Antioxidant Enzyme Activities in Ascorbic Acid and Selenium Applied Hepatocellular Carcinoma Cells

Abstract

Ascorbic acid and selenium have potential use in the prevention of cancer. The role of antioxidant enzymes against cancer is to prevent oxidative damage. In this study, the effects of L-selenomethionine (234 µM), SeO2 (100 µM), ascorbic acid (0,313 mM, and 31,3 mM) on antioxidant enzyme activities in HepG2 cell line were studied. GPx, CAT, and SOD activities of the treated cells were measured spectrophotometrically. There was a significant increase in the activity of GPx in HepG2 cells treated with L-selenomethionine (234 µM), SeO2 (100 µM), and ascorbic acid (0,313 Mm and 31,3 mM). However, the activity of CAT was found significantly decreased in L-selenomethionine (234 µM), SeO2 (100 µM), ascorbic acid (31,3 mM) treated HepG2 cells. The SOD levels of HepG2 cells treated with ascorbic acid (0,313 mM and 31,3 mM) were found higher than the control cells. The results of the present study indicated that the antioxidant enzyme activities changed depending on L-selenomethionine, SeO2 application, and CAT activities changed depending on ascorbic acid doses.

Potassium Nitrate and Ascorbic Acid Priming Improved Tissue Chemical Composition and Antioxidant and Antimicrobial Activities of Linseed (Linum usitatissimum L.) Sprouts

“…44,45 Indeed, the increase in SOD was related to the contribution of AsA to the production of this enzyme and may produce ascorbyl radicals. 46 Furthermore, the application of AsA increases the CAT activity, and our obtained results are in line with the results of El-Bassiouny and Sadak. 47 Besides, the APX activity is linked with the chloroplast AsA pool.…”