Antioxidant Enzymes and Tissue Regeneration in Eurythoe complanata (Polychaeta: Amphinomidae) Exposed to Used Vehicle Crankcase Oil

Abstract

Polychaetes, Eurythoe complanata, from the Gulf of Cariaco,Venezuela, were exposed to 0.3, 1.6, and 3.3% water-soluble fraction (WSF) of used crankcase oil during 15 and 21 days. The antioxidant enzymes glutathione peroxidase (GPX), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferase (GST) were assayed in the body wall tissue. Furthermore, after chemical exposure, the polychaetes were cut into equal halves; then wound healing and the number of regenerated body segments were recorded periodically. GST activity was affected by all the experimental treatments, with activity increasing with WSF concentrations. GPx activity was altered for the contamination period. GR and CAT activities rose in response to increasing WSF concentrations, and were higher for long-term than for short-term exposures. The wound healing of the transected body regions was retarded by WSF exposure. WSF affected the tissue regeneration, which was almost abolished at 3.3% WSF. The exposure period did not affect the tissue-repairing responses. Alteration of GST in contaminated organisms suggested equivalent changes in detoxication of bioaccumulated organic contaminants. The variation of GR and CAT suggests induction of oxidative stress that could reduce the ability of WSF-exposed worms to repair damaged tissue.

Acute sulphide toxicity in Perinereis aibuhitensis under different salinities and temperatures: LC50 and antioxidant responses

“…A similar result was also found when P. aibuhitensis was exposed to Pb 2+ (Tian et al 2014). Previous studies have suggested that CAT activity increases when living organisms suffer toxic oxidative stress (Nusetti et al 2005, Tian et al 2014). In our study, increased CAT activity was found at 12 h of exposure, which possibly counterbalanced sulphide-induced ROS production.…”

“…Therefore, we inferred that SOD neutralized ROS instead of CAT at this time. Nusetti et al (2005) also found that the existence of other enzymatic and nonenzymatic antioxidants played a function in stead of GSH activity, which could be influenced by glutathione reductase (GR) activity. CAT activity in all treatments was lower than that in the control during the initial 6 h of the recovery period, which seemed to be an extension of those at 96 h exposure.…”

Variaciones temporales de las defensas antioxidantes e índices proteína:ADN y ARN:ADN en el poliqueto Eurythoe complanata

“…Sin embargo, en el músculo blanco del pez Mullus barbatus, las enzimas SOD y GST incrementan sus actividades en invierno, y CAT, GR y GPx en primavera (Mathieu et al 1991, Pavloviae et al 2009). Existe complejidad en la interpretación de la actividad de las enzimas antioxidantes, ya que también pueden observarse niveles elevados en presencia de contaminantes (Nusetti et al 2005, Zapata-Vívenes et al 2005.…”

“…E. complanata es una especie que ha sido utilizada como modelo biológico en la estandarización de protocolos ecotoxicológicos, a través de biomarcadores moleculares y celulares (sistema inmune y antioxidante, poder de regeneración de tejidos, y la capacidad corporal de acumular xenobióticos) aplicados a la evaluación de sedimentos marino-costeros impactados por contaminantes (Méndez & Páez-Osuna 1998, Nusetti et al 1998, 2005Zapata-Vívenes et al 2005, Vásquez-Núñez et al 2007. Sin embargo, no existe información sobre sus respuestas bioquímicas basales mediadas por los cambios ambientales, en especialmente la temperatura.…”

Regeneration in spiralians: evolutionary patterns and developmental processes

“…Following removal of only the asegmental region, regeneration of the prostomium has been documented in several species of Erséus and Källersjö (2004), Struck et al (2007), Struck et al (2011), andWeigert et al (2014). Regeneration data are from Morgulis (1909), Cresp (1952), Olive and Moore (1975), Nusetti et al (2005), Hentschel and Harper (2006), and Giani et al (2011) or are reviewed in Hyman (1940), Berrill (1952, Herlant-Meewis (1964), Bely (2006Bely ( , 2010. AR -anterior regeneration; PR -posterior regeneration. naidid clitellates (including one that cannot regenerate segments), but fails to occur in at least one species of naidid, two species of dorvilleid, and one species of nereid (Pfannenstiel, 1973(Pfannenstiel, , 1974Bely and Sikes, 2010a).…”