Antioxidant gene expression analysis and evaluation of total phenol content and oxygen-scavenging system in tea accessions under normal and drought stress conditions

Rahimi, Mehdi; Kordrostami, Mojtaba; Mohamadhasani, Fereshteh; Chaeikar, Sanam Safaei

doi:10.1186/s12870-021-03275-0

Cited by 19 publications

(14 citation statements)

References 63 publications

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“…In our study, five differentially expressed DHAR genes were identified and were shown to assist plants in producing ascorbic acid to enhance their antioxidant capacity. SOD, POD, and CAT enzymes are also important antioxidant enzymes for free radical scavenging in plants, and the genes regulating these enzymes were also differentially expressed in our study (Rahimi et al, 2021). Two SOD genes in our study were upregulated in T2, but most of the 10 CAT genes were downregulated in T2.…”

Section: Key Pathways and Drought Tolerance-related Genes Under Droug...supporting

confidence: 53%

Transcriptome Approach Reveals the Response Mechanism of Heimia myrtifolia (Lythraceae, Myrtales) to Drought Stress

Lin¹,

Wang²,

Wang³

et al. 2022

Front. Plant Sci.

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Drought is a major environmental condition that inhibits the development and cultivation of Heimia myrtifolia. The molecular processes of drought resistance in H. myrtifolia remain unknown, which has limited its application. In our study, transcriptome analyzes were compared across three treatment groups (CK, T1, and T2), to investigate the molecular mechanism of drought resistance. Plant leaves wilted and drooped as the duration of drought stress increased. The relative water content of the leaves declined dramatically, and relative electrolyte leakage rose progressively. Using an RNA-Seq approach, a total of 62,015 unigenes with an average length of 1730 bp were found, with 86.61% of them annotated to seven databases, and 14,272 differentially expressed genes (DEGs) were identified in drought stress. GO and KEGG enrichment analyzes of the DEGs revealed significantly enriched KEGG pathways, including photosynthesis, photosynthetic antenna proteins, plant hormone signal transduction, glutathione metabolism, and ascorbate and aldarate metabolism. Abscisic acid signal transduction was the most prevalent in the plant hormone signal transduction pathway, and other plant hormone signal transductions were also involved in the drought stress response. The transcription factors (including MYB, NAC, WRKY, and bHLH) and related differential genes on significantly enriched pathways all played important roles in the drought process, such as photosynthesis-related genes and antioxidant enzyme genes. In conclusion, this study will provide several genetic resources for further investigation of the molecular processes that will be beneficial to H. myrtifolia cultivation and breeding.

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Section: Key Pathways and Drought Tolerance-related Genes Under Droug...supporting

confidence: 53%

Transcriptome Approach Reveals the Response Mechanism of Heimia myrtifolia (Lythraceae, Myrtales) to Drought Stress

Lin¹,

Wang²,

Wang³

et al. 2022

Front. Plant Sci.

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“…•− contents in FD and HJ1 under nitrogen treatments(Figure 2), which are consistent with our previous study as well [7]. At the same time, the up-regulation of the relative expressions of these genes (CsSOD, CsPOD, CsCAT, CsAPX) was directly involved in the increased enzyme activities [17,47,49]. Furthermore, as one of the most important antioxidants in plant tissues, GSH/GSSG is essential in maintaining redox homeostasis [50,51].…”

Section: Discussionsupporting

confidence: 90%

“…Plants respond to biotic and abiotic stresses with a serials of physiological and biochemical responses [16,17]. As a typical abiotic stress, nitrogen stress can induce plant growth inhibition and serious morphological, metabolic and physiological abnormalities [18].…”

Section: Introductionmentioning

confidence: 99%

Improving Tea Quality by Balancing ROS and Antioxidant System through Appropriate Ammonium Nitrogen Application

et al. 2022

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Nitrogen is an important nutrient for the tea plant (Camellia sinensis), which profoundly affects the quality and value of tea. In this study, the variations of biochemical activities, antioxidant systems, and tea quality of two tea varieties under four levels of nitrogen fertilizers were analyzed to explore the responses of tea plants to nitrogen stress. The primary maximum photochemical efficiency of PSII(Fv/Fm), the photochemical quenching coefficient (qP) and the relative electron transport rate (rETR) decreased under nitrogen deficiency (ND) and high nitrogen treatments (HN) in tea plant. Meanwhile, the levels of reactive oxygen species (ROS) increased significantly under ND/HN treatments, and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) increased under HN/ND treatments. The relative expressions of antioxidant biosynthesis enzyme genes (CsSOD, CsPOD, CsCAT and CsAPX) were up-regulated under ND/HN treatments. Furthermore, the change trend of total free amino acid content under ND/HN treatments showed that nitrogen stress was not conducive to the accumulation of free amino acid content in tea, which may be related to the increase in ROS. This study presents a potential approach to improve tea quality by balancing ROS and antioxidant systems.

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“…Plants can integrate different stress signals and improve their chances of survival by inducing stomatal responses and the production of reactive oxygen species (ROS) and hormones ( Zhao et al, 2022 ). Oxygen-scavenging system can be used as a descriptor for identifying drought-tolerant accessions ( Rahimi et al, 2021 ). The analysis of transcriptome results also found that moderate drought had a great impact on the expression of Pro-, SOD-, and POD-related genes in tea roots.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling to the Effects of Drought Stress on Different Propagation Modes of Tea Plant (Camellia sinensis)

Zhou

Jiang

2022

Front. Genet.

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Tea plant (Camellia sinensis) is an important economic beverage crop. Drought stress seriously affects the growth and development of tea plant and the accumulation of metabolites, as well as the production, processing, yield and quality of tea. Therefore, it is necessary to understand the reaction mechanism of tea plant under drought conditions and find efficient control methods. Based on transcriptome sequencing technology, this study studied the difference of metabolic level between sexual and asexual tea plants under drought stress. In this study, there were multiple levels of up-regulation and down-regulation of differential genes related to cell composition, molecular function and biological processes. Transcriptomic data show that the metabolism of tea plants with different propagation modes of QC and ZZ is different under drought conditions. In the expression difference statistics, it can be seen that the differential genes of QC are significantly more than ZZ; GO enrichment analysis also found that although differential genes in biological process are mainly enriched in the three pathways of metabolic, single organism process and cellular process, cellular component is mainly enriched in cell, cell part, membrane, and molecular function, and binding, catalytic activity, and transporter activity; the enrichment order of differential genes in these pathways is different in QC and ZZ. This difference is caused by the way of reproduction. The further study of these differential genes will lay a foundation for the cultivation methods and biotechnology breeding to improve the quality of tea.

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Antioxidant gene expression analysis and evaluation of total phenol content and oxygen-scavenging system in tea accessions under normal and drought stress conditions

Cited by 19 publications

References 63 publications

Transcriptome Approach Reveals the Response Mechanism of Heimia myrtifolia (Lythraceae, Myrtales) to Drought Stress

Transcriptome Approach Reveals the Response Mechanism of Heimia myrtifolia (Lythraceae, Myrtales) to Drought Stress

Improving Tea Quality by Balancing ROS and Antioxidant System through Appropriate Ammonium Nitrogen Application

Transcriptome Profiling to the Effects of Drought Stress on Different Propagation Modes of Tea Plant (Camellia sinensis)

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