Antioxidant effects of flavonoids ofAnthriscus sylvestris in lard

Abstract

Ethanol/water (7:3) extracts of the plant species Anthriscus sylvestris possess antioxidant activity. Separation and identification of antioxidant components by thin-layer and column chromatography and spectral analysis demonstrated that quercetin and apigenin appeared to be the main flavonoid species in Anthriscus sylvestris. Rutin was one of the major quercetin glycosides. Structures of the isolated compounds were determined by infrared and 1H nuclear magnetic resonance spectroscopies. Ethanolic extract (70%) of A. sylvestris showed concentration-dependent, strong antioxidant activity as determined by the Schaal Oven test of lard at 60~ Rancimat analysis at 100~ showed that the antioxidant activity of 70% ethanolic extract of A. sylvestris was superior to quercetin, apigenin, or a tocopherol mixture.JAOCS 73, 773-776 (1996).KEY WORDS: Anthriscus sylvestris, antioxidant activity, apigenin, flavonoids, quercetin, rutin, tocopherol.The possible toxicity of synthetic antioxidants (1,2) has increased interest in preparing antioxidants from natural products by extraction, fractionation, and purification. Flavonoids are of particular interest among naturally occurring substances with potential antioxidant activity. In addition to essential oils, flavonoids are characteristic constituents of a great number of species of the Apiaceae (3). The Serbian plant species Anthriscus sylvestris, family Apiaceae, has been widely used in folk medicine as tonics, diuretics, and salad dressing (4,5). A preliminary study of its chemical composition was made on lignans of the root (6) and essential oils, fatty acids, and sterols of the flowers and leaves (7). Chemical analysis of the aerial parts ofA. sylvestris, collected in the vicinity of Belgrade, have shown the presence of three flavonoid compounds--apigenin, quercetin, and rutin (8). It is known that flavonoids are not only soluble in water-alcoholic mixtures, but also have high solubility in fats and oils, which can promote their use as antioxidants in edible oils (9).As natural sources of antioxidants are gaining importance for food technology (! 0), the present study was initiated to identify flavonoids in A. sylvestris with antioxidant activity and to evaluate their potential as antioxidants.

Green tea extract as food antioxidant. Synergism and antagonism with α-tocopherol in vegetable oils and their colloidal systems

“…At the highest concentration of 1 lmol antioxidant/g oil, the antioxidant activity of the assayed samples, in a descending order, was epicatechin > catechin > GTE > TOH. This ranking is consistent with the result by Milovanovic, Picuric-Jovanovic, Vucelic-Radovic, and Vrbaski (1996), who reported that hydrophilic compounds were more efficient in lard systems than hydrophobic antioxidants, although, some studies have found that hydrophobic antioxidants are more effective in bulk oils than hydrophilic antioxidants (Porter, 1993).…”

Natural antioxidants from herbs and spices

“…Different flavonoids were also isolated from the extract of the sage Salvia nemorosa L. [130], an aromatic perennial herb of the southern European region, and from Anthriscus sylvestris [131], a Serbian plant species used in folk medicine and in salad dressing. The flavonoids, and especially flavonoid glycosides, can be found in nearly all herbs and spices tested [132].…”

Antitumor Constituents from Anthriscus Sylvestris (L.) Hoffm

“…This plant has a lignan named deoxypodophyllotoxin (Noguchi et al, 1940), which is known to have many bioactivities such as antitumor activity (Kozawa et al, 1978b;Ayres et al, 1990;Lim et al, 1999), anti-platelet aggregation activity, antiviral activity, antiproliferative activity, broad insecticidal activity, inhibition of passive cutaneous anaphylaxis reactions , liver protective action (Kiso et al, 1982), and anti-inflammatory activity (Lee et al, 2004). A. sylvestris contains highly lignans, phenylpropanoids, flavonoids, coumarins, organic acids, and so on (Tozaburo et al, 1979;Liang et al, 1990;Milovanovic et al, 1996;Ikeda et al, 1998;Bos et al, 2002;Koulman et al, 2007). Umbelliferae has high research value of anti-tumor activity.…”