Antioxidant enzyme systems and the ascorbate–glutathione cycle as contributing factors to cadmium accumulation and tolerance in two oilseed rape cultivars (Brassica napus L.) under moderate cadmium stress

Wu, Zhichao; Zhao, Xiaohu; Sun, Xu; Tan, Qiling; Tang, Yajuan; Nie, Zhaojun; Qu, Chanjuan; Chen, Zuoxin; Hu, Chengxiao

doi:10.1016/j.chemosphere.2015.06.080

Cited by 119 publications

(57 citation statements)

References 42 publications

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“…Generally, ROS is scavenged by antioxidant enzymes. For example, SOD catalyzes O 2 – into H 2 O 2 , and then H 2 O 2 is disintegrated by CAT and APX (Wu S. et al, 2015; Wu Z. et al, 2015). Our previous studies have suggested that oxidative damage is relieved by the improvement of antioxidant defense ability by Mo in plants under cold, drought and salt stress (Sun et al, 2006; Zhang et al, 2012; Wu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Hydrogen peroxide (H 2 O 2 ) was assayed following the method of Wu Z. et al (2015). Fresh wheat leaves were sampled and immediately homogenized in 0.1% (w/v) cold trichloroacetic acid (TCA), and the homogenate was centrifuged at 12000 g for 20 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Frozen wheat leaves were used to evaluate the expression of antioxidant enzyme genes according to the method of Wu Z. et al (2015). The total RNA was dissolved in 30 μL DEPC⋅H2O, and quantified with a NanoDrop 2000 UV-VIS spectrophotometer (Thermo, United States).…”

Section: Methodsmentioning

confidence: 99%

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Nitric Oxide Mediates Molybdenum-Induced Antioxidant Defense in Wheat under Drought Stress

Tan

et al. 2017

Front. Plant Sci.

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Molybdenum (Mo) has been reported to alleviate drought stress by enhancing antioxidant defense in plants, but the underlying mechanism remains unclear. Here, we hypothesized that Mo mediates nitric oxide (NO)-induced antioxidant defense through Mo-enzymes, particularly by nitrate reductase (NR) in wheat under drought stress. The 30-day-old wheat seedlings cultivated in -Mo (0 μM Mo) and +Mo (1 μM Mo) Hoagland solutions were detached and then pretreated with Mo-enzyme inhibitors, NO scavengers, NO donors or their combinations according to demands of complementary experiment under 10% polyethylene glycol 6000 (PEG)-stimulated drought stress (PSD). Mo supplementation increased the activities and transcripts of antioxidant enzymes, decreased H2O2 and MDA contents, and elevated NO production, implying that Mo-induced antioxidant defense may be related to NO signal. Complementary experiment showed that NO production was induced by Mo, while suppressed by Mo-enzyme inhibitors and NO scavengers, but restored by NO donors, suggesting that Mo-induced increase of NO production may be due to the regulation by Mo-enzymes. Further experiment indicated that the increased activities and transcripts of antioxidant enzymes induced by Mo were suppressed by Mo-enzyme inhibitors and NO scavengers, and NO donors could eliminate their suppressing effects. Moreover, Mo application increased NR activity and inhibitors of Mo-enzymes inhibited NR activity in wheat leaves under PSD, suggesting that NR might involve in the regulation of Mo-induced NO production. These results clearly indicate that NO mediates Mo-induced antioxidant defense at least partially through the regulation of NR.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Nitric Oxide Mediates Molybdenum-Induced Antioxidant Defense in Wheat under Drought Stress

Tan

et al. 2017

Front. Plant Sci.

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“…The enzymatic mechanisms of ROS scavenging include the action of a number of enzymes, such as superoxide dismutase (SOD), which catalyzes O 2 • − into H 2 O 2 (Gratão et al 2008a). Subsequently, H 2 O 2 may be detoxified into H 2 O by ascorbate peroxidase (APX) and catalase (CAT), among other peroxidases (Wu et al 2015). The nonenzymatic antioxidants include ascorbic acid and glutathione, as well as vitamins, flavonoids, alkaloids, and carotenoids (Gratão et al 2005;Alcântara et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid-deficient sit tomato mutant responses to cadmium-induced stress

