Antioxidant efficacy of flavonoid-rich fraction from Spermacoce hispida in hyperlipidemic rats

Kaviarasan, Kuppusamy; Kalaiarasi, Panneerselvam; Pugalendi, Viswanathan

doi:10.32725/jab.2008.020

Cited by 23 publications

(15 citation statements)

References 27 publications

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“…These cells produce antioxidants such as GPx to protect against ROS (Sadeghi et al 2008). ii) Sr was reported to be an effective scavenger for oxidative radicals and this antioxidant activity protects from damage and death (Kaviarasan et al 2008 ions like silica, calcium, phosphate, and sodium ions that contribute to a balanced oxidative status. That's why BG-Sr was more efficient than BG.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats

et al. 2012

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International audienceRecently, oxidative stress has been identified as a pivotal pathological factor inducing bone osteoporosis. This phenomenon is responsible for low bone density. It alters bone quality and generates bone fractures. Strontium is found to induce osteoblast activity by stimulating bone formation and reducing bone resorption by restraining osteoclasts. Bioglass (BG) has been used to repair bone defects, and, in combination with strontium (BG-Sr), offers an opportunity to treat this disease. This study investigated the potential role of BG-Sr in improving antioxidant activity and regenerative bone capacity, The effects of both BG-Sr and BG were tested on osteoblast SaOS2 and endothelial EAhy926 cell proliferation in vitro. In vivo, BG-Sr and BG were implanted in the femoral condyles of Wistar rats and compared to that of control groups. Cell proliferation increased significantly by 120% at SaOS2 and 127% at EAhy926. Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) were significantly enhanced in BG-Sr treated rats compared to other groups. Moreover, a significant decrease of thiobarbituric acid-reactive substances (TBARs) was observed. The Ca/P ratio increase improved progressive bone mineralization. According to these results, BG-Sr ameliorated cell proliferation and developed an antioxidative defense against ROS. The histological findings highlight the BG-Sr implications in the osteoporosis treatment confirmed by bone construction. The development of BG-Sr as a therapeutic biomaterial protecting against oxidative stress might make an effective choice for application in tissue engineering

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Section: Discussionmentioning

confidence: 99%

Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats

et al. 2012

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“…This high concentration obviously has a chance to undergo LDL oxidation and tocopherol‐mediated peroxidation because it enters into the artery wall (Osterud and Bjorklid 2003). Previous studies from our laboratory have showed that S‐Frf has antioxidant and antilipidperoxidative activity in HFD rats (Kaviarasan et al . 2008).…”

Section: Resultsmentioning

confidence: 94%

“…The two fractions were negative for polyphenols. Then, the defatted bulk residue was successively extracted by refluxing with ethyl acetate (1:3 w/v) (15 h twice) (Kaviarasan et al . 2008).…”

Section: Methodsmentioning

confidence: 99%

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Antihyperlipidemic Efficacy of Flavonoid-Rich Fraction From Spermacoce Hispida Seed on High-Fat-Diet Fed Rats: A Dose-Dependent Study

Kaviarasan

Mohankumar²,

Narasimhan³

et al. 2011

Journal of Food Biochemistry

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The aim of the experiment was to investigate the antihyperlipidemic effect of Spermacoce hispida seed-flavonoid-rich fraction by a dose-dependent study. Lipid and lipoprotein profiles and the activities of lipid metabolizing enzymes were measured in plasma and tissues of high-fat-diet fed and control rats. The levels of plasma and tissue total cholesterol, triglycerides and plasma phospholipids, free fatty acids, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol were reduced significantly, while high-density lipoprotein cholesterol significantly elevated in S. hispida flavonoid-rich fraction (S-Frf) fed groups when compared to high-fat-diet fed group. HMG CoA reductase activity significantly decreased while the lipoprotein lipase and lecithin cholesterolacyl transferase activities were elevated in rats fed with flavonoid-rich fraction. Among the doses, 40 mg dose showed maximum effect. High performance liquid chromatography analysis of flavonoidrich fraction indicates that the antihyperlipidemic activity is due to the presence of ursolic acid, quercetin, isorhamnetin and hesperetin. PRACTICAL APPLICATIONSSpermacoce hispida Linn (Rubiaceae) was popularly known as "Nattaiccuri" in Tamil or "Shaggy button weed" in English. It is richly distributed in the Western ghats of Kerala state and in the Maruthamalai forest, which is an extension of Western ghats in Tamil Nadu, India. It has also been used as a substitute for coffee. It has also been reported that S. hispida contains the maximum percent of flavonoids among 25 plants analyzed. The results demonstrated that the flavonoid-rich fraction from S. hispida seed decreased the levels of lipids in the plasma and tissues, though little less than atorvastatin, which is supported by histological studies. Hence, the compounds present in S. hispida seed offer promising potential remedy that may be used to reduces the risk in hyperlipidemic associated cardiovascular complications.

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“…Plant extracts and plant enriched sources especially are considered suitable and easily available products for the prevention of these pathologies, as reported by e.g. Kaviarasan et al (2008).…”

Section: Introductionmentioning

confidence: 99%

Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

Pohanka¹,

Sochor²,

Ruttkay-Nedecký³

et al. 2012

J Appl Biomed

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In the food industry, in the process of creating new agricultural plant products, and in the testing of anti-cancer drugs there is often a need to assay multiple samples of low molecular weight antioxidants, plant samples and foods rich in antioxidants, with minimal additional costs and low degrees of uncertainty. With these demands in mind, we decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and application of reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes. Five methods were introduced for the automation: 2-diphenyl-1-picrylhydrazyl (DPPH) test, ferric reducing antioxidant power (FRAP) method, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) based test, N,N-dimethyl-1,4-diaminobenzene (DMPD) based test and the free radicals method. Samples containing one of the four antioxidants (standard rutin, quercitrin, ferulic and gallic acid) in a range 1-1000 μg/ml were used throughout. All of the tested methods were found suitable for implementation in an automated assay. However, some of them, such as the ABTS test failed to assay all tested antioxidants. The coefficients of determination were also unequal. From the analytical point of view, FRAP methods provided the most reliable results in the automated assay; because of the capacity of the method, approximately 240 samples per hour (one sample per 15 seconds) can be assayed using the automated protocol. We were encouraged by the data received and we expect further interest in the practical performance of such automation. As a mean of testing the robustness of our method, in the next step of our study, oxidative status was assessed in model cell lines derived from prostate cancer (PC-3, PNT1A and 22RV1) that were cultured on ellipticine (0, 0.5, 1, 1.5, 2, 2.5, 5, 7.5, 10, 15 μmol/l) supplemented agar. Antioxidant activity was assessed (DPPH, ABTS, FRAP, DMPD, FR) and calculated on the phenolic antioxidant level (rutin, quercitrin, ferulic and gallic acid), and thus an estimation was formulated of the oxidative stress as a result of the impact of anti-cancer drugs. It can be demonstrated that the new method has wide applicability.

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Antioxidant efficacy of flavonoid-rich fraction from Spermacoce hispida in hyperlipidemic rats

Cited by 23 publications

References 27 publications

Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats

Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats

Antihyperlipidemic Efficacy of Flavonoid-Rich Fraction From Spermacoce Hispida Seed on High-Fat-Diet Fed Rats: A Dose-Dependent Study

Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

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