Antioxidant effect of mesalazine in the experimental colitis model induced by acetic acid

Abstract

Introduction Inflammatory bowel disease (IBD) is characterized by a chronic inflammation of the gastrointestinal tract, without specific cause or pathogen. Objective The effect of mesalazine in a colitis model induced by acetic acid (AA) was evaluated. Methods We used 40 Wistar rats, ±350 g, divided into 4 groups: control (CO); control + mesalazine (CO + M); colitis (CL) and colitis + M (CL + M) at 24 and 48 h of treatment. The animals received the substances by an intracolonic enema of AA 4% and treatment with mesalazine PO 20 mg/kg after colitis induction. Results Mesalazine reduced tissue damage in the gut, normalized sphincter anal pressure levels and decreased lipid peroxidation, metabolites of nitric oxide and iNOS and NF-kB expression in the treated groups in both treatment time points (24 and 48 h), as well as the activity of antioxidant enzymes. Conclusion Mesalazine was effective in reducing tissue damage and oxidative and inflammatory damage, restored antioxidant capacity and increased anal sphincter pressure levels, possibly due to its antioxidant effect.

Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms

“…In line with the activation of an endogenous defence mechanism, idebenone increased the NQO-1 expression and SOD activity rate in our study ( Figure 6). Previous reports associated elevated levels of NO with the severity of colitis in UC patients and in animal models [58][59][60]. In line with idebenone-dependent protection, idebenone also influenced RNS by reducing NO levels in our study.…”

“…Therefore, at present, it is not possible to align the multiple contradicting results of the reported clinical trials. On the other side, a plethora of literature supports the role of oxidative stress in UC and consequently justifies the use of antioxidants as a therapeutic strategy [15,27,52,54,60,[98][99][100][101][102]. Since UC is a multifactorial disease, pharmacological targeting of individual factors appears of limited use to provide effective and sustainable therapy outcomes.…”

Animal models for inducing inflammatory bowel diseases: integrative review

“…Nesta pesquisa, os modelos químicos de indução encontrados nos dez artigos selecionados foram DSS, TNBS e ácido acético, os quais estão entre os modelos químicos de indução mais utilizados na prática, seja por seu baixo custo, fácil execução e/ou boa reprodutibilidade [21][22][23][24][25][26][27][28][29] .…”

“…Dois dos artigos analisados utilizaram o DSS como modelo de indução de colite, mas apenas um especificou seu estudo para a UC. No estudo realizado por Oliveira et al (2014), a UC foi especificada, observando-se uma série de manifestações clínicas características da doença, a saber diarreia, presença de sangramento retal, diminuição de peso dos animais, destruição direta das células epiteliais, com mudança na expressão de metaloproteinases de matriz extracelular (MMP), além de aumento dos níveis de MPO 27,29 .…”

“…Os modelos químicos de colite são em sua maioria utilizados para testar mecanismos imunológicos de doenças ou para testar novas alternativas terapêuticas 21,27 . A relativa facilidade e velocidade de estabelecer a inflamação pelos modelos quimicamente induzidos os torna mais úteis para o estudo dos efeitos de pequenas moléculas terapêuticas de vida curta que precisam provar seu caráter imunogênico.…”