Antioxidant effect of oleic acid and hydroxytyrosol in an experimental model similar to Huntington's disease

Abstract

Efecto antioxidante del ácido oleico e hidroxitirosol en un modelo experimental similar a la enfermedad de Huntington ResumenLos radicales libres juegan un papel fundamental en la alteración neuronal que tiene lugar en enfermedades neurodegenerativas y en el envejecimiento cerebral. Se ha asociado la dieta Mediterránea con la reducción del riesgo de padecer enfermedades neurodegenerativas. Objetivos. Este estudio fue diseñado para probar si el ácido oleico y el hidroxitirosol, componentes del aceite de oliva virgen-extra, ejercen un efecto antioxidante protector en el cerebro ante el estrés oxidativo inducido por el ácido 3-nitropropiónico. Métodos. El ácido 3-nitropropiónico se administró intraperitonealmente a una dosis de 20 mg/kg de peso durante cuatro días consecutivos. El ácido oleico (con una dosis del 4% de calorías ingeridas diariamente por el animal) y el hidroxitirosol (2,5 mg/kg de peso en solución acuosa) se administraron durante 14 días a ratas Wistar. Resultados. El ácido 3-nitropropiónico causó un aumento de la peroxidación lipídica y de la actividad de la glutatión peroxidasa, y una reducción en la concentración de glutatión reducido. Los resultados obtenidos además demuestran que el ácido oleico y el hidroxitirosol reducen los niveles de los productos de peroxidación lipídica y bloquean el agotamiento del glutatión reducido en el cerebro tras la administración de ácido 3-nitropropiónico. Conclusión. Tanto el ácido oleico como el hidroxitirosol actúan como potentes antioxidantes frente al estrés oxidativo inducido por el ácido 3-nitropropiónico. AbstractFree radicals play an important role in neuronal alterations during the progression of neurodegenerative disorders and brain aging. Mediterranean-style diet has been associated to a reduction in the risk to develop neurodegenerative disorders. Objectives. This study was designed to test whether oleic acid and hydroxytyrosol, both components of extra-virgin olive oil, exert an antioxidant and protective effect in the brain subjected to oxidative stress induced by 3-nitropropionic acid. Methods. 3-Nitropropionic acid was administered intraperitoneally to animals at a dose of 20 mg/kg for 4 consecutive days. Oleic acid (given at a dose equivalent to 4% of the calories ingested every day per animal) and hydroxytyrosol (2,5 mg/kg, dissolved in aqueoussolution) were administered during 14 consecutive days to Wistar rats. Results. 3-Nitropropionic acid caused increased levels of lipid peroxidation and glutathione peroxidase activity, and a depletion in the concentration of reduced glutathione. Our findings also demonstrate that oleic acid and hydroxytyrosol reduce the levels of lipid peroxidation and prevent the depletion of reduced glutathione in the brain induced by the administration of 3-nitropropionic acid. Conclusion. Both oleic acid and hydroxytyrosol act as potent antioxidants against the oxidative damage induced by 3-nitropropionic acid.

Estudo da eficiência do Orbignya oleifera como inibidor verde de corrosão para aço com baixo teor de carbono comparado com inibidor comercial em solução HCl 1M

“…A eficiência encontrada nas concentrações de 3 e 4 g/L pode estar associada a composição química desse óleo, na qual consta a presença do ácido láurico com concentração de 47% no óleo de babaçu, o que pode ter contribuído para uma camada de inibição mais eficiente. Estudo semelhante foi encontrado por Machado [26], além da ação do ácido oléico, presente neste óleo na concentração de 12 a 16%, como mostra as pesquisas realizadas por Alconchel-Gago [33].…”

Maternal rat prenatal and neonatal treatment with pequi pulp reduces anxiety and lipid peroxidation in brain tissue of rat offspring at adolescence

“…The brain is particularly vulnerable to oxidative damage, due to its high oxygen consumption, high content of polyunsaturated fatty acids, and mitochondrial activity. Compared to other organs and tissues, the brain presents low antioxidant capacity [ 17 ]. In brain cell membranes, stress alters both composition and function, and it reduces dendrite growth in the hippocampus, amygdala, and prefrontal cortex, all of which are involved in anxiety and depressive behaviors [ 18 ].…”

Multiscale design of a dairy beverage model composed of Candida utilis single cell protein supplemented with oleic acid

“…In addition, supplementation with 0.28 mg of OA in Swiss-Webster mice diet has been shown to prevent organ dysfunction and mortality in experimental sepsis (Gonçalves- de-Albuquerque et al, 2016). Interestingly, a 4% OA supplementation in the total calories consumed by Wistar mice showed an antioxidant effect, generating a neurodegenerative oxidation effect in a Huntington's disease experimental model (Alconchel-Gago et al, 2014). However, given that OA is susceptible to oxidation by oxygen or light, it might Multiscale design of a dairy beverage model composed of Candida utilis single cell protein supplemented with oleic acid be necessary to use innovative technics to improve its thermostability, as well as maintain its functional and biological activity (López Hernández, 2010;Bakry et al, 2016).…”