Antioxidant effect of Lactobacillus fermentum HFY02-fermented soy milk on D-galactose-induced aging mouse model

Effects of Lactobacillus plantarum HFY15 on Lupus Nephritis in Mice by Regulation of the TGF-β1 Signaling Pathway

“…Finally, the tissues were stained with hematoxylin and eosin (H&E, Shanghai Shangbao Biotechnology Co., Ltd, Shanghai, China), and histopathological changes were observed by light microscope (BX43, Olympus Corporation, Tokyo, Japan). 17 …”

“…Finally, the tissues were stained with hematoxylin and eosin (H&E, Shanghai Shangbao Biotechnology Co., Ltd, Shanghai, China), and histopathological changes were observed by light microscope (BX43, Olympus Corporation, Tokyo, Japan). 17 qPCR Experiment First, 0.9 mL of normal saline was added to 0.1 g of mouse kidney tissue, and the tissue mixture was homogenized. Then, RNAzol (1.0 mL, Invitrogen, New York, USA) was used to extract RNA from mouse kidney tissue.…”

The recovery effect of Feixingcao tea (Teucrium viscidum Bl.) on inhibiting the decreased exercise ability caused by chronic alcoholic liver injury

“…Then, using a StepOnePlus real-time PCR machine (Thermo Fisher Scientific, Waltham), a mixture of 10 μL SYBRGreen PCR Master Mix, 1 μL upstream primer and 1 μL downstream primer, 1 μL cDNA template, and 7 μL DEPC was amplified. The relative expression level of each gene was then determined using the 2 -ΔΔCT technique, where CT stood for cycle threshold and β-actin was utilized as an internal reference gene (Hu et al, 2022;Long et al, 2022).…”

Effect of gardenia tea on apoptosis of human thyroid cancer cell line SW579 through PI3K-Akt pathway

“…The cells were synthesized at 4 °C for 3 min, and then the expression level was detected by qRT-PCR (StepOne Plus, Thermo Fisher Scientific). Then, 2 μL of cDNA was synthesized, 1 μL of upstream and downstream primers at a concentration of 10 μmol/L, 10 μL of SYBR Premix Ex Taq II (2×), 0.4 μL of ROX reference Dye (50 ×), and 5.6 μL of Sterilized double-distilled water was mixed, and then placed in a quantitative PCR machine for the reaction (Hu et al, 2022;Long et al, 2022).…”