Antioxidant Enzymatic Activity and Its Related Genes Expression in Cassava Leaves at Different Growth Stages Play Key Roles in Sustaining Yield and Drought Tolerance Under Moisture Stress

Zhu, Yanmei; Luo, Xiangrong; Wei, Mingdong; Khan, Aziz; Munsif, Fazal; Huang, Tangwei; Pan, Xiaolu; Shan, Zhongying

doi:10.1007/s00344-019-10003-4

Cited by 6 publications

(10 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Long time adaptation in glycerate conditions might improve the antioxidant defense system. Five genes related to antioxidant defense system including superoxide dismutase (SOD), catalase (katA), glutathione peroxidase (GSH-px), alkyl hydroperoxide reductase (ahpF) and DNA binding protein (mrgA), were compared to explore the effect of adaption on cell defense and MK-7 production (Zhu et al 2020 ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator

Zhang

Peng

et al. 2022

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Menaquinone-7 (MK-7) is a kind of vitamin K2 playing an important role in the treatment and prevention of cardiovascular disease, osteoporosis and arterial calcification. The purpose of this study is to establish an adaptive evolution strategy based on a chemical modulator to improve MK-7 biosynthesis in Bacillus natto. The inhibitor of 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase), glyphosate, was chosen as the chemical modulator to perform the experiments. The final strain ALE-25–40, which was obtained after 40 cycles in 25 mmol/L glyphosate, showed a maximal MK-7 titer of 62 mg/L and MK-7 productivity of 0.42 mg/(L h), representing 2.5 and 3 times the original strain, respectively. Moreover, ALE-25–40 generated fewer spores and showed a higher NADH and redox potential. Furthermore, the mechanism related to the improved performance of ALE-25–40 was investigated by comparative transcriptomics analysis. Genes related to the sporation formation were down-regulated. In addition, several genes related to NADH formation were also up-regulated. This strategy proposed here may provide a new and alternative directive for the industrial production of vitamin K2. Graphical Abstract

show abstract

Section: Resultsmentioning

confidence: 99%

Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator

Zhang

Peng

et al. 2022

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…Drought stress not only triggers physiological and biochemical regulation but also regulates gene expression at the molecular level. There is a positive relationship between antioxidants enzyme-related gene expression and drought tolerance (Zhu et al 2020a(Zhu et al , 2020b. In this study, genes related to CAT, APX, and GR enzymes are evaluated in control and drought-hardened plants in H and Y variety seedlings (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

“…The activity of APX and GR are correlated with APX1 and GR2 transcripts levels in drought‐hardening treatments in the H variety only, while the activities and the expression levels are not consistent with each other in the Y cultivar. The inconsistencies between the enzyme activities and its transcripts levels can be explained by isogene specificity (Furlan et al 2014), and the enzyme activity changes are regulated by the posttranscriptional level which in part might result in enzyme inactivation or degradation (Zhu et al 2020a, 2020b). The above results suggest that circular drought‐hardening enhance the activities of antioxidant enzyme and their gene transcript levels which might play a decisive role in drought tolerance by alleviating the ROS cellular damage.…”

Section: Discussionmentioning

confidence: 99%

Circular drought‐hardening confers drought tolerance via modulation of the antioxidant defense system, osmoregulation, and gene expression in tobacco

