Antioxidant effect of inorganic ions on UVC and UVB induced lipid peroxidation

Costanzo, L.L.; Guidi, Guido De; Giuffrida, Salvatore; Sortino, Salvatore; Condorelli, G.

doi:10.1016/0162-0134(94)00048-f

Cited by 31 publications

(12 citation statements)

References 18 publications

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“…In the UVB experiment, cell survival was higher with liposome DsMnSOD than PTD-DsMnSOD and the cell survival of empty liposomes was marginally higher than those treated with PBS. We hypothesize that this was due to the protective effect of liposomes on cells when UVB was applied, as indicated by previous studies (36)(37)(38).…”

Section: Discussionsupporting

confidence: 52%

Effect of superoxide dismutase-entrapped liposomes and protein transduction domain-superoxide dismutase on human umbilical vein endothelial cells

Zhang

Cao

Xie

et al. 2014

Molecular Medicine Reports

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Superoxide dismutases (SOD) are able to remove the superoxide anion free radicals produced by environmental stress and thereby protect cells from being injured by reactive oxygen species. However, SOD is unable to transduce automatically across cell membranes. Protein transduction domains (PTDs) are peptides able to mediate protein delivery into cells and were first observed in the HIV‑1 Tat protein. In the present study, PTD (RKKRRQRRR) was fused to Dunaliella salina (Ds)MnSOD to form PTD‑DsMnSOD. This was inserted into pET32a to construct the recombinant plasmid pET32a‑PTD‑DsMnSOD and transduced into E. coli BL21(DE3) to obtain purified PTD‑DsMnSOD proteins. Liposome‑encapsulated proteins are also able to cross cell membranes. In this study, DsMnSOD proteins were purified and encapsulated by liposomes. The obtained MnSOD, PTD‑MnSOD and liposome MnSOD were used to protect human umbilical vein endothelial cells (HUVECs) from injury under oxygen pressure. A cell counting kit 8 was used to test the survival rate of HUVECs and results indicated that the protective effect of MnSOD was limited compared with that of PTD‑MnSOD and liposome MnSOD. Thus, PTD and liposomes exhibited improved effects when MnSOD was present in cells.

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Section: Discussionsupporting

confidence: 52%

Effect of superoxide dismutase-entrapped liposomes and protein transduction domain-superoxide dismutase on human umbilical vein endothelial cells

Zhang

Cao

Xie

et al. 2014

Molecular Medicine Reports

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“…(i) UV radiation can be absorbed directly by cellular components, inducing photoinduced reactions that can cause cellular damage. (ii) In addition, when UV radiation is absorbed by cellular materials acting as photosensitizers, photoionization can cause the formation of excited states or radicals that can cause cellular damage (47)(48)(49). Meanwhile, reactive oxygen species (ROS) are one of the major causes of lipid peroxidation that produce physical changes in the cell membrane (50-52).…”

Section: Resultsmentioning

confidence: 99%

Application of a Krypton-Chlorine Excilamp To Control Alicyclobacillus acidoterrestris Spores in Apple Juice and Identification of Its Sporicidal Mechanism

Kang

Hong

Kang

2020

Appl Environ Microbiol

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The aim of this study was to investigate the sporicidal effect of a krypton-chlorine (KrCl) excilamp against Alicyclobacillus acidoterrestris spores and to compare its inactivation mechanism to that of a conventional UV lamp containing mercury (Hg). The inactivation effect of the KrCl excilamp was not significantly different from that of the Hg UV lamp for A. acidoterrestris spores in apple juice despite the 222-nm wavelength of the KrCl excilamp having a higher absorption coefficient in apple juice than the 254-nm wavelength of the Hg UV lamp; this is because KrCl excilamps have a fundamentally greater inactivation effect than Hg UV lamps, which is confirmed under ideal conditions (phosphate-buffered saline). The inactivation mechanism analysis revealed that the DNA damage induced by the KrCl excilamp was not significantly different (P > 0.05) from that induced by the Hg UV lamp, while the KrCl excilamp caused significantly higher (P < 0.05) lipid peroxidation incidence and permeability change in the inner membrane of A. acidoterrestris spores than did the Hg UV lamp. Meanwhile, the KrCl excilamp did not generate significant (P > 0.05) intracellular reactive oxygen species, indicating that the KrCl excilamp causes damage only through the direct absorption of UV light. In addition, after KrCl excilamp treatment with a dose of 2,011 mJ/cm2 to reduce A. acidoterrestris spores in apple juice by 5 logs, there were no significant (P > 0.05) changes in quality parameters such as color (L*, a*, and b*), total phenolic compounds, and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity. IMPORTANCE Alicyclobacillus acidoterrestris spores, which have high resistance to thermal treatment and can germinate even at low pH, are very troublesome in the juice industry. UV technology, a nonthermal treatment, can be an excellent means to control heat-resistant A. acidoterrestris spores in place of thermal treatment. However, the traditionally applied UV sources are lamps that contain mercury (Hg), which is harmful to humans and the environment; thus, there is a need to apply novel UV technology without the use of Hg. In response to this issue, excilamps, an Hg-free UV source, have been actively studied. However, no studies have been conducted applying this technique to control A. acidoterrestris spores. Therefore, the results of this study, which applied a KrCl excilamp for the control of A. acidoterrestris spores and elucidated the inactivation principle, are expected to be utilized as important basic data for application to actual industry or conducting further studies.

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“…Agents that induce oxidative damage can produce lipid peroxidation in the cell membrane (43). Although UV primarily produces its toxicity through DNA damage, it also produces some lipid damage in the membrane (44). Lowering of lipid peroxidation of membrane after UVC or H 2 O 2 treatment follows as a natural consequence of lower level of ROS produced in these cells.…”

Section: Discussionmentioning

confidence: 99%

UV Released Factors Induce Antioxidant Defense in A375 Cells

Ghosh¹,

Guha

Bhowmik

2012

Photochem & Photobiology

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UV light leads to release of different secretory factors from irradiated cells of which some of them have been characterized. We have reported earlier that cells exposed to the supernatant medium from irradiated cells were resistant to killing by some genotoxic agents. In this study, we present our finding that demonstrates DNA damage induced by UV or H(2)O(2) is lowered on prior exposure to the UV released factors (UVRF). Production of ROS in cells and lipid peroxidation was also lowered. It was found that treatment of unexposed cells with UVRF present in the supernatant medium altered the antioxidant defense activity in cells. Significant was the increase in catalase (CAT) and Cu-Zn superoxide dismutase (SOD) activity, whereas glutathione peroxidase (GPx) and reduced glutathione (GSH) levels remained unaffected. Cells exposed to UVRF prior to UV or H(2)O(2) treatment also experienced such upregulation; however, the remarkable increase in the GPx activity exhibited by these cells was not observed in cells exposed to H(2)O(2) or UV alone. It appears that exposure to UVRF tinkered with antioxidant defense in cells to facilitate its proliferation upon assault by an agent that can produce oxidative damage.

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Antioxidant effect of inorganic ions on UVC and UVB induced lipid peroxidation

Cited by 31 publications

References 18 publications

Effect of superoxide dismutase-entrapped liposomes and protein transduction domain-superoxide dismutase on human umbilical vein endothelial cells

Effect of superoxide dismutase-entrapped liposomes and protein transduction domain-superoxide dismutase on human umbilical vein endothelial cells

Application of a Krypton-Chlorine Excilamp To Control Alicyclobacillus acidoterrestris Spores in Apple Juice and Identification of Its Sporicidal Mechanism

UV Released Factors Induce Antioxidant Defense in A375 Cells

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