Antioxidant Enzyme and Mda Level in the Skin of Albino Rat Under the Stress of Artificial Uvb Radiation

Abstract

The aim of present study was to investigate the level of skin antioxidants and MDA in albino rats under stress of acute (1 day) and sub acute (30 days) artificial UVB exposure. Twenty healthy male albino rats (100-140 g) were taken for the present experiment. Rats were grouped into four sets (5 rats in each set). The Set A (Control set) and Set A1 (Control set) were not exposed to artificial UVB radiation while Set B was exposed to 0.44 J/cm 2 of artificial UVB radiation continuously for 10 hours for 1 day and Set B1 was exposed to 0.014 J/cm 2 of UVB radiation continuously for 20 min/day for 30 days. Results of study indicates a significant decrease in Catalase, SOD level and significant increase in MDA level in the skin of albino rats after acute and sub acute artificial UVB exposure. Present study also suggests decrease in SOD and Catalase content, while increase in MDA content in the skin of albino rats of Set B is more than the Set B1. Study concluded that the UVB radiation causes damage to the antioxidant system leads to decrease in Catalase and SOD level while induces the lipid peroxidation which increases MDA level in the skin of albino rat.