Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar semen plasma

Abstract

The protective effect of vitamin E and reduced glutathione (GSH) against lipid peroxidation in boar semen plasma was studied. The lipid peroxidation, measured by the test for thiobarbituric acid reactive substances (TBARS), doubled in the presence of the lipid peroxidation Fe(2+)-sodium ascorbate-inducing system. The ascorbate-induced TBARS were inhibited by about 62% through the water-soluble vitamin E analog (TROLOX) and about 57% by GSH. In the in vivo experiments, 7 wk of oral DL-alpha-tocopherol acetate (1000 IU/d/animal) administration caused a significant fall in the level of the semen plasma TBARS, from 2.2 +/- 0.09 to 1.2 +/- 0.13 nmol MDA/mL. The semen plasma superoxide dismutase (SOD) and GSSG tended to increase with the time of vitamin E administration, but the increment did not reach a significant level by the seventh week. The vitamin E supplementation significantly increased the number of spermatozoa per 1 cm3 of ejaculate. The protective role of vitamin E and GSH with respect to boar semen against fatty acid peroxidation and a positive influence of vitamin E supplementation on semen quality have been evidenced.

Effect of storage for 24 h at 18°C on sperm quality and a comparison of two assays for sperm membrane lipid peroxidation

“…Similarly, the amount of LPO was increased after 24 h of storage with the increase being much larger for the low motility group. One factor influencing sperm motility and vitality is the production of ROS, which are known to induce lipid peroxidation of sperm membranes, inhibit sperm metabolism, deplete ATP, and cause leakage of intracellular enzymes (de Lamirande and Gagnon 1992;Brezezinska-Slebodzinska et al 1995;Cerolini et al 2000). The increased LPO noted in the present study agrees with previous reports where increased LPO was documented after storage of liquid boar semen (Kumaresan et al 2009).…”

“…This reduction in fertility and binding ability of fresh boar sperm likely involve changes in the sperm plasma membrane. Boar sperm are sensitive to peroxidation damage due to their high content of unsaturated fatty acids in the phospholipids of their plasma membranes (Cerolini et al 2000) and relatively low antioxidant capacity of boar seminal plasma (Brezezinska-Slebodzinska et al 1995). Sperm are efficient producers of reactive oxygen species (ROS; Cerolini et al 2000) and ROS may be one factor involved in the decreased motility and viability associated with the storage of sperm.…”

Oxidative Status of Boar Semen during Storage

“…Brezezińska-Slebodzińska et al (1995) presented that over short periods of incubation, vitamin E and GSH protects against lipid peroxidation reducing losses in the semen quality. Additional studies have shown that Trolox protects cryopreserved sperm decreasing lipid peroxidation (Minaei et al, 2012) and bovines (Hu et al, 2011), reducing motility losses.…”

Evidences for the Role of Melatonin as a Protective Additive During Buffalo Semen Freezing

“…Fluidity is linked to the integrity of the membrane lipids (Stubbs and Smith, 1984) and any change in lipid composition of the plasma membrane might therefore be associated with the cooling and storage effects. Lipid peroxidation might be one of the mechanisms responsible for the negative biochemical and physiological changes during sperm storage (Cerolini et al, 2000) and the relative low antioxidant capacity of seminal plasma (Brezezinska-Slevbodzinska et al, 1995).…”