Antioxidant effect of nondigestible levan and its impact on cardiovascular disease and atherosclerosis

Dahech, Imen; Harrabi, Bahira; Hamden, Khaled; Feki, Abdelfattah El; Mejdoub, Hafedh; Belghith, Hafedh; Belghith, Karima

doi:10.1016/j.ijbiomac.2013.04.058

Cited by 53 publications

(14 citation statements)

References 33 publications

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“…It is an ideal soluble dietary fiber (Peshev and Van den Ende 2014) which is nondigestible and presents favorable prebiotic properties ). In addition, it exhibits antiobesity (Oh et al 2014), antioxidant (Dahech et al 2013), hypocholesterolemic (Yamamoto et al 1999), hypolipidemic , fibrinolytic (Esawy et al 2013), immunostimulatory (Xu et al 2006), and antitumor effects . Furthermore, levan is also a potential substitute for gum arabic due to its low viscosity and high water solubility (Han 1990).…”

Section: Levan Biosynthesismentioning

confidence: 99%

Recent novel applications of levansucrases

Zhang

et al. 2015

Appl Microbiol Biotechnol

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Levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule, including polymerization, transfructosylation, and hydrolysis. As a key biocatalyst in the synthesis of levan and levan-type fructooligosaccharides, levansucrase has been widely and intensively studied. Due to the promising physiological effects of levan and levan-type fructooligosaccharides, they exhibit great potential in food and pharmaceutical industries. Another important point that attracts much attention is the wide substrate specificity of levansucrase toward monosaccharides, disaccharides, and aromatic and alkyl alcohols, producing diverse sucrose analogues, hetero-oligosaccharides (especially lactosucrose), and interesting fructosides. The present article summarizes and discusses the recent applications of microbial levansucrase in detail.

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Section: Levan Biosynthesismentioning

confidence: 99%

Recent novel applications of levansucrases

Zhang

et al. 2015

Appl Microbiol Biotechnol

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“…It harms the endothelium, attracts monocytes and promotes foam cell formation. It is therefore predicted that antioxidants will be therapeutically beneficial in treating human atherosclerotic vascular disease[15]. The aim of this study was to determine the antioxidant potencies of HDL,which improve the antioxidant activities and to recognize the fundamental mechanisms of the antioxidant properties.In the FTC test, the capacity of HDL to hinder lipid peroxidation is evaluated.…”

mentioning

confidence: 99%

Antioxidant activity of high-density lipoprotein (HDL) using different in vitro assays

Yusoff

Bakar

Muid

et al. 2018

J. Fundam and Appl Sci.

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The role of HDL in reverse cholesterol transport is well known but other atheroprotective mechanism, e.g. antioxidant capacities of HDL is not clear. The aim of this study is to determine the effects of different doses of HDL on antioxidant activities assay. HDL was isolated from plasma by single step doses of HDL were measured by ferric thiocyanate (FTC) (DPPH) and dichlorofluorescein (DCF) lag time and lower reaction rate in a dose HDL had inhibitory effects on radical oxygen free radical scavenging activities. HDL is a potent antioxidant in terms of inhibition of lipid peroxidation, ROS production and LDL oxidation. These may to some extent add to the antiatherogenic beyond reverse

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“…Tamer I. M. Ragab et al suggested that levan could play a fundamental role in solving peptic ulcer problems [7]. Several other studies have confirmed that levan has prebiotic characteristics [7][8][9] and antioxidative [10], antiobesity [11], antifungal [12], antidiabetes [13], and antitumor [14] effects. Because of the excellent properties and application prospects of levan, it is urgent for researchers to improve its yield and quality.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of Levansucrase Gene of Bacillus velezensis BM-2 and Enzymatic Synthesis of Levan

Zhang

Zhao

et al. 2021

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Levan is a versatile and valuable fructose homopolymer, and a few bacterial strains have been found to produce levan. Although levan products have numerous specific functions, their application and promotion were limited by the production capacity and production cost. Bacillus velezensis BM-2 is a levan-synthesizing strain, but its levan production is too low to apply. In this study, the levansucrase gene of B. velezensis BM-2 was cloned to plasmid pET-32a-Acma-zz, and the recombinant plasmids were transferred to Escherichia coli BL21. A transformed clone was selected to express and secrete the fusion enzymes with an Acma-tag efficiently. The expressed products were further purified by a self-developed separating material called bacterial enhancer matrix (BEM) particles. The purification efficiency was 93.4%, with a specific activity of 16.589 U/mL protein. The enzymatic reaction results indicated that the optimal reaction temperature is 50 °C, the optimal pH of the acetate buffer is 5.6, and the buffer system greatly influenced the enzyme activity. The enzyme activity was enhanced to 130% in the presence of 5 mM Ca2+, K+, Zn2+, and Mn2+, whereas it was almost abolished in the case of Cu2+ and Fe3+. The values of Km, kcat, and kcat/Km were 17.41 mM, 376.83 s−1, and 21.64 mM−1s−1, respectively. The enzyme amount of 20 U/g sucrose was added to the system containing 400 g/L sucrose, and the levan products with a concentration of 120 g/L reached after an incubation of 18 h, which was 8 times that of the yield before optimization. The results of molecular docking analysis indicated that the Asp86 might act as a nucleophilic catalytic residue for sucrose, Arg246 and Asp247 act as transition state stabilizer of transfructosylation, and Glu340 and Arg306 were recognized as general acid donors. They formed the catalytic-groups triad. The unique properties and catalytic activity of the levansucrase suggest that it deserves further research and might have good industrial application prospects.

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Antioxidant effect of nondigestible levan and its impact on cardiovascular disease and atherosclerosis

Cited by 53 publications

References 33 publications

Recent novel applications of levansucrases

Recent novel applications of levansucrases

Antioxidant activity of high-density lipoprotein (HDL) using different in vitro assays

Cloning and Expression of Levansucrase Gene of Bacillus velezensis BM-2 and Enzymatic Synthesis of Levan

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