stress

Effects of dietary guanidinoacetic acid and betaine supplementation on performance, blood biochemical parameters and antioxidant status of broilers subjected to cold stress

Effects of simulated gastrointestinal digestion on the physicochemical properties, erythrocyte haemolysis inhibitory ability and chemical antioxidant activity of mulberry leaf protein and its hydrolysates

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