Antioxidant Effect ofLactobacillus acidophilusas a Probiotic at Different Time Intervals

Abstract

Background: Probiotics are survival microorganisms that, when administered in sufficient amounts, confer health benefits to the host and can be used in an antioxidative role. Objectives: The antioxidative effect of whole cells and intracellular cell-free extracts of the lactic acid bacteria Lactobacillus acidophilus (PTCC 1643) as a probiotic at three different time intervals was investigated. Materials and Methods: Antioxidant biomarkers, such as total antioxidant power (TAP), measured with the FRAP (ferric-reducing ability of plasma) method, were evaluated at 24, 48, and 72 hours. Results:The results showed that extracts and bacteria of L. acidophilus were able to significantly increase TAP after 24 and 72 hours. Conclusions:The results showed that the effect of L. acidophilus is time-dependent.

In vitro assessment of metabolic profile of Enterococcus strains of human origin

“…The cell-free supernatants of lactic acid bacteria have already proved their scavenging properties and thus have reduced the risk of chronic diseases [52]. The scavenging property of probiotics can be attributed to metal chelation, ROS radical scavenging, or ascorbate auto-oxidation activity [53]. The ROS theory proposes the development of antioxidative agents that would help in curbing the progression of free radical-related disorders.…”

Proteolysis and therapeutic potential of bioactive peptides derived from Cheddar cheese

“…The peptide sequence of Leu-Leu-Pro-His-His also exerts antioxidant activity in probiotic Cheddar cheese (Kitts & Weiler, 2003).The sequence of the peptide plays a crucial role in deriving highly potential antioxidant peptides, which can be effectively used in several food applications (López-Expósito et al, 2017). Nazari et al (2016) conducted trials on Lactobacillus acidophilus (probiotic adjunct) to access total antioxidant power (TAP) using the ferric reducing ability of plasma (FRAP). The study was conducted after 24, 48, and 72 h in bacterial samples and extracts.…”

“…Nazari et al ( 2016 ) conducted trials on Lactobacillus acidophilus (probiotic adjunct) to access total antioxidant power (TAP) using the ferric reducing ability of plasma (FRAP). The study was conducted after 24, 48, and 72 h in bacterial samples and extracts.…”