Antioxidant effects of supplementation of 3,4‐dihydroxyphenyl glycol on sperm parameters and oxidative markers following cryopreservation in canine semen

Abstract

Supplementing the extender with antioxidants with low molecular weight can enhance the quality of the post-thaw sperm during the freezing process. This study was aimed at determining the impacts of 3,4-dihydroxyphenyl glycol (DHPG) on the spermatozoa of the canine undergoing freeze-thawing process. In this study, 24 ejaculates were obtained from three mixed-breed dogs and were diluted in a Trisbased extender. The diluted semen was divided into aliquots for supplementation of 10, 30, 50 and 70 µg/ml of DHPG, control (without antioxidant) and control sham (DMSO). After being extended, the semen was equilibrated at a temperature of 4°C and then transferred to the straws and kept 4 cm above the liquid nitrogen for 20 min and was finally immersed in the liquid nitrogen. They were cryopreserved for seven days; then, sperm parameters including sperm motility evaluation, motility characteristics, viability, DNA and plasma membrane integrity, total antioxidant capacity (TAC), reduced glutathione content (GSH), antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT] and glutathione peroxidase [GPx]) activity malondialdehyde (MDA) levels were evaluated. This study showed that spermatozoa cryopreservation with 50, 30 and 70 µg/ml of DHPG concentrations had better progressive motility, Curvilinear Velocity, Linearity, viability, intact plasma membrane and the levels of TAC, GPx and GSH were higher than the control group. The 50, 30 and 70 µg/ml of DHPG concentrations led to the significant decrease of DNA damage compared to the control group. Total motility, average path velocity, straight-line velocity and CAT activity were significantly improved in 30 and 50 µg/ml of DHPG concentrations, compared to the control group. Also, the 50 and 30 µg/ml of DHPG concentrations, decreased MDA levels compared to the other groups, significantly. In conclusion, our study showed that the addition 50 µg/ml of DHPG to the canine semen extender improved the semen characteristics and oxidative markers in the cryopreservation process.

Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull

“…However, the values of linear distance ( μ m/s) and distance average path ( μ m/s) were higher in 5 mM glycine concentrations as compared to other glycine concentrations and control. The current study findings are in agreement with [ 41 , 42 ] in which they concluded that adding 25 mM glycine, glutamine, and 5 mM cysteine extender enhanced post-thawed motility, and improved plasma membrane and acrosome integrity of buffalo bull semen. In another study, Domosławska et al [ 43 ] reported antioxidant capability of vitamin E, vitamin C, and its combined effect (vitamin E + C) on semen quality parameters, i.e., motility, percentage of live spermatozoa, percentage of abnormal spermatozoa, and acrosome intactness in the cryopreserved Bhadawari bull semen.…”

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

“…More suitable energy sources for frozen-thawed sperm have also been examined [ 16 , 76 ]. In addition, the effects of spermine [ 77 ], kinetin [ 78 ], cholesterol-loaded cyclodextrin [ 79 ], iodixanol [ 80 ], metformin [ 81 ], stem cells [ 82 ], stem cell-conditioned medium [ 83 ], norepinephrine metabolite [ 84 ], and insulin-like growth factor-1 [ 85 ] have been examined. The effects of each treatment on the in vitro motility of frozen-thawed spermatozoa have been documented.…”