et al. 2016

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There is a very effective cross-talk between signals triggered by reactive oxygen species and hormonal responses in plants, activating proteins/enzymes likely to be involved in stress tolerance. Abscisic acid (ABA) is known as a stress hormone that takes part in the integration of signals. This work aimed to characterize the biochemical response and ultrastructural changes induced by cadmium (Cd) in the Micro-Tom (MT) sitiens ABA-deficient mutant (sit) and its wild-type (MT) counterpart. MT and sit plants were grown over a 96-h period in the presence of Cd (0, 10, and 100 μM CdCl). The overall results indicated increases in lipid peroxidation, hydrogen peroxide content and in the activities of the key antioxidant enzymes such as catalase, glutathione reductase, and ascorbate peroxidase in both genotypes. On the other hand, no alteration was observed in chlorophyll content, while the activity of another antioxidant enzyme, superoxide dismutase, remained constant or even decreased in the presence of Cd. Roots and shoots of the sit mutant and MT were analyzed by light and transmission electron microscopy in order to characterize the structural changes caused by the exposure to this metal. Cd caused a decrease in intercellular spaces in shoots and a decrease in cell size in roots of both genotypes. In leaves, Cd affected organelle shape and internal organization of the thylakoid membranes, whereas noticeable increase in the number of mitochondria and vacuoles in MT and sit roots were observed. These results add new information that should help unravel the relative importance of ABA in regulating the cell responses to stressful conditions induced by Cd apart from providing the first characterization of this mutant to oxidative stress.

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“…; Wu et al . ). Antioxidant enzymes such as SOD, POD and CAT play an important role in the detoxification of plants exposed to stress (Zhang et al .…”

Section: Discussionmentioning

confidence: 97%

Intermittent lead‐induced stress on antioxidant enzyme activity and subcellular distribution of Pb in Pogonatherum crinitum seedlings

et al. 2019

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Pogonatherum crinitum is a promising lead (Pb) hyperaccumulator due to its high Pb tolerance and accumulation ability. However, the mechanisms that support Pb accumulation and tolerance in P. crinitum are not yet clearly understood.• An indoor hydroponic experiment was conducted by cultivating P. crinitum seedlings exposed to intermittent Pb stress for 60 days, divided into four stages (T1, T2, T3 and T4), with a 15-day duration per stage. The following concentrations of Pb were used: 0, 500, 0, 500 mgÁl À1 and 0, 1000, 0, 1000 mgÁl À1 ). Antioxidant enzyme activity, Pb concentration and subcellular distribution of Pb were measured at each of the above stages.• The results showed that superoxide dismutase (SOD) activity in shoots, and SOD, peroxidase (POD) and malondialdehyde (MDA) activity in shoots and roots significantly increased from T1 (no Pb stress) to T2 (Pb stress) in both 500 mgÁl À1 and 1000 mgÁl À1 treatments; however, no significant difference was noted between stages T3 (no Pb stress) and T4 (Pb stress). There was no obvious effect of Pb stress on catalase (CAT) activity in shoots and roots among different stages. The Pb concentration in shoots was up to 5090.90 mgÁkg À1 and 7573.57 mgÁkg À1 , and the bioconcentration factor (BFC) was 10.18 and 7.57 for the 500 mgÁl À1 and 1000 mgÁl À1 treatments, respectively, which confirmed the Pb hyperaccumulator characteristics of P. crinitum. For plants under Pb stress, most of the Pb was fixed in the cell walls, with a smaller amount in leaves and root vacuoles.• Both SOD and POD scavenging of reactive oxygen radicals and fixing and compartmentalisation of Pb in the cell wall might play important roles in detoxification of P. crinitum seedlings in response to Pb stress. There was no phased response of P. crinitum to intermittent Pb stress and the physiological response to Pb stress may be contiguous.Plant Biology 21 (2019) 634-642

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Antioxidant enzyme systems and the ascorbate–glutathione cycle as contributing factors to cadmium accumulation and tolerance in two oilseed rape cultivars (Brassica napus L.) under moderate cadmium stress

Cited by 119 publications

References 42 publications

Nitric Oxide Mediates Molybdenum-Induced Antioxidant Defense in Wheat under Drought Stress

Nitric Oxide Mediates Molybdenum-Induced Antioxidant Defense in Wheat under Drought Stress

Abscisic acid-deficient sit tomato mutant responses to cadmium-induced stress

Intermittent lead‐induced stress on antioxidant enzyme activity and subcellular distribution of Pb in Pogonatherum crinitum seedlings

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