Khan

Zhang

et al. 2021

Physiologia Plantarum

View full text Add to dashboard Cite

Drought stress hinders the growth and development of crop plants and ultimately its productivity. It is expected that drought stress will be frequent and intense in the future due to drastic changes in the global climate. It is necessary to make crop plants more resilient to drought stress through various techniques; drought-hardening is one of them. Defining various metabolic strategies used by tobacco plants to confer drought tolerance will be important for maintaining plant physiological functions, but studies addressing this topic are limited. This study was designed to elucidate the drought tolerance and adaptation strategies used by tobacco plants via the application of different circular drought-hardening cycles (control: no drought-hardening, T1: one cycle of drought hardening, T2: two cycles of drought-hardening, and T3: three cycles of drought-hardening) to two tobacco varieties namely Honghuadajinyuan (H) and Yun Yan-100 (Y). The results revealed that drought-hardening decreased the fresh and dry biomass of the tobacco plants. The decrease was more pronounced in the T3 treatment for both H (23 and 29%, respectively) and Y (26 and 31%, respectively) under drought stress. The MDA contents, especially in T1 and T2 in both varieties, were statistically similar compared with control under drought stress. Similarly, higher POD, APX, and GR activities were observed, especially in T3, and elevated amounts of AsA and GSH were also observed among the different circular drought-hardening treatments under drought stress. Thus circular droughthardening mitigated the oxidative damage by increasing the antioxidant enzyme activities and elevated the content of antioxidant substances, a key metabolic strategy under drought stress. Similarly, another important plant metabolic strategy is the osmotic adjustment. Different circular drought-hardening treatments improved the accumulation of proline and soluble sugars contents which contributed to osmoregulation. Finally, at the molecular level, circular drought-hardening improved the transcript levels of antioxidant enzyme-related genes (CAT, APX1, and GR2), proline and polyamines biosynthesis-related genes (P5CS1 and ADC2), and ABA signaling (SnRK2), and transcription factors (AREB1 and WRKY6) in response to drought stress. As a result, circular drought-hardening (T2 and T3 treatments) promoted tolerance to water stress via affecting the anti-oxidative capacity, osmotic adjustment, and regulation of gene expression in tobacco.

show abstract

“…Under drought conditions (Zhu et al, 2020), the expression of 13 and nine MeGATA genes was induced and reduced in treated leaf samples (Figure 4). Among them, four genes, namely MeGATA08, 10, 18, and 23 were highly up-regulated, by 240.…”

Section: Expression Analysis Of the Megata Genes Responding To Variou...mentioning

confidence: 99%

“…The fragments per kilobase of transcript per million reads mapped (FPKM) value was used to represent the expression profile of each gene (Wilson et al, 2017). Next, three transcriptome atlas, related to biotic stress, particularly CBSD inoculation (GSE56467) as previously reported (Maruthi et al, 2014), and abiotic stress conditions, including polyethylene glycol 6000 treatment (GSE93098) (Ding et al, 2017) and drought stress (GSE98537) (Zhu et al, 2020) were re-analyzed. Finally, the heatmaps of the GATA gene's expression were then clustered by R script.…”

Section: Re-analysis Of Expression Patterns Of Genes Encoding the Gat...mentioning

confidence: 99%

GENOME-WIDE IDENTIFICATION AND CHARACTERIZATION OF THE GATA TRANSCRIPTION FACTOR FAMILY SUGGESTS FUNCTIONAL EXPRESSION PATTERN AGAINST VARIOUS ENVIRONMENTAL CONDITIONS IN CASSAVA (Manihot esculenta)

2024

THE JAPS

View full text Add to dashboard Cite

GATA transcription factors (TFs) play a significant role in regulating many plant physiological processes. The GATA TF family has been identified and characterized in many important crop species. However, no information is available on the GATA TFs in cassava (Manihot esculenta). In this study, 36 MeGATA genes have been comprehensively identified, annotated, and characterized in the cassava genome using various bioinformatics tools. The gene structure and duplication of the MeGATA genes indicated the redundancy and differences in their gene structural organization. The GATA TFs in cassava could divide into three different groups, as in other plant species. Interestingly, the expression levels of the MeGATA genes were significantly changed in various major organs/tissues in the growth and development, especially in response to adverse environmental conditions. Taken together, this study could propose a list of candidate genes for further functional characterization of stress-inducible MeGATA genes in cassava.

show abstract

Antioxidant Enzymatic Activity and Its Related Genes Expression in Cassava Leaves at Different Growth Stages Play Key Roles in Sustaining Yield and Drought Tolerance Under Moisture Stress

Cited by 6 publications

References 54 publications

Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator

Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator

Circular drought‐hardening confers drought tolerance via modulation of the antioxidant defense system, osmoregulation, and gene expression in tobacco

GENOME-WIDE IDENTIFICATION AND CHARACTERIZATION OF THE GATA TRANSCRIPTION FACTOR FAMILY SUGGESTS FUNCTIONAL EXPRESSION PATTERN AGAINST VARIOUS ENVIRONMENTAL CONDITIONS IN CASSAVA (Manihot esculenta)

Contact Info

Product

Resources

About